Detailed information NTG1128184

Bioinformatically predicted

Overview

• Name: pilC
• Type: Machinery gene
• Locus tag: E5P3_RS08025
• Genome accession: NZ_LR594662
• Coordinates: 1678234..1679457 (-)
• Length: 407 a.a.
• NCBI ID: WP_162585492.1
• Uniprot ID: A0A6P2E678
• Organism: Variovorax sp. RA8
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1673234..1684457

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
E5P3_RS08000 (G3W91_RS08005)	zapD	1673275..1674030 (-)	756	WP_068679953.1	cell division protein ZapD	-
E5P3_RS08005 (G3W91_RS08010)	-	1674068..1675084 (-)	1017	WP_162585488.1	HAD family hydrolase	-
E5P3_RS08010 (G3W91_RS08015)	-	1675095..1676588 (-)	1494	WP_162585489.1	MBOAT family protein	-
E5P3_RS08015 (G3W91_RS08020)	coaE	1676673..1677311 (-)	639	WP_162585490.1	dephospho-CoA kinase	-
E5P3_RS08020 (G3W91_RS08025)	-	1677269..1678234 (-)	966	WP_162585491.1	A24 family peptidase	-
E5P3_RS08025 (G3W91_RS08030)	pilC	1678234..1679457 (-)	1224	WP_162585492.1	type II secretion system F family protein	Machinery gene
E5P3_RS08030 (G3W91_RS08035)	pilB	1679473..1681206 (-)	1734	WP_162585493.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 407 a.a.
• Molecular weight: 44566.31 Da
• Isoelectric Point: 9.5100

>NTDB_id=1128184 E5P3_RS08025 WP_162585492.1 1678234..1679457(-) (pilC) [Variovorax sp. RA8]
MATAAASRTTLKEYVFEWEGKDRNGKVVRGEIRAAGENQVQAALRRQGVLASKIKKRRMRAGKAIKPKDIAIFTRQLATM
MKAGVPLLQAFDIVGRGNANPSVAKLLNDIRSDVETGTSLSAAFRKFPKYFDNLYCNLVEAGEAAGILEELLDRLATYME
KTEAIKSKIKSALMYPTSVVVVAFVVVAIIMIFVIPAFKQVFTSFGADLPAPTLFVMGMSEFFVDYWWLIFGVIGGGLYF
FFQAWKRNERVQRFMDRLLLRVPIFGALIEKSCVARWTRTLSTMFAAGVPLVEALDSVGGASGNTVYGDATVKIQQEVST
GTSLTMAMTNASLFPSMVLQMTAIGEESGSIDHMLGKAADFYESEVDDMVAGLSSLMEPIIIVFLGVIIGGIVVSMYLPI
FKLGQVV

Nucleotide

• Length: 1224 bp

>NTDB_id=1128184 E5P3_RS08025 WP_162585492.1 1678234..1679457(-) (pilC) [Variovorax sp. RA8]
ATGGCAACAGCAGCAGCATCCCGGACGACGCTCAAGGAATACGTCTTCGAGTGGGAAGGCAAGGACCGCAACGGCAAGGT
GGTGCGCGGCGAGATCCGCGCCGCAGGCGAGAACCAGGTCCAGGCCGCATTGCGCCGCCAGGGCGTGCTCGCCTCGAAGA
TCAAGAAGCGGCGCATGCGCGCCGGCAAGGCCATCAAGCCCAAGGACATCGCCATCTTCACGCGCCAGCTGGCGACCATG
ATGAAGGCCGGCGTCCCGCTGCTGCAGGCGTTCGACATCGTGGGCCGCGGCAATGCCAACCCGAGTGTGGCCAAGCTGCT
CAACGACATCCGCAGCGATGTCGAGACCGGCACCTCGCTGTCCGCCGCCTTCCGCAAGTTCCCGAAGTACTTCGACAACC
TCTACTGCAACCTGGTCGAGGCCGGCGAGGCCGCCGGTATCCTGGAAGAACTGCTCGACCGCCTGGCCACCTACATGGAG
AAGACGGAGGCCATCAAGTCGAAGATCAAGTCGGCGCTGATGTACCCCACCTCGGTGGTCGTGGTGGCCTTCGTGGTGGT
GGCGATCATCATGATCTTCGTGATCCCGGCCTTCAAGCAGGTGTTCACCTCCTTCGGCGCCGACCTGCCGGCGCCGACGC
TCTTCGTGATGGGCATGAGCGAGTTCTTCGTCGATTACTGGTGGCTGATCTTCGGCGTGATCGGCGGCGGGCTGTACTTC
TTCTTCCAGGCCTGGAAGCGCAACGAGCGCGTCCAGCGCTTCATGGACCGCCTGCTGCTGCGCGTGCCGATCTTCGGTGC
GCTGATCGAGAAATCCTGCGTGGCCCGCTGGACCCGCACCCTGTCCACCATGTTCGCGGCGGGCGTGCCGCTCGTGGAAG
CCCTCGATTCCGTGGGCGGCGCCTCGGGCAACACGGTGTACGGCGACGCCACGGTCAAGATCCAGCAGGAAGTCTCGACG
GGCACCAGCTTGACGATGGCCATGACCAACGCCAGCCTGTTTCCCTCGATGGTGCTGCAGATGACGGCCATCGGCGAGGA
GTCCGGCTCCATCGACCACATGCTGGGCAAGGCTGCCGATTTCTACGAGTCGGAGGTCGATGACATGGTCGCGGGCCTGT
CGAGCCTGATGGAGCCGATCATCATCGTGTTCCTCGGGGTGATCATCGGCGGCATCGTGGTTTCGATGTACCTGCCCATC
TTCAAGCTCGGCCAGGTCGTCTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (277-399)

• Type II secretion system (T2SS), protein F (T2SSF) (73-196)

3D structure

• AlphaFold DB: A0A6P2E678

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilC	Pseudomonas stutzeri DSM 10701	53.96	99.263	0.536
pilC	Acinetobacter baylyi ADP1	50.86	100	0.509
pilG	Neisseria gonorrhoeae MS11	52.141	97.543	0.509
pilG	Neisseria meningitidis 44/76-A	51.889	97.543	0.506
pilC	Legionella pneumophila strain ERS1305867	50.505	97.297	0.491
pilC	Acinetobacter baumannii D1279779	48.866	97.543	0.477
pilC	Vibrio cholerae strain A1552	41.31	97.543	0.403
pilC	Vibrio campbellii strain DS40M4	39.803	100	0.398
pilC	Thermus thermophilus HB27	39.558	100	0.396