Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   CKV66_RS04115 Genome accession   NZ_LT906434
Coordinates   901827..903065 (+) Length   412 a.a.
NCBI ID   WP_085364434.1    Uniprot ID   A0AB38DQ02
Organism   Neisseria zoodegmatis strain NCTC12230     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 896827..908065
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV66_RS12510 - 898122..898303 (+) 182 Protein_798 lipoprotein signal peptidase -
  CKV66_RS04100 (SAMEA4504057_00805) - 898406..899038 (-) 633 WP_085364432.1 isochorismatase family protein -
  CKV66_RS04110 (SAMEA4504057_00807) pilF 900117..901796 (+) 1680 WP_085364433.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKV66_RS04115 (SAMEA4504057_00808) pilG 901827..903065 (+) 1239 WP_085364434.1 type II secretion system F family protein Machinery gene
  CKV66_RS04120 (SAMEA4504057_00809) pilD 903065..903931 (+) 867 WP_408633884.1 prepilin peptidase Machinery gene
  CKV66_RS04125 (SAMEA4504057_00810) coaE 903935..904558 (+) 624 WP_085364436.1 dephospho-CoA kinase -
  CKV66_RS04130 (SAMEA4504057_00811) zapD 904555..905325 (+) 771 WP_085364437.1 cell division protein ZapD -
  CKV66_RS04135 (SAMEA4504057_00812) yacG 905329..905511 (+) 183 WP_085364438.1 DNA gyrase inhibitor YacG -
  CKV66_RS04145 (SAMEA4504057_00814) ubiD 905960..907438 (+) 1479 WP_085364439.1 4-hydroxy-3-polyprenylbenzoate decarboxylase -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 45370.54 Da        Isoelectric Point: 10.0019

>NTDB_id=1146934 CKV66_RS04115 WP_085364434.1 901827..903065(+) (pilG) [Neisseria zoodegmatis strain NCTC12230]
MSRSVAGSRNVKKEKGIRFNFEGRNIDTEQMVRGEVVAKNEEEARKKLQRRGIKPLRVSKVKAVRKKRITQEDITVFTRQ
LATMMKAGLPLMQAFEIVAKGHSNPSMTQMLMQVRADVEQGSALGKAFAKHPKYFDRFYCNLIAAGETGGVLESLLDKLA
VYKEKTQAIKKKVKSALTYPIAIVVVAVVLVFIMMVFVLPAFGEVYSSMGAELPGLTQIVMSISDFFVKYAWVIVIGTVA
LCFGVYQWYKKSPVLQKRVDAMMLRLPIFGSIVRKATIARWARTTSTLFAAGVPLVEVLDSVSGAAGNILYEEATHDIRA
KVTQGLSLTSSMQGTDMFPNMVLQMAAIGEESGSLDDMLNKAAEFYEDEVDNAVAQLSSLMEPIIMVVLGSIIGIILVAM
YLPLFNLGNVVG

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=1146934 CKV66_RS04115 WP_085364434.1 901827..903065(+) (pilG) [Neisseria zoodegmatis strain NCTC12230]
ATGAGCAGGTCTGTAGCAGGAAGTAGAAACGTTAAAAAAGAAAAAGGCATACGTTTTAACTTTGAAGGCAGAAACATCGA
CACCGAACAAATGGTTCGTGGCGAAGTTGTTGCCAAAAACGAAGAAGAGGCACGTAAAAAGCTTCAACGTCGGGGTATTA
AGCCGCTTCGTGTCAGCAAAGTTAAGGCTGTACGCAAGAAAAGAATTACGCAGGAAGACATCACCGTATTTACCCGCCAA
CTGGCTACCATGATGAAGGCAGGGTTGCCGTTGATGCAAGCGTTTGAAATTGTTGCGAAGGGTCACTCCAACCCGTCAAT
GACGCAAATGCTCATGCAGGTGCGTGCCGATGTAGAACAGGGTAGTGCCTTAGGTAAGGCTTTTGCCAAACATCCTAAAT
ATTTCGACCGCTTCTATTGCAACCTTATTGCAGCGGGTGAGACAGGTGGTGTGTTGGAAAGCCTGCTTGATAAATTGGCT
GTTTATAAAGAAAAAACCCAAGCAATTAAGAAAAAGGTTAAAAGTGCGCTGACTTATCCGATTGCGATCGTTGTCGTTGC
CGTTGTATTGGTTTTCATTATGATGGTGTTTGTATTGCCGGCTTTCGGTGAAGTGTACAGCAGCATGGGTGCAGAGCTGC
CGGGGTTGACTCAAATTGTGATGAGTATTTCCGACTTCTTTGTTAAATATGCGTGGGTTATTGTGATTGGAACGGTAGCA
CTGTGCTTTGGTGTTTACCAGTGGTACAAAAAATCACCAGTGTTGCAAAAACGTGTTGATGCCATGATGCTCAGATTGCC
TATTTTTGGTTCGATTGTCAGAAAAGCGACCATCGCACGTTGGGCACGTACAACTTCTACTCTGTTTGCAGCCGGTGTAC
CTTTAGTGGAAGTGTTGGATTCCGTATCAGGCGCGGCGGGCAATATCCTTTATGAAGAAGCTACCCATGATATTCGAGCC
AAAGTTACTCAAGGTTTGTCATTGACATCCAGCATGCAGGGCACAGATATGTTCCCCAATATGGTGTTGCAAATGGCCGC
TATCGGTGAAGAATCAGGTTCGCTGGATGATATGTTGAATAAGGCCGCAGAGTTTTATGAAGACGAAGTGGATAATGCCG
TAGCACAGCTTTCATCATTGATGGAACCGATTATTATGGTTGTGCTGGGTTCCATCATCGGCATTATCTTGGTCGCCATG
TATCTGCCGTTGTTCAACTTGGGTAATGTGGTAGGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(77-200)

Type II secretion system (T2SS), protein F (T2SSF)

(281-403)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

69.082

100

0.694
  pilG Neisseria meningitidis 44/76-A

68.599

100

0.689
  pilC Legionella pneumophila strain ERS1305867

47.761

97.573

0.466
  pilC Acinetobacter baumannii D1279779

44.862

96.845

0.434
  pilC Acinetobacter baylyi ADP1

44.612

96.845

0.432
  pilC Pseudomonas stutzeri DSM 10701

44.836

96.359

0.432
  pilC Vibrio cholerae strain A1552

38.693

96.602

0.374
  pilC Vibrio campbellii strain DS40M4

37.688

96.602

0.364

Multiple sequence alignment