Detailed information NTG1137628

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQL69_RS00690
• Genome accession: NZ_LS483345
• Coordinates: 104757..105191 (+)
• Length: 144 a.a.
• NCBI ID: WP_002986542.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC8231
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 99757..110191

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL69_RS00660 (NCTC8231_00133)	-	101426..101791 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQL69_RS00665 (NCTC8231_00134)	comYA	101884..102822 (+)	939	WP_047235612.1	competence type IV pilus ATPase ComGA	Machinery gene
DQL69_RS00670 (NCTC8231_00135)	comYB	102758..103792 (+)	1035	WP_231871599.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQL69_RS00675 (NCTC8231_00136)	comYC	103794..104120 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
DQL69_RS00680 (NCTC8231_00137)	comGD	104095..104523 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
DQL69_RS00685 (NCTC8231_00138)	comGE	104480..104764 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
DQL69_RS00690 (NCTC8231_00139)	comYF	104757..105191 (+)	435	WP_002986542.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQL69_RS00695 (NCTC8231_00140)	comGG	105175..105501 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
DQL69_RS00700 (NCTC8231_00141)	comYH	105599..106552 (+)	954	WP_002987790.1	class I SAM-dependent methyltransferase	Machinery gene
DQL69_RS00705 (NCTC8231_00142)	-	106611..107807 (+)	1197	WP_011284424.1	acetate kinase	-
DQL69_RS00710	-	107994..108302 (+)	309	WP_011284425.1	hypothetical protein	-
DQL69_RS00715 (NCTC8231_00143)	proC	108385..109155 (-)	771	WP_032464773.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16573.20 Da
• Isoelectric Point: 10.3584

>NTDB_id=1137628 DQL69_RS00690 WP_002986542.1 104757..105191(+) (comYF) [Streptococcus pyogenes strain NCTC8231]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1137628 DQL69_RS00690 WP_002986542.1 104757..105191(+) (comYF) [Streptococcus pyogenes strain NCTC8231]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAGACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	45.985	95.139	0.437
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae D39	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae R6	43.609	92.361	0.403
comGF/cglF	Streptococcus pneumoniae TIGR4	43.609	92.361	0.403
comGF/cglF	Streptococcus mitis SK321	42.857	92.361	0.396