Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CKV74_RS00410 Genome accession   NZ_LT906463
Coordinates   83253..83702 (+) Length   149 a.a.
NCBI ID   WP_007241769.1    Uniprot ID   -
Organism   Haemophilus pittmaniae strain NCTC13334     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 78253..88702
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV74_RS00380 (NCTC13334_00077) tadA 78465..78935 (-) 471 WP_094188627.1 tRNA adenosine(34) deaminase TadA -
  CKV74_RS00385 (NCTC13334_00078) - 79050..79901 (-) 852 WP_007241538.1 thymidylate synthase -
  CKV74_RS00390 (NCTC13334_00079) lgt 79914..80723 (-) 810 WP_007241655.1 prolipoprotein diacylglyceryl transferase -
  CKV74_RS00395 (NCTC13334_00080) - 80732..81526 (-) 795 WP_007241724.1 sulfite exporter TauE/SafE family protein -
  CKV74_RS00400 (NCTC13334_00081) rppH 81526..82110 (-) 585 WP_007241732.1 RNA pyrophosphohydrolase -
  CKV74_RS10615 - 82273..82407 (-) 135 WP_269457018.1 hypothetical protein -
  CKV74_RS00405 (NCTC13334_00082) ampD 82577..83125 (-) 549 WP_007241547.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CKV74_RS00410 (NCTC13334_00083) pilA 83253..83702 (+) 450 WP_007241769.1 prepilin peptidase-dependent pilin Machinery gene
  CKV74_RS00415 (NCTC13334_00084) pilB 83702..85072 (+) 1371 WP_007241714.1 GspE/PulE family protein Machinery gene
  CKV74_RS00420 (NCTC13334_00085) pilC 85072..86292 (+) 1221 WP_095176600.1 type II secretion system F family protein Machinery gene
  CKV74_RS00425 (NCTC13334_00086) - 86289..86957 (+) 669 WP_007241801.1 A24 family peptidase -
  CKV74_RS00430 (NCTC13334_00087) coaE 87018..87653 (+) 636 WP_007241821.1 dephospho-CoA kinase -
  CKV74_RS00435 (NCTC13334_00088) yacG 87646..87852 (+) 207 WP_007241731.1 DNA gyrase inhibitor YacG -
  CKV74_RS00440 (NCTC13334_00089) - 87849..88109 (+) 261 WP_095176601.1 GNAT family N-acetyltransferase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15552.78 Da        Isoelectric Point: 8.8103

>NTDB_id=1147702 CKV74_RS00410 WP_007241769.1 83253..83702(+) (pilA) [Haemophilus pittmaniae strain NCTC13334]
MKPSLFTARHKGFTLIELMIVIAIIAILATIAIPSYQNYTKKAAVSELLQAAAPYKGDIELCVYSTGKTADCDGGKNGIS
NNLTSAKGYVKSISVKSGAITVNGNNSLDGVQFTMQASGDSSTGVTWTTTCDANNTQLFPAGFCSAKGK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1147702 CKV74_RS00410 WP_007241769.1 83253..83702(+) (pilA) [Haemophilus pittmaniae strain NCTC13334]
ATGAAACCGAGTCTCTTTACCGCTCGCCACAAAGGCTTTACTTTAATTGAATTAATGATCGTTATTGCGATTATTGCCAT
TCTCGCCACAATTGCGATTCCGTCCTACCAAAATTACACTAAAAAAGCAGCTGTTTCTGAATTATTACAGGCTGCCGCTC
CCTATAAAGGCGATATTGAGCTCTGCGTGTATAGCACCGGTAAAACTGCCGATTGCGATGGCGGCAAAAATGGTATTAGT
AACAATCTCACCTCCGCAAAAGGCTATGTAAAAAGTATCAGTGTAAAAAGCGGTGCTATTACGGTAAACGGTAACAATAG
CTTGGATGGGGTGCAATTTACTATGCAGGCAAGTGGCGACAGCAGTACCGGCGTAACTTGGACAACTACCTGTGATGCCA
ATAATACTCAATTATTCCCTGCCGGTTTCTGCTCCGCTAAGGGTAAATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-142)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae 86-028NP

66.667

96.644

0.644
  pilA Haemophilus influenzae Rd KW20

65.278

96.644

0.631
  pilA Glaesserella parasuis strain SC1401

52.98

100

0.537
  pilA Vibrio campbellii strain DS40M4

39.437

95.302

0.376
  pilA2 Legionella pneumophila strain ERS1305867

42.188

85.906

0.362
  pilA2 Legionella pneumophila str. Paris

42.188

85.906

0.362

Multiple sequence alignment