Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DQN75_RS00660 Genome accession   NZ_LS483420
Coordinates   101669..102607 (+) Length   312 a.a.
NCBI ID   WP_111684478.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC13739     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 96669..107607
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN75_RS00650 (NCTC13739_00132) rpoC 97417..101058 (+) 3642 WP_032460334.1 DNA-directed RNA polymerase subunit beta' -
  DQN75_RS00655 (NCTC13739_00133) - 101211..101576 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQN75_RS00660 (NCTC13739_00134) comYA 101669..102607 (+) 939 WP_111684478.1 competence type IV pilus ATPase ComGA Machinery gene
  DQN75_RS00665 (NCTC13739_00135) comYB 102543..103577 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQN75_RS00670 (NCTC13739_00136) comYC 103579..103905 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DQN75_RS00675 (NCTC13739_00137) comGD 103880..104308 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQN75_RS00680 (NCTC13739_00138) comGE 104265..104549 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  DQN75_RS00685 (NCTC13739_00139) comYF 104542..104976 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQN75_RS00690 (NCTC13739_00140) comGG 104960..105286 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  DQN75_RS00695 (NCTC13739_00141) comYH 105384..106337 (+) 954 WP_111684480.1 class I SAM-dependent methyltransferase Machinery gene
  DQN75_RS00700 (NCTC13739_00142) - 106396..107592 (+) 1197 WP_111684482.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35078.35 Da        Isoelectric Point: 9.1037

>NTDB_id=1140979 DQN75_RS00660 WP_111684478.1 101669..102607(+) (comYA) [Streptococcus pyogenes strain NCTC13739]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLVSHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQKA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLFEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1140979 DQN75_RS00660 WP_111684478.1 101669..102607(+) (comYA) [Streptococcus pyogenes strain NCTC13739]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTGTTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTCAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAGGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAAAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTTTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.129

99.679

0.599
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60

99.359

0.596
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.199

99.679

0.58
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.199

99.679

0.58
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.655

92.949

0.369

Multiple sequence alignment