Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DQL18_RS04015 Genome accession   NZ_LS483486
Coordinates   762457..763851 (+) Length   464 a.a.
NCBI ID   WP_105905461.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC12194     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 757457..768851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL18_RS03990 (NCTC12194_00806) rsmE 757476..758213 (-) 738 WP_011271976.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DQL18_RS03995 (NCTC12194_00807) lnt 758263..759792 (-) 1530 WP_079989936.1 apolipoprotein N-acyltransferase -
  DQL18_RS04000 (NCTC12194_00808) corC 759815..760714 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  DQL18_RS04005 (NCTC12194_00809) ampD 761336..761896 (-) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DQL18_RS04010 (NCTC12194_00810) pilA 762011..762460 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DQL18_RS04015 (NCTC12194_00811) pilB 762457..763851 (+) 1395 WP_105905461.1 GspE/PulE family protein Machinery gene
  DQL18_RS04020 (NCTC12194_00812) pilC 763848..765068 (+) 1221 WP_042601844.1 type II secretion system F family protein Machinery gene
  DQL18_RS04025 (NCTC12194_00813) pilD 765065..765757 (+) 693 WP_105905460.1 prepilin peptidase Machinery gene
  DQL18_RS04030 (NCTC12194_00814) rho 765811..767073 (-) 1263 WP_005648966.1 transcription termination factor Rho -
  DQL18_RS04035 (NCTC12194_00815) metJ 767321..767638 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  DQL18_RS04040 (NCTC12194_00816) cueR 767652..768038 (-) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  DQL18_RS04045 (NCTC12194_00817) - 768115..768321 (+) 207 WP_048941374.1 heavy-metal-associated domain-containing protein -
  DQL18_RS04050 (NCTC12194_00818) - 768395..768601 (+) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52978.30 Da        Isoelectric Point: 5.4806

>NTDB_id=1142630 DQL18_RS04015 WP_105905461.1 762457..763851(+) (pilB) [Haemophilus influenzae strain NCTC12194]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLCSKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1142630 DQL18_RS04015 WP_105905461.1 762457..763851(+) (pilB) [Haemophilus influenzae strain NCTC12194]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGTTCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

99.353

100

0.994
  pilB Haemophilus influenzae Rd KW20

97.192

99.784

0.97
  pilB Glaesserella parasuis strain SC1401

57.895

98.276

0.569
  pilB Vibrio cholerae strain A1552

39.848

100

0.453
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.418
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Acinetobacter baylyi ADP1

38.508

100

0.412
  pilB Acinetobacter baumannii D1279779

39.085

100

0.405
  pilF Neisseria gonorrhoeae MS11

36.975

100

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.607

100

0.379
  pilF Thermus thermophilus HB27

36.134

100

0.371

Multiple sequence alignment