Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   CKW05_RS08040 Genome accession   NZ_LT906457
Coordinates   1770551..1771777 (+) Length   408 a.a.
NCBI ID   WP_058484325.1    Uniprot ID   A0A0W0YYV5
Organism   Legionella spiritensis strain NCTC11990     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1765551..1776777
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW05_RS08020 (SAMEA4412668_01603) - 1766663..1767226 (-) 564 WP_058484329.1 hypoxanthine-guanine phosphoribosyltransferase -
  CKW05_RS08025 (SAMEA4412668_01604) - 1767320..1767652 (-) 333 WP_058484328.1 DUF1820 family protein -
  CKW05_RS08030 (SAMEA4412668_01605) - 1767784..1768557 (-) 774 WP_058484327.1 class I SAM-dependent methyltransferase -
  CKW05_RS08035 (SAMEA4412668_01606) pilB 1768820..1770544 (+) 1725 WP_058484326.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKW05_RS08040 (SAMEA4412668_01607) pilC 1770551..1771777 (+) 1227 WP_058484325.1 type II secretion system F family protein Machinery gene
  CKW05_RS08045 (SAMEA4412668_01608) - 1771831..1772694 (+) 864 WP_058484324.1 A24 family peptidase -
  CKW05_RS08050 (SAMEA4412668_01609) - 1772709..1773299 (-) 591 WP_058484323.1 sel1 repeat family protein -
  CKW05_RS08055 (SAMEA4412668_01610) - 1773311..1773763 (-) 453 WP_058484322.1 hypothetical protein -
  CKW05_RS08060 (SAMEA4412668_01611) - 1774094..1774909 (+) 816 WP_058484321.1 pyruvate, water dikinase regulatory protein -
  CKW05_RS08065 (SAMEA4412668_01612) - 1775083..1776528 (-) 1446 WP_058484320.1 bifunctional 2-methylcitrate dehydratase/aconitate hydratase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44817.57 Da        Isoelectric Point: 9.3817

>NTDB_id=1147565 CKW05_RS08040 WP_058484325.1 1770551..1771777(+) (pilC) [Legionella spiritensis strain NCTC11990]
MDKNKNANAIISYQWEGVNKTGEKVNGIIDARSLALAKADLRKQGIITKKVIKKRKPLFDKKNKKITQGDITVFSRQMAT
MIEAGIPLVQSFDIVSKGQSNNRMRDLIDGVKKDVETGLTLAEALQKHPEYFNDLFCNLVDAGEKSGSLEIMLDKVATYK
EKIETIKKKIKKALTYPMAVLVVAFIVTAALLIFVVPQFQSLFEGFGADLPAMTKAVVELSHFFQAYWYLIFGVLGGLIY
SFVYAKNHSPNFAQTIDRSMLKFPVIGPILEKAAIARFSRTLSITFAAGLPLVDALKSVSGATGNILYAKATDKIREDVS
TGQQMQVAMDNTHLFPNMVIQMVAIGEESGALEQMLGKVADFYEEEVDNAVDSLSSLLEPIIMSILGILVGGLVVAMYLP
IFKLGSAV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1147565 CKW05_RS08040 WP_058484325.1 1770551..1771777(+) (pilC) [Legionella spiritensis strain NCTC11990]
ATGGATAAAAATAAAAATGCCAACGCCATAATCAGCTACCAATGGGAGGGCGTCAACAAAACGGGTGAAAAAGTCAATGG
CATCATTGATGCCAGAAGCCTGGCATTGGCAAAAGCGGACTTGCGTAAACAAGGCATCATTACTAAAAAAGTGATTAAAA
AAAGAAAACCCCTGTTTGACAAAAAAAACAAAAAAATAACCCAGGGCGACATCACTGTCTTTAGCCGACAGATGGCTACC
ATGATCGAGGCGGGTATTCCCCTGGTACAATCGTTCGATATTGTTTCCAAGGGACAGAGTAATAACCGTATGCGCGACTT
GATTGATGGCGTAAAAAAAGATGTGGAAACAGGGCTGACTTTAGCGGAAGCGTTGCAAAAACACCCCGAATACTTTAACG
ATCTGTTTTGTAATCTGGTCGATGCCGGTGAAAAATCAGGTTCTCTGGAAATTATGCTTGATAAGGTCGCCACCTATAAG
GAAAAAATTGAAACCATCAAGAAAAAAATTAAAAAAGCGCTCACATATCCCATGGCCGTACTGGTTGTGGCATTCATTGT
AACAGCCGCGTTATTAATCTTTGTAGTACCACAATTTCAATCGTTATTTGAAGGATTCGGAGCGGATCTTCCAGCCATGA
CCAAAGCGGTTGTGGAATTGTCTCACTTTTTCCAGGCGTATTGGTATCTTATATTCGGCGTGCTGGGCGGCCTCATTTAT
TCATTTGTCTATGCCAAAAATCATTCGCCAAATTTTGCGCAAACGATTGACCGCTCGATGCTTAAATTTCCGGTTATTGG
CCCCATTCTTGAAAAAGCGGCTATCGCACGCTTTTCCCGTACCTTATCCATTACCTTTGCAGCGGGTTTGCCTCTGGTTG
ACGCCCTGAAATCGGTATCCGGCGCTACAGGTAACATTCTCTACGCCAAGGCCACTGATAAAATCAGGGAGGATGTATCT
ACAGGCCAGCAAATGCAGGTTGCTATGGACAACACTCATTTGTTTCCCAACATGGTCATTCAAATGGTCGCTATCGGCGA
GGAGTCCGGAGCCCTGGAACAAATGCTTGGTAAAGTAGCTGATTTTTATGAGGAAGAAGTGGATAATGCGGTTGACTCTT
TAAGTAGTTTGCTGGAACCTATTATCATGTCAATTTTGGGTATCCTGGTCGGGGGATTGGTAGTTGCAATGTATCTGCCT
ATTTTCAAACTGGGTTCCGCCGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0YYV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

80.741

99.265

0.801
  pilC Pseudomonas stutzeri DSM 10701

57.323

97.059

0.556
  pilC Acinetobacter baylyi ADP1

55.062

99.265

0.547
  pilC Acinetobacter baumannii D1279779

54.815

99.265

0.544
  pilG Neisseria meningitidis 44/76-A

45.844

97.304

0.446
  pilG Neisseria gonorrhoeae MS11

45.592

97.304

0.444
  pilC Vibrio cholerae strain A1552

42.317

97.304

0.412
  pilC Vibrio campbellii strain DS40M4

41.562

97.304

0.404
  pilC Thermus thermophilus HB27

35.749

100

0.363

Multiple sequence alignment