Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DQM65_RS03795 Genome accession   NZ_LS483392
Coordinates   733265..734659 (-) Length   464 a.a.
NCBI ID   WP_111691284.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC11931     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 728265..739659
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM65_RS03760 (NCTC11931_00750) - 728515..728721 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  DQM65_RS03765 (NCTC11931_00751) - 728796..729002 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  DQM65_RS03770 (NCTC11931_00752) cueR 729079..729465 (+) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  DQM65_RS03775 (NCTC11931_00753) metJ 729479..729796 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  DQM65_RS03780 (NCTC11931_00754) rho 730043..731305 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  DQM65_RS03785 (NCTC11931_00755) pilD 731359..732051 (-) 693 WP_111691282.1 A24 family peptidase Machinery gene
  DQM65_RS03790 (NCTC11931_00756) pilC 732048..733268 (-) 1221 WP_111691283.1 type II secretion system F family protein Machinery gene
  DQM65_RS03795 (NCTC11931_00757) pilB 733265..734659 (-) 1395 WP_111691284.1 GspE/PulE family protein Machinery gene
  DQM65_RS03800 (NCTC11931_00758) pilA 734656..735105 (-) 450 WP_111691285.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DQM65_RS03805 (NCTC11931_00759) ampD 735220..735774 (+) 555 WP_111691286.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  DQM65_RS03810 (NCTC11931_00760) corC 736405..737304 (+) 900 WP_111691287.1 CNNM family magnesium/cobalt transport protein CorC -
  DQM65_RS03815 (NCTC11931_00761) lnt 737327..738856 (+) 1530 WP_111691987.1 apolipoprotein N-acyltransferase -
  DQM65_RS03820 (NCTC11931_00762) rsmE 738906..739643 (+) 738 WP_111691288.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52870.40 Da        Isoelectric Point: 5.6568

>NTDB_id=1140040 DQM65_RS03795 WP_111691284.1 733265..734659(-) (pilB) [Haemophilus influenzae strain NCTC11931]
MTSYALLHTQRVTAKNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFTNRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1140040 DQM65_RS03795 WP_111691284.1 733265..734659(-) (pilB) [Haemophilus influenzae strain NCTC11931]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTAAAAATGGCGAAGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTACGTTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCCTGTGAAACCATTGCGTTTATAACGGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGTGCCAAATGCAAGTGGAAGAACAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCACCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATAGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAGAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAATGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGATATTATTATGCTGGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAGACTGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGTTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAAATTTATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-95)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

97.414

100

0.974
  pilB Haemophilus influenzae 86-028NP

97.408

99.784

0.972
  pilB Glaesserella parasuis strain SC1401

57.675

98.276

0.567
  pilB Vibrio cholerae strain A1552

39.006

100

0.44
  pilB Vibrio campbellii strain DS40M4

40.286

100

0.425
  pilB Vibrio parahaemolyticus RIMD 2210633

40.166

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.148

100

0.416
  pilB Acinetobacter baylyi ADP1

37.903

100

0.405
  pilB Acinetobacter baumannii D1279779

38.351

100

0.401
  pilF Neisseria gonorrhoeae MS11

36.345

100

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.966

100

0.373
  pilF Thermus thermophilus HB27

35.924

100

0.369

Multiple sequence alignment