Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CKW05_RS08035 Genome accession   NZ_LT906457
Coordinates   1768820..1770544 (+) Length   574 a.a.
NCBI ID   WP_058484326.1    Uniprot ID   A0A0W0YYT4
Organism   Legionella spiritensis strain NCTC11990     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1763820..1775544
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW05_RS08015 (SAMEA4412668_01602) - 1765444..1766577 (+) 1134 WP_058484330.1 HlyD family type I secretion periplasmic adaptor subunit -
  CKW05_RS08020 (SAMEA4412668_01603) - 1766663..1767226 (-) 564 WP_058484329.1 hypoxanthine-guanine phosphoribosyltransferase -
  CKW05_RS08025 (SAMEA4412668_01604) - 1767320..1767652 (-) 333 WP_058484328.1 DUF1820 family protein -
  CKW05_RS08030 (SAMEA4412668_01605) - 1767784..1768557 (-) 774 WP_058484327.1 class I SAM-dependent methyltransferase -
  CKW05_RS08035 (SAMEA4412668_01606) pilB 1768820..1770544 (+) 1725 WP_058484326.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKW05_RS08040 (SAMEA4412668_01607) pilC 1770551..1771777 (+) 1227 WP_058484325.1 type II secretion system F family protein Machinery gene
  CKW05_RS08045 (SAMEA4412668_01608) - 1771831..1772694 (+) 864 WP_058484324.1 A24 family peptidase -
  CKW05_RS08050 (SAMEA4412668_01609) - 1772709..1773299 (-) 591 WP_058484323.1 sel1 repeat family protein -
  CKW05_RS08055 (SAMEA4412668_01610) - 1773311..1773763 (-) 453 WP_058484322.1 hypothetical protein -
  CKW05_RS08060 (SAMEA4412668_01611) - 1774094..1774909 (+) 816 WP_058484321.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63226.36 Da        Isoelectric Point: 5.3278

