Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   DQM50_RS09450 Genome accession   NZ_LS483347
Coordinates   1830458..1831729 (-) Length   423 a.a.
NCBI ID   WP_030127534.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8324     
Function   require for competence (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1820737..1848944 1830458..1831729 within 0


Gene organization within MGE regions


Location: 1820737..1848944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM50_RS09400 (NCTC8324_01880) nrdG 1820737..1821351 (-) 615 WP_021775544.1 anaerobic ribonucleoside-triphosphate reductase activating protein -
  DQM50_RS09405 (NCTC8324_01881) - 1821366..1821860 (-) 495 WP_014407930.1 GNAT family N-acetyltransferase -
  DQM50_RS09410 (NCTC8324_01882) - 1821869..1822804 (-) 936 WP_076634441.1 Gfo/Idh/MocA family protein -
  DQM50_RS10025 (NCTC8324_01883) - 1822833..1822979 (-) 147 WP_023610752.1 hypothetical protein -
  DQM50_RS09415 (NCTC8324_01884) nrdD 1823161..1825359 (-) 2199 WP_085613596.1 anaerobic ribonucleoside-triphosphate reductase -
  DQM50_RS09420 (NCTC8324_01885) - 1825456..1827015 (-) 1560 WP_030126183.1 membrane protein -
  DQM50_RS09425 (NCTC8324_01887) - 1827429..1827734 (-) 306 WP_002982199.1 DUF1292 domain-containing protein -
  DQM50_RS09430 (NCTC8324_01888) ruvX 1827746..1828165 (-) 420 WP_085613595.1 Holliday junction resolvase RuvX -
  DQM50_RS09435 (NCTC8324_01889) - 1828162..1828431 (-) 270 WP_002982194.1 IreB family regulatory phosphoprotein -
  DQM50_RS09440 (NCTC8324_01890) spx 1828544..1828942 (-) 399 WP_002982188.1 transcriptional regulator Spx -
  DQM50_RS09445 (NCTC8324_01891) recA 1829233..1830369 (-) 1137 WP_002992179.1 recombinase RecA Machinery gene
  DQM50_RS09450 (NCTC8324_01892) cinA 1830458..1831729 (-) 1272 WP_030127534.1 competence/damage-inducible protein A Machinery gene
  DQM50_RS09455 (NCTC8324_01893) - 1831798..1832358 (-) 561 WP_085613594.1 DNA-3-methyladenine glycosylase I -
  DQM50_RS09460 (NCTC8324_01894) ruvA 1832368..1832961 (-) 594 WP_003058778.1 Holliday junction branch migration protein RuvA -
  DQM50_RS09465 (NCTC8324_01895) - 1832963..1834183 (-) 1221 WP_085613593.1 MDR family MFS transporter -
  DQM50_RS09470 (NCTC8324_01896) mutL 1834194..1836176 (-) 1983 WP_085613592.1 DNA mismatch repair endonuclease MutL -
  DQM50_RS09475 (NCTC8324_01897) - 1836271..1837422 (-) 1152 WP_023612650.1 tyrosine-type recombinase/integrase -
  DQM50_RS10250 - 1837744..1837887 (+) 144 WP_011185066.1 putative holin-like toxin -
  DQM50_RS09485 (NCTC8324_01899) - 1838758..1839438 (-) 681 WP_011185067.1 helix-turn-helix domain-containing protein -
  DQM50_RS09490 (NCTC8324_01900) - 1839602..1839799 (+) 198 WP_011185068.1 helix-turn-helix domain-containing protein -
  DQM50_RS09495 (NCTC8324_01901) - 1839813..1840433 (+) 621 WP_172451634.1 Bro-N domain-containing protein -
  DQM50_RS09500 (NCTC8324_01902) - 1840460..1840702 (+) 243 WP_000648623.1 hypothetical protein -
  DQM50_RS10030 (NCTC8324_01903) - 1840962..1841132 (+) 171 WP_021775540.1 hypothetical protein -
  DQM50_RS09505 (NCTC8324_01904) - 1841356..1841529 (+) 174 WP_000074663.1 plasmid mobilization protein -
  DQM50_RS09510 (NCTC8324_01905) - 1841715..1842119 (+) 405 WP_227868812.1 hypothetical protein -
  DQM50_RS09515 (NCTC8324_01906) - 1842221..1842553 (+) 333 WP_021775546.1 hypothetical protein -
  DQM50_RS09520 (NCTC8324_01907) - 1842550..1842771 (+) 222 WP_021775572.1 hypothetical protein -
  DQM50_RS09525 (NCTC8324_01908) - 1842774..1842965 (+) 192 WP_021775551.1 hypothetical protein -
  DQM50_RS09530 (NCTC8324_01909) - 1842977..1843306 (+) 330 WP_003058810.1 hypothetical protein -
  DQM50_RS09535 (NCTC8324_01910) - 1843309..1843581 (+) 273 WP_000051914.1 hypothetical protein -
  DQM50_RS09540 (NCTC8324_01911) - 1843582..1844448 (+) 867 WP_111688577.1 primase alpha helix C-terminal domain-containing protein -
  DQM50_RS09545 (NCTC8324_01912) - 1844460..1845854 (+) 1395 Protein_1786 VapE domain-containing protein -
  DQM50_RS09550 (NCTC8324_01914) - 1846148..1846402 (+) 255 WP_001069294.1 hypothetical protein -
  DQM50_RS09555 (NCTC8324_01915) - 1846399..1846572 (+) 174 WP_000694576.1 hypothetical protein -
  DQM50_RS10035 - 1846578..1846751 (+) 174 WP_168389368.1 hypothetical protein -
  DQM50_RS09560 (NCTC8324_01916) - 1846753..1847262 (+) 510 WP_021775557.1 hypothetical protein -
  DQM50_RS09565 (NCTC8324_01917) - 1847336..1847824 (+) 489 WP_002992480.1 hypothetical protein -
  DQM50_RS09570 (NCTC8324_01918) - 1848224..1848586 (+) 363 WP_020905578.1 DUF1492 domain-containing protein -
  DQM50_RS09575 (NCTC8324_01919) - 1848561..1848944 (+) 384 WP_111688579.1 ArpU family phage packaging/lysis transcriptional regulator -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45989.71 Da        Isoelectric Point: 4.9060

