Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DENOEST_RS01415 Genome accession   NZ_LR778301
Coordinates   265470..266687 (+) Length   405 a.a.
NCBI ID   WP_145771420.1    Uniprot ID   A0A6S6XNG2
Organism   Denitratisoma oestradiolicum strain DSM 16959     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 260470..271687
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DENOEST_RS01400 (DENOEST_0296) - 262087..262707 (+) 621 WP_145771417.1 lytic transglycosylase domain-containing protein -
  DENOEST_RS01405 (DENOEST_0297) ffh 262967..264322 (-) 1356 WP_145771418.1 signal recognition particle protein -
  DENOEST_RS01410 (DENOEST_0298) - 264365..265195 (+) 831 WP_145771419.1 inner membrane protein YpjD -
  DENOEST_RS01415 (DENOEST_0299) pilC 265470..266687 (+) 1218 WP_145771420.1 type II secretion system F family protein Machinery gene
  DENOEST_RS01420 (DENOEST_0300) pilD 266687..267541 (+) 855 WP_145771421.1 A24 family peptidase Machinery gene
  DENOEST_RS01425 (DENOEST_0301) - 267637..267900 (+) 264 WP_145771422.1 FmdB family zinc ribbon protein -
  DENOEST_RS01430 (DENOEST_0302) - 267902..268522 (+) 621 WP_145771423.1 DUF502 domain-containing protein -
  DENOEST_RS01435 (DENOEST_0303) aspS 268575..270371 (+) 1797 WP_145771424.1 aspartate--tRNA ligase -
  DENOEST_RS01440 (DENOEST_0304) - 270449..270775 (+) 327 WP_232096410.1 nucleotidyltransferase domain-containing protein -
  DENOEST_RS01445 (DENOEST_0305) - 270759..271154 (+) 396 WP_197970479.1 HEPN domain-containing protein -
  DENOEST_RS01450 (DENOEST_0306) - 271151..271534 (+) 384 WP_332068209.1 ribonuclease domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43723.59 Da        Isoelectric Point: 9.7067

>NTDB_id=1130254 DENOEST_RS01415 WP_145771420.1 265470..266687(+) (pilC) [Denitratisoma oestradiolicum strain DSM 16959]
MATAKDDKKKEISFVWEGKDKAGRIMRGEMRAGGEAVVQATLRRQGILVSKIKKQASGGGGKVTAKDITLFTRQLATMMK
AGVPLLQSFDIVGKGSSNPAVAKLLFDIKTEVETGSSLNQAFRKYPMYFDLLFCNLVEAGEQAGILEMLLDRLASYKEKI
LAIQSKIKSALFYPIAVVMVAFVVTAILMIFVVPQFKSVFSGFGADLPAPTLVVVGISDFFVKYWYVIFGGLGGGVYGFL
NLWKRSRKVQIFMDRLSLKLPVFGDLLTKATIARWARTLSTMFAAGVPLVEALDSVGGAAGNYVYATATKQIQAEVSTGN
SLTVAMQNANVFPSMVLQMVAIGEESGQLDSMLSKVADFFEAEVDDAVEALSSLMEPIIMAFLGVVVGGMVVAMYLPIFK
LGAVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1130254 DENOEST_RS01415 WP_145771420.1 265470..266687(+) (pilC) [Denitratisoma oestradiolicum strain DSM 16959]
ATGGCTACGGCGAAGGACGATAAAAAGAAGGAAATCAGTTTCGTCTGGGAAGGCAAGGACAAGGCTGGCCGCATCATGCG
CGGCGAAATGCGCGCGGGGGGCGAGGCGGTGGTTCAGGCCACCCTGCGGCGCCAGGGCATCCTGGTCAGCAAGATCAAAA
AGCAGGCCAGTGGCGGCGGCGGCAAGGTCACCGCCAAGGACATCACTCTGTTTACCCGGCAACTGGCCACCATGATGAAG
GCTGGCGTGCCCCTGCTCCAGTCCTTCGATATTGTTGGCAAGGGTTCCTCCAACCCAGCAGTGGCCAAGCTTTTGTTCGA
CATCAAGACCGAGGTGGAAACCGGTTCCAGTCTGAATCAGGCCTTTCGCAAGTACCCCATGTACTTCGACCTGCTGTTCT
GCAACCTGGTGGAGGCCGGCGAGCAGGCGGGCATCCTGGAAATGCTGCTGGACCGGCTGGCCAGCTACAAGGAAAAGATC
CTGGCCATCCAGTCCAAGATCAAGAGCGCCCTGTTCTATCCCATCGCGGTGGTCATGGTGGCCTTTGTGGTGACCGCGAT
CCTGATGATCTTCGTGGTACCCCAGTTCAAGTCCGTGTTCTCCGGCTTCGGTGCCGACCTGCCGGCACCAACCCTGGTAG
TGGTGGGAATTTCCGACTTTTTCGTCAAGTATTGGTATGTCATCTTTGGTGGTCTCGGTGGGGGTGTTTACGGTTTTCTC
AACCTCTGGAAGCGATCCCGGAAGGTGCAGATATTCATGGATCGACTATCCCTCAAGTTGCCGGTCTTCGGCGATCTGCT
GACGAAAGCCACCATTGCCCGCTGGGCACGTACCCTGTCCACCATGTTTGCTGCCGGCGTACCCCTGGTCGAGGCTCTGG
ACTCCGTGGGCGGCGCGGCGGGCAACTATGTCTACGCCACGGCCACCAAACAGATCCAGGCCGAGGTCTCCACCGGCAAC
AGCCTCACGGTGGCCATGCAGAACGCCAATGTCTTCCCCTCCATGGTCCTCCAGATGGTGGCCATTGGCGAGGAGTCCGG
TCAACTGGATAGCATGCTGAGCAAGGTGGCGGACTTTTTCGAGGCGGAAGTGGATGACGCGGTCGAGGCCCTGTCCAGCC
TGATGGAGCCCATCATCATGGCTTTCCTGGGCGTGGTGGTGGGGGGGATGGTGGTGGCCATGTATCTGCCGATCTTCAAG
CTTGGCGCCGTGGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S6XNG2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

56.391

98.519

0.556
  pilC Legionella pneumophila strain ERS1305867

56.281

98.272

0.553
  pilC Acinetobacter baumannii D1279779

53.769

98.272

0.528
  pilC Acinetobacter baylyi ADP1

52.645

98.025

0.516
  pilG Neisseria gonorrhoeae MS11

50.754

98.272

0.499
  pilG Neisseria meningitidis 44/76-A

50.503

98.272

0.496
  pilC Vibrio cholerae strain A1552

41.31

98.025

0.405
  pilC Vibrio campbellii strain DS40M4

39.109

99.753

0.39
  pilC Thermus thermophilus HB27

37.923

100

0.388

Multiple sequence alignment