Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   E5P3_RS08030 Genome accession   NZ_LR594662
Coordinates   1679473..1681206 (-) Length   577 a.a.
NCBI ID   WP_162585493.1    Uniprot ID   -
Organism   Variovorax sp. RA8     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1674473..1686206
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  E5P3_RS08010 (G3W91_RS08015) - 1675095..1676588 (-) 1494 WP_162585489.1 MBOAT family protein -
  E5P3_RS08015 (G3W91_RS08020) coaE 1676673..1677311 (-) 639 WP_162585490.1 dephospho-CoA kinase -
  E5P3_RS08020 (G3W91_RS08025) - 1677269..1678234 (-) 966 WP_162585491.1 A24 family peptidase -
  E5P3_RS08025 (G3W91_RS08030) pilC 1678234..1679457 (-) 1224 WP_162585492.1 type II secretion system F family protein Machinery gene
  E5P3_RS08030 (G3W91_RS08035) pilB 1679473..1681206 (-) 1734 WP_162585493.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62497.74 Da        Isoelectric Point: 5.6405

>NTDB_id=1128185 E5P3_RS08030 WP_162585493.1 1679473..1681206(-) (pilB) [Variovorax sp. RA8]
MAAAEPLSKESASIALPGLARALISAGKLPAKTAEEIYQKSLSGRSSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
VDSQRLPKDLLDSKLCQAYRVVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVVAEYDKLSRMIEAAAVSAAETI
DSIVGAEFEFDDSSIAESTDANEAAIAEVEDAPVVRFLHKMLLDAFSMRASDIHFEPYEHQYRVRFRIDGELREIATPPT
VIKDKLASRIKVISRLDISEKRVPQDGRMKLKIGPDRVIDFRVSTLPTLFGEKIVVRILDPSSARLGIDALGYDADEKER
LMQAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNDKAGLTFAAALRAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCKAPTEVPR
EALLEAGFKDRDIDGTWKPYKPVGCPSCNGGYKGRVGIYQVMPISEEIQKIILRDGSALEIARQSEAEGVRSLRSSGLRK
VMQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1128185 E5P3_RS08030 WP_162585493.1 1679473..1681206(-) (pilB) [Variovorax sp. RA8]
ATGGCCGCTGCTGAACCTCTCTCCAAAGAAAGCGCATCGATCGCCCTGCCCGGGCTGGCGCGCGCCTTGATCTCCGCCGG
CAAGTTGCCGGCGAAGACGGCGGAGGAGATCTATCAGAAGTCCCTTAGCGGGCGAAGCAGCTTCATTGCCGAGCTGACCG
GCACCGGCGCGGTCTCTGCGGCGGATCTCGCCCACACCCTGTCGAGCGCCTTCGGCGCCCCCCTGCTCGACCTCGACGCC
GTCGATTCCCAGCGCCTGCCCAAGGACCTGCTTGACTCCAAGCTGTGCCAGGCGTACCGGGTCGTGGTGCTCAGCAAGCG
CAACAATCGTCTCATCGTCGCAACAGCCGACCCCTCGGACCAGCAGGCGGCGGAAAAAATAAAGTTCGCGTCGCAGATGG
GCGTGGATTGGGTCGTTGCCGAGTACGACAAGCTCTCGCGCATGATCGAGGCGGCGGCCGTGAGCGCCGCCGAAACCATC
GACAGCATCGTCGGCGCCGAGTTCGAATTCGACGACTCCTCGATCGCCGAGAGCACCGACGCGAACGAAGCGGCGATCGC
CGAGGTGGAGGATGCACCGGTCGTCCGCTTCCTGCACAAGATGCTGCTCGACGCCTTCAGCATGCGTGCGTCGGACATCC
ACTTCGAGCCCTACGAGCACCAGTACCGCGTGCGCTTCCGGATCGACGGCGAGCTGCGCGAGATCGCGACCCCGCCCACC
GTCATCAAGGACAAGCTGGCCTCGCGCATCAAGGTGATCTCGCGGCTGGACATCTCCGAGAAGCGCGTGCCGCAGGACGG
CCGCATGAAGCTCAAGATCGGCCCGGACCGGGTGATCGACTTCCGCGTCAGCACCTTGCCCACGCTGTTCGGCGAGAAAA
TCGTGGTGCGTATCCTCGACCCGAGCAGCGCGCGCCTGGGCATCGACGCCCTGGGTTACGACGCCGACGAGAAAGAGCGG
CTGATGCAGGCCATCGGCCGCCCCTACGGCATGGTGCTGGTCACCGGGCCGACGGGCTCGGGCAAGACGGTCTCGCTCTA
CACCTGCCTGAACCTGCTGAACAAGCCGGGCGTCAACATCGCCACGGCCGAGGACCCGTCCGAAATCAACCTGCCCGGGG
TCAACCAGGTCAACGTCAACGACAAGGCGGGGCTGACCTTCGCAGCCGCGCTGCGCGCCTTCCTGCGGCAGGACCCGGAC
ATCATCATGGTCGGCGAAATACGCGACCTGGAAACCGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCT
GTCGACCCTCCACACCAACGATGCCCCGACCACGCTGACACGGATGCGCAACATGGGCATCGCGCCCTTCAATATCGCCT
CCAGCGTGATCCTGATCACCGCGCAGCGGCTCGCGCGCCGCCTGTGCACCGTGTGCAAGGCGCCGACCGAGGTGCCCCGG
GAAGCACTGCTCGAAGCCGGCTTCAAGGACCGCGACATCGACGGCACGTGGAAGCCTTACAAGCCCGTCGGCTGCCCGTC
TTGCAACGGCGGCTACAAGGGCCGCGTCGGCATCTACCAGGTGATGCCGATCTCCGAGGAAATCCAGAAGATCATCCTGC
GCGACGGAAGCGCGCTCGAGATCGCCCGGCAGTCCGAGGCCGAGGGTGTGCGCTCCCTGCGCAGTTCCGGGCTGCGCAAG
GTCATGCAGGGCCTGACTTCCCTCGAAGAAGTGCTCGCGGTCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.258

97.227

0.537
  pilB Acinetobacter baumannii D1279779

53.846

99.133

0.534
  pilF Neisseria gonorrhoeae MS11

53.464

97.574

0.522
  pilB Legionella pneumophila strain ERS1305867

49.385

98.614

0.487
  pilB Vibrio cholerae strain A1552

46.786

97.054

0.454
  pilB Vibrio campbellii strain DS40M4

45.826

97.574

0.447
  pilB Vibrio parahaemolyticus RIMD 2210633

47.834

92.028

0.44
  pilF Thermus thermophilus HB27

40.102

100

0.411
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.651

94.454

0.393

Multiple sequence alignment