Detailed information NTG1140982

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQN75_RS00685
• Genome accession: NZ_LS483420
• Coordinates: 104542..104976 (+)
• Length: 144 a.a.
• NCBI ID: WP_002992738.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC13739
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99542..109976

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQN75_RS00655 (NCTC13739_00133)	-	101211..101576 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQN75_RS00660 (NCTC13739_00134)	comYA	101669..102607 (+)	939	WP_111684478.1	competence type IV pilus ATPase ComGA	Machinery gene
DQN75_RS00665 (NCTC13739_00135)	comYB	102543..103577 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQN75_RS00670 (NCTC13739_00136)	comYC	103579..103905 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
DQN75_RS00675 (NCTC13739_00137)	comGD	103880..104308 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
DQN75_RS00680 (NCTC13739_00138)	comGE	104265..104549 (+)	285	WP_011284422.1	competence type IV pilus minor pilin ComGE	-
DQN75_RS00685 (NCTC13739_00139)	comYF	104542..104976 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQN75_RS00690 (NCTC13739_00140)	comGG	104960..105286 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
DQN75_RS00695 (NCTC13739_00141)	comYH	105384..106337 (+)	954	WP_111684480.1	class I SAM-dependent methyltransferase	Machinery gene
DQN75_RS00700 (NCTC13739_00142)	-	106396..107592 (+)	1197	WP_111684482.1	acetate kinase	-
DQN75_RS00705	-	107778..108086 (+)	309	WP_030126458.1	hypothetical protein	-
DQN75_RS00710 (NCTC13739_00143)	proC	108169..108939 (-)	771	WP_002992745.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16645.27 Da
• Isoelectric Point: 10.2526

>NTDB_id=1140982 DQN75_RS00685 WP_002992738.1 104542..104976(+) (comYF) [Streptococcus pyogenes strain NCTC13739]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1140982 DQN75_RS00685 WP_002992738.1 104542..104976(+) (comYF) [Streptococcus pyogenes strain NCTC13739]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	46.715	95.139	0.444
comGF/cglF	Streptococcus mitis NCTC 12261	44.776	93.056	0.417
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396