Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DQL69_RS00665 Genome accession   NZ_LS483345
Coordinates   101884..102822 (+) Length   312 a.a.
NCBI ID   WP_047235612.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC8231     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 96884..107822
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL69_RS00655 (NCTC8231_00132) rpoC 97651..101274 (+) 3624 WP_047235611.1 DNA-directed RNA polymerase subunit beta' -
  DQL69_RS00660 (NCTC8231_00133) - 101426..101791 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQL69_RS00665 (NCTC8231_00134) comYA 101884..102822 (+) 939 WP_047235612.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL69_RS00670 (NCTC8231_00135) comYB 102758..103792 (+) 1035 WP_231871599.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL69_RS00675 (NCTC8231_00136) comYC 103794..104120 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL69_RS00680 (NCTC8231_00137) comGD 104095..104523 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQL69_RS00685 (NCTC8231_00138) comGE 104480..104764 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQL69_RS00690 (NCTC8231_00139) comYF 104757..105191 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL69_RS00695 (NCTC8231_00140) comGG 105175..105501 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  DQL69_RS00700 (NCTC8231_00141) comYH 105599..106552 (+) 954 WP_002987790.1 class I SAM-dependent methyltransferase Machinery gene
  DQL69_RS00705 (NCTC8231_00142) - 106611..107807 (+) 1197 WP_011284424.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35087.35 Da        Isoelectric Point: 8.5799

>NTDB_id=1137625 DQL69_RS00665 WP_047235612.1 101884..102822(+) (comYA) [Streptococcus pyogenes strain NCTC8231]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISNYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YVRLLELGVTKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1137625 DQL69_RS00665 WP_047235612.1 101884..102822(+) (comYA) [Streptococcus pyogenes strain NCTC8231]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAATTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAGATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGTAAGATTGTTAGAACTTGGTGTAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.415

99.679

0.612
  comYA Streptococcus mutans UA140

61.415

99.679

0.612
  comYA Streptococcus gordonii str. Challis substr. CH1

60.772

99.679

0.606
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

39.31

92.949

0.365

Multiple sequence alignment