>NTDB_id=1147564 CKW05_RS08035 WP_058484326.1 1768820..1770544(+) (pilB) [Legionella spiritensis strain NCTC11990]
MATAAEEQRLQGIAQLMVHENLLDKEMAMEYQNQAQANKQSLLQYLVANEVLSAEKIALSVANNFGVPLIDLDCIEPDTL
PTTLVNEKLIRRHAIIPLFTRGNHLYLATDDPSKQASLKEIQFHTGLHSNAIVVETNKLSKLIDKLLNEKESQGLTDYVD
DSSDLEGLEISAEDEEQDMDASSSANDDAPVVKFVNKILVEAIKNGVSDIHFEPYEKEYRIRYRQDGILTEVATPPVSLS
SRITSRIKIMSNLDISERRIPQDGRFKMKLSRTRAIDFRVSTCPTVGGEKVVMRILDPGSTKLGIEALGFNPDQRKHFLH
AIEKPQGMILVTGPTGSGKTVTLYTALNILNTKEANISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPSFNIASSVTLLIAQRLARRLCESCKAPRDDFTIES
LMELGYSNEEAKSLKPYKAVGCSQCTNGYRGRIGLFEVLPMSKAIGELIMKGGNSLDILKQAQSEGMLTIHESGLKKIQE
GMTTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1147564 CKW05_RS08035 WP_058484326.1 1768820..1770544(+) (pilB) [Legionella spiritensis strain NCTC11990]
ATGGCTACTGCTGCAGAAGAACAACGTTTACAAGGTATTGCCCAGCTCATGGTCCACGAGAATCTCCTGGATAAGGAGAT
GGCTATGGAATACCAGAATCAGGCTCAAGCCAATAAACAGAGTTTGCTGCAATATCTTGTGGCAAACGAAGTCTTGTCCG
CTGAAAAAATTGCGCTCAGTGTCGCAAATAATTTTGGTGTACCGCTCATCGACCTGGATTGCATAGAACCGGATACTTTG
CCAACCACCCTCGTGAATGAAAAATTAATACGGCGTCATGCCATTATTCCCCTATTCACCCGGGGCAATCATCTTTATCT
TGCTACCGACGATCCCAGCAAACAAGCCTCGTTAAAGGAAATCCAGTTTCATACGGGTCTTCATTCCAATGCGATCGTTG
TAGAGACGAACAAACTAAGCAAACTGATAGACAAACTGCTGAATGAAAAGGAAAGTCAGGGTTTAACCGATTATGTGGAT
GATTCCAGTGATCTTGAAGGGCTTGAAATCAGTGCGGAAGATGAAGAACAGGACATGGATGCCTCATCTTCGGCCAACGA
CGATGCACCCGTCGTAAAATTCGTCAACAAGATTCTGGTGGAAGCCATTAAAAACGGCGTTTCAGATATTCATTTTGAGC
CTTATGAGAAAGAATATCGTATACGATACCGTCAAGACGGCATTCTTACGGAAGTCGCCACCCCTCCCGTAAGTCTGTCC
AGCCGGATTACATCACGCATCAAAATCATGTCAAATCTTGATATTTCCGAACGACGCATCCCTCAGGACGGGCGCTTTAA
AATGAAGTTATCGCGAACCAGAGCCATAGACTTTCGCGTTAGTACCTGCCCTACTGTCGGTGGAGAAAAAGTAGTAATGC
GTATACTGGATCCGGGTTCAACCAAACTCGGCATCGAAGCGTTAGGATTTAATCCTGATCAAAGGAAACACTTCCTGCAC
GCCATAGAAAAACCCCAGGGAATGATTTTAGTAACCGGCCCGACAGGGAGCGGAAAAACCGTAACGCTTTACACGGCGCT
CAATATCCTGAATACCAAGGAAGCCAACATCTCTACGGCGGAAGATCCGGTAGAGATAAAAGTTCCCGGAATTAATCAAG
TGAATATTAACCCTAAAGCGGGATTAACATTTTCCGGTGCACTTCGATCATTTTTACGCCAGGATCCGGACATTATTATG
GTTGGGGAAATTCGTGATCTTGAGACGGCAGAAATCGCCGTTAAAGCGGCACAGACCGGACATTTGGTTCTGTCAACACT
ACATACCAACAGCGCGGCTGAAACCCTGACCCGCCTGCTGAATATGGGCGTTCCTTCCTTTAACATTGCCAGCTCGGTTA
CTCTGCTCATAGCACAACGACTGGCAAGGCGATTATGCGAAAGTTGCAAGGCACCACGTGATGATTTCACCATTGAAAGC
CTGATGGAGCTGGGGTACAGCAATGAAGAGGCAAAATCATTAAAACCCTATAAAGCGGTTGGATGCAGCCAGTGCACTAA
CGGATATCGAGGGCGTATCGGTTTGTTTGAAGTTCTCCCCATGTCAAAAGCCATAGGGGAATTGATCATGAAAGGCGGCA
ACTCTCTGGACATTCTTAAGCAAGCCCAATCCGAGGGGATGTTAACCATACACGAATCCGGCTTAAAAAAAATTCAGGAA
GGCATGACCACTATTGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(197-464)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0YYT4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

77.526

100

0.775
  pilB Acinetobacter baumannii D1279779

51.494

99.129

0.51
  pilB Acinetobacter baylyi ADP1

49.913

100

0.502
  pilB Vibrio cholerae strain A1552

49.353

94.251

0.465
  pilF Neisseria gonorrhoeae MS11

46.914

98.78

0.463
  pilB Vibrio campbellii strain DS40M4

46.643

98.606

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

47.874

94.251

0.451
  pilF Thermus thermophilus HB27

39.024

100

0.39
  pilB/pilB1 Synechocystis sp. PCC 6803

38.545

95.819

0.369
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.509

92.16

0.364

Multiple sequence alignment