Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   CKV85_RS10460 Genome accession   NZ_LT906454
Coordinates   2124220..2125170 (-) Length   316 a.a.
NCBI ID   WP_269457190.1    Uniprot ID   -
Organism   Streptococcus acidominimus strain NCTC11291     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2119220..2130170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV85_RS10415 (SAMEA4504048_02225) - 2119613..2119810 (-) 198 WP_017770225.1 helix-turn-helix transcriptional regulator -
  CKV85_RS10425 (SAMEA4504048_02227) - 2120200..2121390 (-) 1191 WP_017770223.1 acetate kinase -
  CKV85_RS10430 (SAMEA4504048_02228) comYH 2121451..2122404 (-) 954 WP_095123575.1 class I SAM-dependent methyltransferase Machinery gene
  CKV85_RS10435 (SAMEA4504048_02229) comGG 2122476..2122865 (-) 390 WP_197697097.1 competence type IV pilus minor pilin ComGG -
  CKV85_RS10440 (SAMEA4504048_02230) comYF 2122843..2123280 (-) 438 WP_017770220.1 competence type IV pilus minor pilin ComGF Machinery gene
  CKV85_RS10445 comGE 2123258..2123503 (-) 246 WP_231909805.1 competence system putative prepilin ComGE -
  CKV85_RS10450 (SAMEA4504048_02231) comGD 2123529..2123945 (-) 417 WP_095123577.1 competence type IV pilus minor pilin ComGD -
  CKV85_RS10455 (SAMEA4504048_02232) comYC 2123929..2124219 (-) 291 WP_095123578.1 competence type IV pilus major pilin ComGC Machinery gene
  CKV85_RS10460 (SAMEA4504048_02233) comYB 2124220..2125170 (-) 951 WP_269457190.1 competence type IV pilus assembly protein ComGB Machinery gene
  CKV85_RS10465 (SAMEA4504048_02234) comYA 2125172..2126125 (-) 954 WP_017770215.1 competence type IV pilus ATPase ComGA Machinery gene
  CKV85_RS10470 (SAMEA4504048_02235) - 2126245..2126625 (-) 381 WP_017770214.1 DUF1033 family protein -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 35905.77 Da        Isoelectric Point: 7.0762

>NTDB_id=1147513 CKV85_RS10460 WP_269457190.1 2124220..2125170(-) (comYB) [Streptococcus acidominimus strain NCTC11291]
MIQLFSTLLGSGFNLPETVDFLERSRLVPKQQVSIMKETLLEGFGLPVLMKQLGFSDTVVTQLALAEVHGNQLRSLQKIM
AYLKQVRKVKAKLAEVMTYPIVLLGFLVIIVMGLKNYLLPQLEEQNMATRLLEHFPQIFLGGIFVGLVLVGIAYGLASKM
SSLGLATLLSRLPFVGDLVQLYLTAFYAREWGNLLGQGVEMTNLVQLMQKQESRLFRELGHDMEEAFIAGQSFHEKVMAY
PFFLKELSLIIEYGHAKGHLGQELDIFSEELWERFFKRLNRATQFVQPLVFILVALMIVMIYAAMLLPMYQSMEVM

Nucleotide


Download         Length: 951 bp        

>NTDB_id=1147513 CKV85_RS10460 WP_269457190.1 2124220..2125170(-) (comYB) [Streptococcus acidominimus strain NCTC11291]
TTGATTCAACTTTTTTCGACCTTACTTGGTTCAGGTTTTAACTTGCCAGAGACCGTAGACTTTTTGGAGAGAAGTCGCTT
GGTACCCAAGCAGCAGGTATCTATAATGAAAGAGACCCTCTTAGAGGGTTTTGGCTTGCCGGTCTTGATGAAACAACTTG
GTTTTTCGGATACCGTGGTGACCCAGTTGGCTTTGGCTGAGGTCCATGGCAATCAATTGCGCAGTTTGCAAAAGATTATG
GCCTATCTTAAGCAAGTCAGAAAGGTTAAGGCGAAGTTAGCCGAAGTGATGACATATCCCATTGTCTTGCTGGGTTTTCT
GGTAATCATTGTGATGGGGTTGAAAAACTACTTGCTTCCCCAGCTTGAAGAGCAAAATATGGCGACACGTTTGTTAGAGC
ATTTTCCTCAAATCTTCTTAGGGGGTATCTTTGTTGGTCTTGTCCTGGTAGGCATAGCTTACGGTCTAGCATCCAAGATG
TCTTCGCTAGGCTTAGCTACATTACTGTCTAGATTGCCATTTGTTGGTGACTTGGTTCAGCTCTATCTGACAGCTTTTTA
TGCGCGTGAGTGGGGAAACCTTTTGGGGCAAGGCGTGGAGATGACAAATCTTGTACAACTCATGCAAAAGCAAGAATCAC
GCCTCTTTAGAGAGCTTGGTCACGATATGGAAGAAGCGTTTATCGCGGGTCAATCTTTCCATGAGAAGGTTATGGCTTAC
CCTTTCTTTTTAAAGGAGTTGAGCTTGATTATCGAGTATGGTCACGCCAAGGGACATCTGGGTCAGGAGTTAGATATCTT
TTCTGAGGAGTTGTGGGAGCGATTCTTTAAGCGTCTCAATAGAGCCACTCAGTTTGTGCAACCCCTTGTCTTTATATTGG
TTGCTCTCATGATTGTCATGATTTATGCAGCCATGCTACTACCTATGTATCAATCTATGGAGGTTATGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(188-308)

Type II secretion system (T2SS), protein F (T2SSF)

(2-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

61.27

99.684

0.611
  comYB Streptococcus mutans UA159

60.952

99.684

0.608
  comGB/cglB Streptococcus mitis NCTC 12261

54.574

100

0.547
  comYB Streptococcus gordonii str. Challis substr. CH1

54.603

99.684

0.544
  comGB/cglB Streptococcus pneumoniae Rx1

53.016

99.684

0.528
  comGB/cglB Streptococcus pneumoniae R6

53.016

99.684

0.528
  comGB/cglB Streptococcus pneumoniae TIGR4

53.016

99.684

0.528
  comGB/cglB Streptococcus pneumoniae D39

53.016

99.684

0.528
  comGB/cglB Streptococcus mitis SK321

52.698

99.684

0.525
  comGB Lactococcus lactis subsp. cremoris KW2

47.771

99.367

0.475

Multiple sequence alignment