Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssbA   Type   Machinery gene
Locus tag   FGL04_RS00710 Genome accession   NZ_LR594035
Coordinates   111248..111643 (+) Length   131 a.a.
NCBI ID   WP_138067858.1    Uniprot ID   A0A4U9XJN4
Organism   Streptococcus pseudoporcinus strain NCTC5385     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 106076..119337 111248..111643 within 0
IScluster/Tn 111886..113019 111248..111643 flank 243


Gene organization within MGE regions


Location: 106076..119337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGL04_RS00670 (NCTC5385_00135) - 106076..106285 (+) 210 WP_007895522.1 helix-turn-helix transcriptional regulator -
  FGL04_RS00675 (NCTC5385_00136) - 106282..106734 (+) 453 WP_138067852.1 hypothetical protein -
  FGL04_RS00680 (NCTC5385_00137) - 106746..107393 (+) 648 WP_138067853.1 CPBP family intramembrane glutamic endopeptidase -
  FGL04_RS00685 (NCTC5385_00138) proC 107571..108341 (-) 771 WP_138067854.1 pyrroline-5-carboxylate reductase -
  FGL04_RS00690 (NCTC5385_00139) pepA 108360..109427 (-) 1068 WP_138067855.1 glutamyl aminopeptidase -
  FGL04_RS00695 (NCTC5385_00140) - 109625..109912 (+) 288 WP_138067856.1 DUF4651 domain-containing protein -
  FGL04_RS00700 (NCTC5385_00141) - 109905..110240 (+) 336 WP_007895267.1 thioredoxin family protein -
  FGL04_RS00705 (NCTC5385_00142) ytpR 110240..110866 (+) 627 WP_138067857.1 YtpR family tRNA-binding protein -
  FGL04_RS10650 (NCTC5385_00143) - 110910..111080 (-) 171 WP_171011214.1 hypothetical protein -
  FGL04_RS00710 (NCTC5385_00144) ssbA 111248..111643 (+) 396 WP_138067858.1 single-stranded DNA-binding protein Machinery gene
  FGL04_RS00715 (NCTC5385_00146) - 111886..113019 (+) 1134 Protein_108 IS3 family transposase -
  FGL04_RS00720 (NCTC5385_00147) - 113077..113478 (-) 402 WP_138067859.1 hypothetical protein -
  FGL04_RS00725 (NCTC5385_00148) - 113800..115167 (+) 1368 WP_225247739.1 M protein trans-acting positive regulator PRD domain-containing protein -
  FGL04_RS00730 (NCTC5385_00149) hslO 115433..116305 (+) 873 WP_138067860.1 Hsp33 family molecular chaperone HslO -
  FGL04_RS00735 (NCTC5385_00150) dusB 116292..117269 (+) 978 WP_138067861.1 tRNA dihydrouridine synthase DusB -
  FGL04_RS00740 (NCTC5385_00151) - 117288..117929 (+) 642 WP_138067862.1 deoxynucleoside kinase -

Sequence


Protein


Download         Length: 131 a.a.        Molecular weight: 14999.18 Da        Isoelectric Point: 5.2863

>NTDB_id=1127278 FGL04_RS00710 WP_138067858.1 111248..111643(+) (ssbA) [Streptococcus pseudoporcinus strain NCTC5385]
MYNKVIMIGRLVSDPDLIKTPNDKHMCRVSIAVNRRFKNAEGERQVDFVNLVLWGRLAESLASYGSKGSLVSLDGELRIR
KYEKDDKTAYMTEVLCQSYQLLESRAQRAVRENNLADDLCDLLLDEEALPF

Nucleotide


Download         Length: 396 bp        

>NTDB_id=1127278 FGL04_RS00710 WP_138067858.1 111248..111643(+) (ssbA) [Streptococcus pseudoporcinus strain NCTC5385]
ATGTATAATAAAGTAATTATGATTGGTCGACTGGTTAGTGATCCTGATTTAATTAAGACGCCAAACGATAAGCACATGTG
CCGTGTTTCAATTGCGGTCAATCGACGGTTCAAAAATGCCGAAGGTGAACGTCAAGTTGATTTTGTTAATCTTGTTCTTT
GGGGCCGATTGGCTGAAAGTCTGGCTTCGTATGGTAGCAAGGGTAGTCTGGTTTCGCTTGATGGAGAGCTACGTATCCGC
AAGTACGAAAAAGACGACAAGACAGCCTATATGACTGAGGTTTTATGCCAATCCTATCAACTCTTAGAGAGTCGCGCTCA
GCGGGCTGTTAGAGAAAATAACTTGGCTGATGATCTTTGTGATTTACTTCTAGACGAAGAGGCACTCCCTTTTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A4U9XJN4

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssbA Streptococcus mutans UA159

70.992

100

0.71

  ssbB Streptococcus sobrinus strain NIDR 6715-7

67.176

100

0.672

  ssbB/cilA Streptococcus pneumoniae Rx1

67.176

100

0.672

  ssbB/cilA Streptococcus pneumoniae D39

67.176

100

0.672

  ssbB/cilA Streptococcus pneumoniae R6

67.176

100

0.672

  ssbB/cilA Streptococcus mitis NCTC 12261

67.176

100

0.672

  ssbB/cilA Streptococcus pneumoniae TIGR4

66.412

100

0.664

  ssbB/cilA Streptococcus mitis SK321

65.649

100

0.656

  ssbB Lactococcus lactis subsp. cremoris KW2

53.571

85.496

0.458

  ssb Latilactobacillus sakei subsp. sakei 23K

42.735

89.313

0.382


Multiple sequence alignment