Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   DQM66_RS09890 Genome accession   NZ_LS483374
Coordinates   1842548..1843489 (-) Length   313 a.a.
NCBI ID   WP_000249564.1    Uniprot ID   Q8DN86
Organism   Streptococcus pneumoniae strain NCTC7466     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1837548..1848489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM66_RS09850 (NCTC7466_01891) comYH 1838034..1838987 (-) 954 WP_000345135.1 class I SAM-dependent methyltransferase Machinery gene
  DQM66_RS09855 - 1839048..1839642 (-) 595 Protein_1837 class I SAM-dependent methyltransferase -
  DQM66_RS09860 (NCTC7466_01894) comGG/cglG 1839779..1840192 (-) 414 WP_000265622.1 competence type IV pilus minor pilin ComGG Machinery gene
  DQM66_RS09865 (NCTC7466_01895) comGF/cglF 1840170..1840631 (-) 462 WP_000250534.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQM66_RS09870 (NCTC7466_01896) comGE/cglE 1840594..1840896 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  DQM66_RS09875 (NCTC7466_01897) comGD/cglD 1840859..1841263 (-) 405 WP_000588026.1 competence type IV pilus minor pilin ComGD Machinery gene
  DQM66_RS09880 (NCTC7466_01898) comGC/cglC 1841256..1841582 (-) 327 WP_000738626.1 comG operon protein ComGC Machinery gene
  DQM66_RS09885 (NCTC7466_01899) comGB/cglB 1841584..1842600 (-) 1017 WP_074196785.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQM66_RS09890 (NCTC7466_01900) comGA/cglA/cilD 1842548..1843489 (-) 942 WP_000249564.1 competence type IV pilus ATPase ComGA Machinery gene
  DQM66_RS09895 (NCTC7466_01901) - 1843565..1843930 (-) 366 WP_000286412.1 DUF1033 family protein -
  DQM66_RS09900 (NCTC7466_01902) - 1844081..1845139 (-) 1059 WP_000649468.1 zinc-dependent alcohol dehydrogenase family protein -
  DQM66_RS09905 (NCTC7466_01903) nagA 1845302..1846453 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  DQM66_RS09910 (NCTC7466_01904) - 1846606..1848423 (-) 1818 WP_001220853.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35555.47 Da        Isoelectric Point: 6.1330

>NTDB_id=1139082 DQM66_RS09890 WP_000249564.1 1842548..1843489(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain NCTC7466]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDHKIASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFASRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1139082 DQM66_RS09890 WP_000249564.1 1842548..1843489(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain NCTC7466]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATCTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCATAAGATAGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACCTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGTCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAGCAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAGC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8DN86

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae D39

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae R6

100

100

1
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

100

100

1
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

95.847

100

0.958
  comYA Streptococcus gordonii str. Challis substr. CH1

78.065

99.042

0.773
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

41.912

86.901

0.364

Multiple sequence alignment