Detailed information NTG1137228

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: DQL03_RS00805
• Genome accession: NZ_LS483337
• Coordinates: 120299..120733 (+)
• Length: 144 a.a.
• NCBI ID: WP_010921800.1
• Uniprot ID: -
• Organism: Streptococcus pyogenes strain NCTC12047
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 115299..125733

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DQL03_RS00775 (NCTC12047_00154)	-	116968..117333 (+)	366	WP_002986560.1	DUF1033 family protein	-
DQL03_RS00780 (NCTC12047_00155)	comYA	117426..118364 (+)	939	WP_063629337.1	competence type IV pilus ATPase ComGA	Machinery gene
DQL03_RS00785 (NCTC12047_00156)	comYB	118300..119334 (+)	1035	WP_227874845.1	competence type IV pilus assembly protein ComGB	Machinery gene
DQL03_RS00790 (NCTC12047_00157)	comYC	119336..119662 (+)	327	WP_002986552.1	competence type IV pilus major pilin ComGC	Machinery gene
DQL03_RS00795 (NCTC12047_00158)	comGD	119637..120065 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
DQL03_RS00800 (NCTC12047_00159)	comGE	120022..120306 (+)	285	WP_002987779.1	competence type IV pilus minor pilin ComGE	-
DQL03_RS00805 (NCTC12047_00160)	comYF	120299..120733 (+)	435	WP_010921800.1	competence type IV pilus minor pilin ComGF	Machinery gene
DQL03_RS00810 (NCTC12047_00161)	comGG	120717..121043 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
DQL03_RS00815 (NCTC12047_00162)	comYH	121141..122094 (+)	954	WP_010921802.1	class I SAM-dependent methyltransferase	Machinery gene
DQL03_RS00820 (NCTC12047_00163)	-	122153..123349 (+)	1197	WP_010921803.1	acetate kinase	-
DQL03_RS00825	-	123536..123844 (+)	309	Protein_114	hypothetical protein	-
DQL03_RS00830 (NCTC12047_00164)	proC	123927..124697 (-)	771	WP_002986527.1	pyrroline-5-carboxylate reductase	-

Sequence

Protein

• Length: 144 a.a.
• Molecular weight: 16654.28 Da
• Isoelectric Point: 10.2526

>NTDB_id=1137228 DQL03_RS00805 WP_010921800.1 120299..120733(+) (comYF) [Streptococcus pyogenes strain NCTC12047]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTRTLLKHSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIEQSQICITLKWKSGLERTFYYAFQD

Nucleotide

• Length: 435 bp

>NTDB_id=1137228 DQL03_RS00805 WP_010921800.1 120299..120733(+) (comYF) [Streptococcus pyogenes strain NCTC12047]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCGTTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCGAACCCTCCTTAAACATAGCCATTATCTAGCCCGTCATGATCAAGATAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAA
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTTCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGAGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-135)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	51.079	96.528	0.493
comYF	Streptococcus mutans UA159	50.36	96.528	0.486
comGF	Lactococcus lactis subsp. cremoris KW2	47.445	95.139	0.451
comGF/cglF	Streptococcus mitis NCTC 12261	44.03	93.056	0.41
comGF/cglF	Streptococcus pneumoniae Rx1	42.857	92.361	0.396
comGF/cglF	Streptococcus pneumoniae D39	42.857	92.361	0.396
comGF/cglF	Streptococcus pneumoniae R6	42.857	92.361	0.396
comGF/cglF	Streptococcus pneumoniae TIGR4	42.857	92.361	0.396
comGF/cglF	Streptococcus mitis SK321	42.105	92.361	0.389