Detailed information NTG1145335

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DXE33_RS03900
• Genome accession: NZ_LT615228
• Coordinates: 747402..748226 (-)
• Length: 274 a.a.
• NCBI ID: WP_269460035.1
• Uniprot ID: -
• Organism: Polynucleobacter necessarius isolate PPGSP1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 742402..753226

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DXE33_RS03875	-	742512..743897 (+)	1386	WP_114638693.1	phosphomannomutase/phosphoglucomutase	-
DXE33_RS03885	-	743894..744936 (-)	1043	Protein_797	cyclic nucleotide-binding domain-containing protein	-
DXE33_RS03890	-	745335..745958 (-)	624	WP_197711989.1	lytic transglycosylase domain-containing protein	-
DXE33_RS03895	-	745963..747210 (-)	1248	WP_114638696.1	type II secretion system F family protein	-
DXE33_RS09595	-	747226..747396 (+)	171	WP_162785399.1	hypothetical protein	-
DXE33_RS03900	pilB	747402..748226 (-)	825	WP_269460035.1	GspE/PulE family protein	Machinery gene
DXE33_RS03905	-	748260..748910 (-)	651	WP_162785400.1	hypothetical protein	-
DXE33_RS10380	-	748920..749042 (-)	123	WP_269460018.1	hypothetical protein	-
DXE33_RS03910	pilQ	749045..750493 (-)	1449	WP_114638699.1	type IV pilus secretin PilQ	-
DXE33_RS03915	-	750607..751845 (-)	1239	WP_114638700.1	hypothetical protein	-

Sequence

Protein

• Length: 274 a.a.
• Molecular weight: 29837.38 Da
• Isoelectric Point: 6.7489

>NTDB_id=1145335 DXE33_RS03900 WP_269460035.1 747402..748226(-) (pilB) [Polynucleobacter necessarius isolate PPGSP1]
MTTRIKIMASLDISERRLPQDGAIVTSLPNNRDIDLRVSVLPTVFGERVVMRILDREGVSFDLDKLGFPEKEFKDVTSAI
DASQGMVLVTGPTGSGKSTTLYGALRRLNKPDINILTAEDPVEFTVEGIGQVQIRDDIGLTFSQALRSFLRQDPEVILVG
EVRVKDTADISIKASLTGHLVLSTLHANDAISTIVRLVNMGIPGYLIGAALTLVIAQRLARKICTHCKQEDHGDHLASLL
DIGFTSEEAANLKLYVGKGCEKCNHTGYKGRQGI

Nucleotide

• Length: 825 bp

>NTDB_id=1145335 DXE33_RS03900 WP_269460035.1 747402..748226(-) (pilB) [Polynucleobacter necessarius isolate PPGSP1]
ATCACGACGCGCATAAAAATTATGGCCTCCTTGGATATTTCCGAGAGACGCTTGCCGCAAGACGGCGCAATCGTGACGTC
TTTACCAAACAATCGTGATATTGATTTACGCGTTTCCGTACTGCCTACGGTGTTTGGAGAGCGTGTAGTAATGCGTATCT
TGGATCGGGAAGGAGTTTCTTTTGACCTTGATAAATTAGGCTTTCCTGAAAAAGAGTTTAAGGATGTTACCTCTGCGATT
GATGCATCTCAGGGAATGGTGTTGGTTACTGGCCCTACTGGCTCGGGTAAATCAACAACGCTTTATGGTGCTTTAAGAAG
ATTAAACAAGCCTGACATCAATATTCTGACTGCGGAAGATCCGGTCGAATTTACGGTAGAAGGTATTGGGCAAGTCCAAA
TTCGTGACGATATTGGCTTAACCTTTTCTCAAGCACTGCGCTCATTTTTGCGCCAAGACCCAGAGGTTATATTGGTGGGC
GAGGTACGCGTTAAGGATACCGCCGACATCTCGATTAAAGCGTCTCTTACGGGCCATCTAGTTCTTTCGACTTTGCATGC
GAATGATGCGATTAGTACCATTGTCCGTTTAGTAAATATGGGTATTCCAGGCTACTTGATTGGTGCAGCCCTTACTTTAG
TGATTGCGCAGCGCTTGGCTAGAAAGATTTGCACGCATTGCAAACAAGAAGATCATGGTGATCATCTGGCTAGCTTATTA
GATATTGGATTTACCTCTGAAGAGGCTGCAAACCTCAAGCTATATGTCGGCAAAGGCTGTGAAAAATGTAACCACACGGG
TTACAAGGGGCGGCAGGGTATTTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (2-222)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Legionella pneumophila strain ERS1305867	54.71	100	0.551
pilB	Vibrio parahaemolyticus RIMD 2210633	53.65	100	0.537
pilB	Vibrio campbellii strain DS40M4	51.986	100	0.526
pilF	Neisseria gonorrhoeae MS11	50.725	100	0.511
pilB	Acinetobacter baumannii D1279779	49.638	100	0.5
pilB	Vibrio cholerae strain A1552	50	100	0.5
pilB	Acinetobacter baylyi ADP1	48.913	100	0.493
pilF	Thermus thermophilus HB27	48.519	98.54	0.478
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	47.81	100	0.478
pilB/pilB1	Synechocystis sp. PCC 6803	45.139	100	0.474
pilB	Glaesserella parasuis strain SC1401	45.985	100	0.46
pilB	Haemophilus influenzae Rd KW20	45.956	99.27	0.456
pilB	Haemophilus influenzae 86-028NP	45.956	99.27	0.456
ctsE	Campylobacter jejuni subsp. jejuni 81-176	44.161	100	0.442