Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA   Type   Machinery gene
Locus tag   DQM52_RS00780 Genome accession   NZ_LS483367
Coordinates   128309..129250 (+) Length   313 a.a.
NCBI ID   WP_003062163.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain NCTC5371     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 123309..134250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM52_RS00770 (NCTC5371_00159) rpoC 124019..127660 (+) 3642 WP_037589802.1 DNA-directed RNA polymerase subunit beta' -
  DQM52_RS00775 (NCTC5371_00160) - 127863..128228 (+) 366 WP_003055257.1 DUF1033 family protein -
  DQM52_RS00780 (NCTC5371_00161) comGA/cglA 128309..129250 (+) 942 WP_003062163.1 competence type IV pilus ATPase ComGA Machinery gene
  DQM52_RS00785 (NCTC5371_00162) comYB 129186..130217 (+) 1032 WP_015057201.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQM52_RS00790 (NCTC5371_00163) comYC 130219..130545 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  DQM52_RS00795 (NCTC5371_00164) comGD 130520..130948 (+) 429 WP_014611932.1 competence type IV pilus minor pilin ComGD -
  DQM52_RS00800 (NCTC5371_00165) comGE 130956..131201 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  DQM52_RS00805 (NCTC5371_00166) comGF 131182..131622 (+) 441 WP_003061780.1 competence type IV pilus minor pilin ComGF -
  DQM52_RS00810 (NCTC5371_00167) comGG 131645..131974 (+) 330 WP_003058539.1 competence type IV pilus minor pilin ComGG -
  DQM52_RS00815 (NCTC5371_00168) comYH 132037..132990 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  DQM52_RS00820 (NCTC5371_00169) - 133049..134245 (+) 1197 WP_110407814.1 acetate kinase -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35166.27 Da        Isoelectric Point: 7.2836

>NTDB_id=1138815 DQM52_RS00780 WP_003062163.1 128309..129250(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC5371]
MVQALARSILKEAEQIQAQDIYILPKEDQYELLIRVGDERRLVDVYRGDRMAHLISHFKFVAGMTVGEKRRCQVGSCDYD
IGGDTLLSLRLSSVGNYRGQESLVIRLLHHQQRSLHYWFDGLKTVARQIGGRGLYLFAGPVGSGKTTLMHQLIADYYQGA
QVISIEDPVEIKNHQALQLQVNDSIGMTYDNLIKLSLRHRPDILIIGEIRDSQTARAVIRASLTGVLVFSTVHAKSISGV
YARLLELGVTRAELDNCLALVAYQRLLNGGALIDSTQTEFEHYSPNQWNQKIDDLLAAGHLSPEQARFEKIIQ

Nucleotide


Download         Length: 942 bp        

>NTDB_id=1138815 DQM52_RS00780 WP_003062163.1 128309..129250(+) (comGA/cglA) [Streptococcus dysgalactiae subsp. equisimilis strain NCTC5371]
ATGGTACAAGCATTAGCAAGATCTATTTTGAAAGAAGCTGAGCAGATTCAAGCTCAAGATATCTATATTTTGCCAAAGGA
AGATCAGTATGAGCTATTGATAAGGGTAGGAGATGAAAGGAGATTGGTGGATGTTTATCGGGGCGATCGGATGGCTCATC
TTATTAGTCACTTTAAGTTCGTTGCAGGAATGACCGTTGGTGAGAAACGACGCTGTCAAGTCGGTTCTTGTGATTATGAT
ATCGGTGGAGACACACTTTTGTCTCTACGCCTGTCTAGTGTTGGAAATTACCGTGGACAAGAGAGCCTAGTGATTCGGTT
ATTACATCATCAACAAAGAAGTTTACATTACTGGTTTGACGGACTGAAAACAGTTGCACGTCAGATTGGAGGCCGAGGAC
TCTATCTTTTTGCAGGTCCAGTCGGATCAGGTAAAACGACTTTGATGCACCAATTGATTGCTGATTATTATCAAGGAGCA
CAGGTCATTAGCATAGAAGATCCGGTGGAAATTAAAAATCATCAAGCGCTCCAATTACAAGTTAATGATAGTATTGGCAT
GACTTATGACAACTTGATCAAACTATCTTTACGGCATCGCCCTGATATTTTAATCATTGGTGAAATTCGAGACAGCCAGA
CAGCTAGAGCGGTTATTAGAGCAAGTTTAACAGGTGTCTTAGTATTTTCAACAGTTCATGCCAAAAGCATTTCTGGTGTT
TATGCCAGATTACTAGAACTAGGAGTGACAAGAGCAGAATTAGACAATTGTTTGGCTTTGGTTGCTTACCAACGATTGCT
TAATGGAGGAGCATTGATTGACTCAACTCAAACAGAATTTGAACACTACTCACCAAACCAATGGAATCAGAAAATTGATG
ACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

99.042

0.613
  comYA Streptococcus mutans UA140

61.415

99.361

0.61
  comYA Streptococcus mutans UA159

61.415

99.361

0.61
  comYA Streptococcus gordonii str. Challis substr. CH1

61.093

99.361

0.607
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

60.129

99.361

0.597
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

59.807

99.361

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

59.807

99.361

0.594
  comGA Lactococcus lactis subsp. cremoris KW2

52.09

99.361

0.518
  comGA Latilactobacillus sakei subsp. sakei 23K

38.908

93.61

0.364

Multiple sequence alignment