Detailed information NTG1147511

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: CKV85_RS10440
• Genome accession: NZ_LT906454
• Coordinates: 2122843..2123280 (-)
• Length: 145 a.a.
• NCBI ID: WP_017770220.1
• Uniprot ID: A0A239XJG3
• Organism: Streptococcus acidominimus strain NCTC11291
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2117843..2128280

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKV85_RS10405 (SAMEA4504048_02223)	-	2117952..2118404 (-)	453	WP_095123572.1	hypothetical protein	-
CKV85_RS10410 (SAMEA4504048_02224)	-	2118479..2119483 (+)	1005	WP_095123573.1	IS5 family transposase	-
CKV85_RS10415 (SAMEA4504048_02225)	-	2119613..2119810 (-)	198	WP_017770225.1	helix-turn-helix transcriptional regulator	-
CKV85_RS10425 (SAMEA4504048_02227)	-	2120200..2121390 (-)	1191	WP_017770223.1	acetate kinase	-
CKV85_RS10430 (SAMEA4504048_02228)	comYH	2121451..2122404 (-)	954	WP_095123575.1	class I SAM-dependent methyltransferase	Machinery gene
CKV85_RS10435 (SAMEA4504048_02229)	comGG	2122476..2122865 (-)	390	WP_197697097.1	competence type IV pilus minor pilin ComGG	-
CKV85_RS10440 (SAMEA4504048_02230)	comYF	2122843..2123280 (-)	438	WP_017770220.1	competence type IV pilus minor pilin ComGF	Machinery gene
CKV85_RS10445	comGE	2123258..2123503 (-)	246	WP_231909805.1	competence system putative prepilin ComGE	-
CKV85_RS10450 (SAMEA4504048_02231)	comGD	2123529..2123945 (-)	417	WP_095123577.1	competence type IV pilus minor pilin ComGD	-
CKV85_RS10455 (SAMEA4504048_02232)	comYC	2123929..2124219 (-)	291	WP_095123578.1	competence type IV pilus major pilin ComGC	Machinery gene
CKV85_RS10460 (SAMEA4504048_02233)	comYB	2124220..2125170 (-)	951	WP_269457190.1	competence type IV pilus assembly protein ComGB	Machinery gene
CKV85_RS10465 (SAMEA4504048_02234)	comYA	2125172..2126125 (-)	954	WP_017770215.1	competence type IV pilus ATPase ComGA	Machinery gene
CKV85_RS10470 (SAMEA4504048_02235)	-	2126245..2126625 (-)	381	WP_017770214.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16970.63 Da
• Isoelectric Point: 9.8697

>NTDB_id=1147511 CKV85_RS10440 WP_017770220.1 2122843..2123280(-) (comYF) [Streptococcus acidominimus strain NCTC11291]
MLKSYRLRAFTLMECLIALFVLTGSVQVYQAMTKVISQQVHHLQKDRESDWLLFCQQLRYELSESRLSKVTKDKLYVVKD
TKPLAFGKSKADDFRKTSAGGRGYQPMLYDLASATFTQFDHTVSARFEFKDGKVREFLYVFEKER

Nucleotide

• Length: 438 bp

>NTDB_id=1147511 CKV85_RS10440 WP_017770220.1 2122843..2123280(-) (comYF) [Streptococcus acidominimus strain NCTC11291]
GTGTTAAAGTCTTATCGCCTTAGAGCTTTTACATTAATGGAATGTTTGATTGCTTTGTTCGTCTTAACAGGATCTGTTCA
GGTTTATCAAGCTATGACTAAGGTGATCTCACAGCAAGTTCATCATTTACAAAAGGATCGTGAGAGCGATTGGTTACTCT
TTTGTCAGCAATTACGTTATGAGTTATCTGAATCCCGTTTAAGCAAGGTGACTAAGGATAAATTGTATGTTGTCAAAGAT
ACCAAGCCTTTAGCCTTTGGGAAATCTAAAGCAGATGATTTTCGTAAGACAAGTGCAGGAGGGCGTGGTTATCAGCCGAT
GCTTTATGATTTAGCTTCAGCGACGTTCACGCAGTTTGACCATACTGTCAGCGCTCGTTTTGAATTTAAAGATGGAAAGG
TGCGAGAATTTCTTTATGTCTTTGAAAAGGAACGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (36-134)

3D structure

• AlphaFold DB: A0A239XJG3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	53.103	100	0.531
comYF	Streptococcus mutans UA159	52.414	100	0.524
comGF/cglF	Streptococcus mitis NCTC 12261	48.252	98.621	0.476
comGF/cglF	Streptococcus pneumoniae TIGR4	46.154	98.621	0.455
comGF/cglF	Streptococcus pneumoniae D39	46.154	98.621	0.455
comGF/cglF	Streptococcus pneumoniae Rx1	46.154	98.621	0.455
comGF/cglF	Streptococcus pneumoniae R6	46.154	98.621	0.455
comGF/cglF	Streptococcus mitis SK321	45.455	98.621	0.448
comGF	Lactococcus lactis subsp. cremoris KW2	44.853	93.793	0.421