Detailed information NTG1139653

Bioinformatically predicted

Overview

• Name: comGF/cglF
• Type: Machinery gene
• Locus tag: DQM63_RS10535
• Genome accession: NZ_LS483385
• Coordinates: 2114250..2114687 (-)
• Length: 145 a.a.
• NCBI ID: WP_002901713.1
• Uniprot ID: F2CFN3
• Organism: Streptococcus sanguinis strain NCTC7863
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2109250..2119687

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQM63_RS10510 (NCTC7863_02074)	-	2109963..2110601 (-)	639	WP_002901701.1	hypothetical protein	-
DQM63_RS10515 (NCTC7863_02075)	-	2110719..2111468 (-)	750	WP_032909647.1	CPBP family intramembrane glutamic endopeptidase	-
DQM63_RS10520 (NCTC7863_02076)	-	2111603..2112796 (-)	1194	WP_002901705.1	acetate kinase	-
DQM63_RS10525 (NCTC7863_02077)	comYH	2112833..2113798 (-)	966	WP_002901707.1	class I SAM-dependent methyltransferase	Machinery gene
DQM63_RS10530 (NCTC7863_02078)	comGG	2113880..2114269 (-)	390	WP_002901710.1	competence type IV pilus minor pilin ComGG	-
DQM63_RS10535 (NCTC7863_02079)	comGF/cglF	2114250..2114687 (-)	438	WP_002901713.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQM63_RS10540 (NCTC7863_02080)	comGE/cglE	2114671..2114964 (-)	294	WP_002914478.1	competence type IV pilus minor pilin ComGE	Machinery gene
DQM63_RS10545 (NCTC7863_02081)	comYD	2114930..2115364 (-)	435	WP_002901717.1	competence type IV pilus minor pilin ComGD	Machinery gene
DQM63_RS10550 (NCTC7863_02082)	comYC	2115324..2115641 (-)	318	WP_002896769.1	competence type IV pilus major pilin ComGC	Machinery gene
DQM63_RS10555 (NCTC7863_02083)	comYB	2115641..2116675 (-)	1035	WP_263281106.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQM63_RS10560 (NCTC7863_02084)	comYA	2116605..2117546 (-)	942	WP_002901721.1	competence type IV pilus ATPase ComGA	Machinery gene
DQM63_RS10565 (NCTC7863_02085)	-	2117676..2118056 (-)	381	WP_002901723.1	DUF1033 family protein	-
DQM63_RS10575 (NCTC7863_02086)	-	2118375..2119481 (-)	1107	WP_002901727.1	glycosyl hydrolase family 8	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16769.00 Da
• Isoelectric Point: 5.3160

>NTDB_id=1139653 DQM63_RS10535 WP_002901713.1 2114250..2114687(-) (comGF/cglF) [Streptococcus sanguinis strain NCTC7863]
MSKNLKVKAFTLLETLVALLVLSGGVLVFQAMTQLLSSELHQQENNQQQEWLLFADQLETELSRSQFDKVEDNKIYIRQD
GRDLALGKSKGDDFRKTDKSGRGYQPMIYGLEAADVRQDGKLVHLHFRFEKGLEREFVYRVEEES

Nucleotide

• Length: 438 bp

>NTDB_id=1139653 DQM63_RS10535 WP_002901713.1 2114250..2114687(-) (comGF/cglF) [Streptococcus sanguinis strain NCTC7863]
ATGTCCAAAAACCTTAAAGTCAAAGCCTTTACTCTCTTGGAGACTTTAGTAGCCTTGCTGGTTCTCAGTGGCGGGGTGTT
AGTCTTTCAGGCTATGACCCAGCTCTTATCTTCAGAACTGCATCAGCAGGAGAACAATCAACAGCAAGAGTGGCTTTTGT
TTGCCGACCAACTGGAGACGGAGCTTTCGCGAAGTCAATTTGACAAGGTTGAAGACAATAAAATCTACATCAGACAGGAT
GGCAGGGACTTGGCCTTGGGTAAATCCAAGGGCGATGATTTCCGTAAAACAGATAAGAGCGGTCGCGGCTACCAGCCGAT
GATTTATGGTTTGGAAGCAGCTGATGTACGTCAGGACGGTAAGCTTGTCCATCTTCATTTTCGCTTTGAAAAAGGCTTAG
AGAGGGAGTTCGTCTATCGTGTGGAAGAAGAAAGTTGA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

3D structure

• AlphaFold DB: F2CFN3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF/cglF	Streptococcus pneumoniae Rx1	65.035	98.621	0.641
comGF/cglF	Streptococcus pneumoniae D39	65.035	98.621	0.641
comGF/cglF	Streptococcus pneumoniae R6	65.035	98.621	0.641
comGF/cglF	Streptococcus pneumoniae TIGR4	65.035	98.621	0.641
comGF/cglF	Streptococcus mitis SK321	63.636	98.621	0.628
comGF/cglF	Streptococcus mitis NCTC 12261	62.238	98.621	0.614
comYF	Streptococcus mutans UA159	47.857	96.552	0.462
comYF	Streptococcus mutans UA140	47.143	96.552	0.455
comGF	Lactococcus lactis subsp. cremoris KW2	41.912	93.793	0.393