Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DQL17_RS03420 Genome accession   NZ_LS483496
Coordinates   670619..672013 (-) Length   464 a.a.
NCBI ID   WP_005691826.1    Uniprot ID   A0A2S9S146
Organism   Haemophilus influenzae strain NCTC8455     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 665619..677013
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL17_RS03385 (NCTC8455_00666) - 665868..666074 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  DQL17_RS03390 (NCTC8455_00667) - 666149..666355 (-) 207 WP_005648955.1 heavy-metal-associated domain-containing protein -
  DQL17_RS03395 (NCTC8455_00668) cueR 666432..666818 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  DQL17_RS03400 (NCTC8455_00669) metJ 666832..667149 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  DQL17_RS03405 (NCTC8455_00670) rho 667397..668659 (+) 1263 WP_111688882.1 transcription termination factor Rho -
  DQL17_RS03410 (NCTC8455_00671) pilD 668713..669405 (-) 693 WP_111688883.1 prepilin peptidase Machinery gene
  DQL17_RS03415 (NCTC8455_00672) pilC 669402..670622 (-) 1221 WP_005691827.1 type II secretion system F family protein Machinery gene
  DQL17_RS03420 (NCTC8455_00673) pilB 670619..672013 (-) 1395 WP_005691826.1 GspE/PulE family protein Machinery gene
  DQL17_RS03425 (NCTC8455_00674) pilA 672010..672459 (-) 450 WP_005691824.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DQL17_RS03430 (NCTC8455_00675) ampD 672574..673128 (+) 555 WP_005691822.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DQL17_RS03435 (NCTC8455_00676) corC 673760..674659 (+) 900 WP_005654105.1 CNNM family magnesium/cobalt transport protein CorC -
  DQL17_RS03440 (NCTC8455_00677) lnt 674682..676211 (+) 1530 WP_080003747.1 apolipoprotein N-acyltransferase -
  DQL17_RS03445 (NCTC8455_00678) rsmE 676261..676998 (+) 738 WP_005691816.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52953.39 Da        Isoelectric Point: 6.2919

>NTDB_id=1142727 DQL17_RS03420 WP_005691826.1 670619..672013(-) (pilB) [Haemophilus influenzae strain NCTC8455]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALLLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPRQMQVEEQVKFYQHQETHFEQENDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIVQSQINPQIGLDFNRLLR
TFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKLCSKC
GGNLANSCDCHQGYRGRIGVYQFLHWQQNGYQTDFKNLRASGLEKVNQGITDEKELERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1142727 DQL17_RS03420 WP_005691826.1 670619..672013(-) (pilB) [Haemophilus influenzae strain NCTC8455]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAACAACAATCCTTGCTCTTACGTTATTTTGCTTTGCTACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCGTCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAAATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGGTTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACAGCGGAAGATC
CCATTGAAATTGAGCTGGATGGCATTGTTCAAAGCCAAATTAATCCACAGATTGGACTAGATTTTAACCGCCTATTGCGC
ACTTTCTTACGCCAAGATCCCGATATTATTATGTTAGGCGAAATTCGCGATGAAGAAAGTGCAAGGATTGCATTACGTGC
GGCACAAACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAGCTTTGTTCAAAGTGC
GGTGGAAATTTAGCAAATTCTTGTGATTGCCATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACGTGCGAGTGGTTTAGAAAAAGTTAATCAAGGAATAACAGATGAAA
AAGAACTTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-97)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2S9S146

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

97.192

99.784

0.97
  pilB Haemophilus influenzae 86-028NP

96.336

100

0.963
  pilB Glaesserella parasuis strain SC1401

57.33

98.491

0.565
  pilB Vibrio cholerae strain A1552

39.658

100

0.45
  pilB Vibrio campbellii strain DS40M4

40.695

100

0.429
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.351

100

0.418
  pilB Acinetobacter baylyi ADP1

38.911

100

0.416
  pilB Acinetobacter baumannii D1279779

39.501

100

0.409
  pilF Neisseria gonorrhoeae MS11

37.395

100

0.384
  pilF Thermus thermophilus HB27

36.515

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.179

100

0.375

Multiple sequence alignment