Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   DQL03_RS00780 Genome accession   NZ_LS483337
Coordinates   117426..118364 (+) Length   312 a.a.
NCBI ID   WP_063629337.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NCTC12047     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 112426..123364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQL03_RS00770 (NCTC12047_00153) rpoC 113175..116816 (+) 3642 WP_011284419.1 DNA-directed RNA polymerase subunit beta' -
  DQL03_RS00775 (NCTC12047_00154) - 116968..117333 (+) 366 WP_002986560.1 DUF1033 family protein -
  DQL03_RS00780 (NCTC12047_00155) comYA 117426..118364 (+) 939 WP_063629337.1 competence type IV pilus ATPase ComGA Machinery gene
  DQL03_RS00785 (NCTC12047_00156) comYB 118300..119334 (+) 1035 WP_227874845.1 competence type IV pilus assembly protein ComGB Machinery gene
  DQL03_RS00790 (NCTC12047_00157) comYC 119336..119662 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  DQL03_RS00795 (NCTC12047_00158) comGD 119637..120065 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  DQL03_RS00800 (NCTC12047_00159) comGE 120022..120306 (+) 285 WP_002987779.1 competence type IV pilus minor pilin ComGE -
  DQL03_RS00805 (NCTC12047_00160) comYF 120299..120733 (+) 435 WP_010921800.1 competence type IV pilus minor pilin ComGF Machinery gene
  DQL03_RS00810 (NCTC12047_00161) comGG 120717..121043 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  DQL03_RS00815 (NCTC12047_00162) comYH 121141..122094 (+) 954 WP_010921802.1 class I SAM-dependent methyltransferase Machinery gene
  DQL03_RS00820 (NCTC12047_00163) - 122153..123349 (+) 1197 WP_010921803.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35028.27 Da        Isoelectric Point: 8.5799

>NTDB_id=1137225 DQL03_RS00780 WP_063629337.1 117426..118364(+) (comYA) [Streptococcus pyogenes strain NCTC12047]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLLYQLISSYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGITKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1137225 DQL03_RS00780 WP_063629337.1 117426..118364(+) (comYA) [Streptococcus pyogenes strain NCTC12047]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGTTGTACCAGCTGATTTCGAGTTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAAATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTATAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.415

99.679

0.612
  comYA Streptococcus mutans UA140

61.415

99.679

0.612
  comYA Streptococcus gordonii str. Challis substr. CH1

60.129

99.679

0.599
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60

99.359

0.596
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.164

99.679

0.59
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.521

99.679

0.583
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.521

99.679

0.583
  comGA Lactococcus lactis subsp. cremoris KW2

50.161

99.679

0.5
  comGA Latilactobacillus sakei subsp. sakei 23K

40.345

92.949

0.375

Multiple sequence alignment