>NTDB_id=1137789 DQM50_RS09450 WP_030127534.1 1830458..1831729(-) (cinA) [Streptococcus pyogenes strain NCTC8324]
MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGDNEERLLSVITTASHRSDLVILCGGLGPTKDDLTKQT
LAKYLRKDLVYDEQACQKLDDFFAKRKPSSRTPNNERQAQVIEGSISLPNKTGLAVGGFITVDGISYVVLPGPPSELKPM
VNEELVPLLSKQYSTLYSKVLRFFGIGESQLVTVLSDFIENQTDPTIAPYAKTGEVTLRLSTKTENQALADKKLGQLEAQ
LLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKYDKTITAAESLTAGLFQSTLASFPGASQVFNGGFVTYSMEEKAKM
LGLPLEELKSHGVVSAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQNKVESIKVLISGRSRLDV
RYIATLHAFNMVRKTLLKLENLL

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=1137789 DQM50_RS09450 WP_030127534.1 1830458..1831729(-) (cinA) [Streptococcus pyogenes strain NCTC8324]
ATGAAAGCTGAACTGATTGCAGTAGGTACCGAAATTTTGACTGGTCAAATTGTGAATACCAATGCTCAATTTCTGTCGGA
AAAAATGGCAGAGCTAGGTATTGATGTCTATTTTCAAACGGCTGTTGGGGACAACGAGGAGCGTTTACTTTCAGTGATTA
CAACTGCTAGTCATCGTAGTGACTTGGTGATTTTATGTGGTGGCCTTGGTCCAACGAAAGATGATTTAACCAAACAAACT
TTAGCAAAGTACCTTAGGAAAGACTTGGTTTATGATGAGCAAGCTTGTCAGAAACTAGATGACTTTTTTGCTAAGCGCAA
GCCTTCATCACGGACACCAAATAATGAGCGACAGGCACAAGTGATTGAAGGGTCAATCTCTTTGCCAAATAAAACTGGTC
TTGCGGTTGGTGGGTTCATCACAGTCGATGGTATTAGTTATGTTGTCTTACCGGGTCCTCCAAGTGAATTGAAGCCGATG
GTAAATGAAGAATTGGTACCACTTCTATCAAAACAATACAGTACATTGTATTCAAAGGTACTACGCTTTTTTGGTATTGG
GGAAAGTCAGTTGGTAACGGTCTTGTCAGATTTTATTGAGAATCAAACTGATCCAACCATTGCTCCGTATGCTAAGACTG
GCGAAGTGACTCTTCGCTTATCAACAAAAACTGAAAACCAAGCTCTGGCAGATAAAAAGTTAGGTCAGCTAGAAGCGCAG
CTACTATCCCGAAAAACTCTTGAAGGTCAACCCTTAGCTGATGTCTTTTATGGCTATGGGGAGGATAATTCCTTAGCGCG
TGAGACATTTGAGCTCTTAGTAAAATATGATAAGACAATTACAGCAGCAGAAAGTCTAACCGCGGGATTATTTCAGTCAA
CTTTGGCGAGTTTTCCAGGAGCTTCTCAAGTATTCAATGGAGGCTTTGTGACTTATAGCATGGAAGAAAAAGCGAAAATG
CTAGGCCTTCCTTTAGAGGAGTTGAAATCGCATGGCGTTGTTAGTGCTTATACAGCCGAGGGGATGGCGGAGCAAGCAAG
GTTATTGACTGGTGCTGATATTGGGGTAAGTTTAACAGGTGTTGCCGGACCAGATATGTTGGAGGAACAGCCTGCAGGTA
CAGTTTTCATTGGCCTTGCCACTCAAAATAAGGTAGAATCAATAAAGGTTTTGATTAGCGGGCGAAGTCGTTTGGATGTG
CGCTATATCGCTACTTTACATGCCTTTAATATGGTCCGTAAAACTTTATTAAAACTTGAGAATTTGCTATAA

Domains

Predicted by InterproScan.

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)

Competence-damaged protein (CinA)

(263-416)

Damage-inducible protein CinA KH domain (CinA_KH)

(178-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mutans UA159

69.504

100

0.695
  cinA Streptococcus mitis SK321

69.544

98.582

0.686
  cinA Streptococcus mitis NCTC 12261

69.065

98.582

0.681
  cinA Streptococcus pneumoniae TIGR4

68.106

98.582

0.671
  cinA Streptococcus pneumoniae Rx1

68.106

98.582

0.671
  cinA Streptococcus pneumoniae R6

68.106

98.582

0.671
  cinA Streptococcus pneumoniae D39

67.866

98.582

0.669
  cinA Streptococcus suis isolate S10

53.125

98.345

0.522
  cinA Bacillus subtilis subsp. subtilis str. 168

46.651

98.818

0.461

Multiple sequence alignment