Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CKV66_RS04110 Genome accession   NZ_LT906434
Coordinates   900117..901796 (+) Length   559 a.a.
NCBI ID   WP_085364433.1    Uniprot ID   A0AB38DQR6
Organism   Neisseria zoodegmatis strain NCTC12230     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 895117..906796
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV66_RS12510 - 898122..898303 (+) 182 Protein_798 lipoprotein signal peptidase -
  CKV66_RS04100 (SAMEA4504057_00805) - 898406..899038 (-) 633 WP_085364432.1 isochorismatase family protein -
  CKV66_RS04110 (SAMEA4504057_00807) pilF 900117..901796 (+) 1680 WP_085364433.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CKV66_RS04115 (SAMEA4504057_00808) pilG 901827..903065 (+) 1239 WP_085364434.1 type II secretion system F family protein Machinery gene
  CKV66_RS04120 (SAMEA4504057_00809) pilD 903065..903931 (+) 867 WP_408633884.1 prepilin peptidase Machinery gene
  CKV66_RS04125 (SAMEA4504057_00810) coaE 903935..904558 (+) 624 WP_085364436.1 dephospho-CoA kinase -
  CKV66_RS04130 (SAMEA4504057_00811) zapD 904555..905325 (+) 771 WP_085364437.1 cell division protein ZapD -
  CKV66_RS04135 (SAMEA4504057_00812) yacG 905329..905511 (+) 183 WP_085364438.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62379.04 Da        Isoelectric Point: 6.5094

>NTDB_id=1146933 CKV66_RS04110 WP_085364433.1 900117..901796(+) (pilF) [Neisseria zoodegmatis strain NCTC12230]
MSVGILRVLVQSQVIDNNQVERYQNVLKANKPILPLLFEEGIITPKALGELLARVFSYPLLDLTHYPRNNILSDVLSEEQ
MLQNRCIPIFQRGRKVYFAVSDPTQIQNFQKIAFSSGVSVDLVVVRDDQLSTLLEWLGQRSTSILNEMSKEQEAAAPVQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDAGSLNIDQLGFEPFQKEMLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTENVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKAFLRQDPDIIMVGEIRDLETADISIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCPHCKVPMERPPLPALKKAGFTDEDLAKNWTM
YRPVGCDSCRGKGYKGRAGVYEVMPITEEMQRVIMENGTEVDILNMAYKEGMVDLRRAGLLKVMQGITSLEEVLAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=1146933 CKV66_RS04110 WP_085364433.1 900117..901796(+) (pilF) [Neisseria zoodegmatis strain NCTC12230]
ATGAGCGTCGGTATATTAAGAGTATTGGTGCAGAGCCAAGTCATCGACAATAATCAGGTCGAACGGTATCAAAACGTATT
AAAAGCCAATAAACCTATTCTCCCTCTTTTGTTTGAAGAGGGCATTATCACACCTAAAGCATTAGGGGAATTGCTTGCCA
GAGTATTCAGCTATCCTCTGCTGGATTTAACCCACTATCCCCGCAACAACATCCTTTCTGACGTGCTCTCGGAAGAGCAA
ATGCTGCAAAACCGCTGTATTCCGATTTTTCAAAGAGGTCGTAAAGTTTATTTTGCAGTTTCAGATCCCACACAAATTCA
AAATTTTCAAAAAATAGCTTTTTCATCAGGCGTATCAGTTGACTTGGTTGTCGTGCGTGACGACCAACTAAGCACGTTAT
TGGAATGGCTGGGTCAACGCTCCACTTCGATTCTGAATGAGATGAGCAAAGAGCAGGAAGCAGCGGCACCTGTTCAATCT
CTGTATATCGATAATGAAGAAGCAGAAGACGGGCCTATCCCACGTTTTATTCATAAAACACTCTCTGATGCCTTGAACGC
TGGTGCTTCGGATATCCACTTTGAGTTTTACGAGCAAATGGCTCGGGTTCGTTTCCGCGTTGACGGTCAGCTACGCGAAG
TAGTACAACCACCCGTAGCTGTACGCGGCCAGCTTGCATCTCGCATTAAGGTTATGGCGCGCTTGGACATTTCTGAAAAA
CGCGTACCTCAAGACGGCCGTATTCAGATTGCCTTCCACAAACATGGCCGCCCGATCGACTTCCGTGTCAGCACCCTACC
GACATTGTTCGGTGAAAAAGTGGTGATGCGTATTCTGAATTCAGATGCGGGTTCGTTAAATATCGACCAACTGGGCTTCG
AGCCGTTCCAAAAAGAAATGCTGCTGGAAGCCATCCACCGTCCTTACGGCATGGTGTTGGTAACGGGTCCCACTGGCTCG
GGTAAAACGGTGTCGCTTTATACCTGTTTGAACATCTTAAATACGGAAAATGTCAATATCGCCACGGCAGAAGACCCTGC
CGAGATCAATTTACCCGGAATTAATCAGGTAAACGTAAACGACAAACAGGGCTTGACCTTTGCAGCGGCTCTTAAAGCTT
TCCTCCGTCAAGATCCGGATATCATCATGGTGGGTGAGATTCGTGACTTGGAAACTGCCGATATCTCCATTAAAGCCGCA
CAAACCGGTCACATGGTGTTCTCTACGCTGCACACTAACAACGCACCGGCAACTTTGTCGCGTATGCTGAACATGGGCGT
TGCACCGTTTAATATCGCCAGCTCTGTGAGCTTGATTATGGCTCAGCGTCTGTTACGGCGCTTGTGTCCGCATTGCAAAG
TTCCTATGGAACGTCCGCCTCTGCCTGCTTTGAAGAAAGCTGGTTTTACCGATGAAGACTTGGCAAAAAATTGGACTATG
TACCGTCCTGTAGGCTGCGACAGCTGTCGAGGGAAAGGATATAAAGGACGTGCCGGTGTCTATGAAGTGATGCCTATCAC
CGAAGAAATGCAGCGGGTCATCATGGAGAATGGTACGGAAGTCGACATTTTAAATATGGCTTACAAAGAAGGCATGGTCG
ATTTGCGCCGTGCGGGCTTGTTGAAAGTCATGCAGGGTATCACTTCATTGGAAGAAGTGTTGGCTCATACTAACGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

78.175

100

0.782
  pilB Acinetobacter baumannii D1279779

51.346

99.642

0.512
  pilB Acinetobacter baylyi ADP1

51.167

99.642

0.51
  pilB Legionella pneumophila strain ERS1305867

47.331

100

0.476
  pilB Vibrio cholerae strain A1552

45.567

100

0.46
  pilB Vibrio parahaemolyticus RIMD 2210633

45.196

100

0.454
  pilB Vibrio campbellii strain DS40M4

43.604

99.284

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.815

93.56

0.363
  pilF Thermus thermophilus HB27

38.258

94.454

0.361

Multiple sequence alignment