Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PFLUOLIPICF7_RS04290 Genome accession   NZ_CP005975
Coordinates   938933..940141 (+) Length   402 a.a.
NCBI ID   WP_010213233.1    Uniprot ID   A0A1N7UA21
Organism   Pseudomonas simiae strain PICF7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 933933..945141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFLUOLIPICF7_RS04265 (PFLUOLIPICF7_04310) - 935547..936261 (+) 715 Protein_857 nicotinate-nucleotide diphosphorylase -
  PFLUOLIPICF7_RS04270 (PFLUOLIPICF7_04315) - 936309..936614 (-) 306 WP_010213230.1 DUF6388 family protein -
  PFLUOLIPICF7_RS04280 (PFLUOLIPICF7_04325) - 936881..938281 (-) 1401 WP_042571063.1 O-antigen ligase family protein -
  PFLUOLIPICF7_RS04285 (PFLUOLIPICF7_04330) - 938317..938739 (-) 423 WP_021491914.1 pilin -
  PFLUOLIPICF7_RS04290 (PFLUOLIPICF7_04335) pilC 938933..940141 (+) 1209 WP_010213233.1 type II secretion system F family protein Machinery gene
  PFLUOLIPICF7_RS04295 (PFLUOLIPICF7_04340) pilD 940138..941004 (+) 867 WP_010213234.1 prepilin peptidase Machinery gene
  PFLUOLIPICF7_RS04300 (PFLUOLIPICF7_04345) coaE 941001..941624 (+) 624 WP_010213235.1 dephospho-CoA kinase -
  PFLUOLIPICF7_RS04305 (PFLUOLIPICF7_04350) yacG 941621..941821 (+) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  PFLUOLIPICF7_RS04310 (PFLUOLIPICF7_04355) - 941818..942033 (-) 216 WP_003171680.1 hypothetical protein -
  PFLUOLIPICF7_RS04315 (PFLUOLIPICF7_04360) - 942109..942798 (-) 690 WP_042571059.1 energy-coupling factor ABC transporter permease -
  PFLUOLIPICF7_RS04320 (PFLUOLIPICF7_04365) - 942795..943262 (-) 468 WP_042571058.1 hypothetical protein -
  PFLUOLIPICF7_RS04325 (PFLUOLIPICF7_04370) - 943366..943995 (+) 630 WP_010213242.1 DUF1780 domain-containing protein -
  PFLUOLIPICF7_RS04330 (PFLUOLIPICF7_04375) - 944050..944223 (+) 174 WP_010213243.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43341.71 Da        Isoelectric Point: 9.9920

>NTDB_id=112719 PFLUOLIPICF7_RS04290 WP_010213233.1 938933..940141(+) (pilC) [Pseudomonas simiae strain PICF7]
MDNASTLYAWEGINRKGRRVSGQTTGHNPALVKAQLRKQGICPGHVRKKSPVLSSLAASITSTDITLFTRQLATLLKAGI
PLLQAFDIIAEGFDNRALRERVQGLKQAIEAGTSLAEALRKQPRCFDELYCNLVAAGEQAGALETLLERVAIHREKSEQL
KTRIKKAMTYPITVLVVASLVTGVLLVHVVPQFQNLFAGVDGKLPGFTLQVIGLSEFMQKAWWAVALGGLAGGLGLREAY
RRLADFRLWLDSCLLKAPVAGKLLSKSAVARFARTLSTTFAAGVPLLQALDSVAGAAGSGPFRRAIEQMRRDVATGMPLN
QSMLTSGLFPGMAIQMTAIGEESGTLDRMLEKVANHYEADVDNLVDHLTHLMEPLIMVVLGGIVGALVIAMYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=112719 PFLUOLIPICF7_RS04290 WP_010213233.1 938933..940141(+) (pilC) [Pseudomonas simiae strain PICF7]
ATGGACAACGCTTCAACGCTCTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTCCGGGCAAACCACCGGACA
CAACCCTGCCCTGGTGAAGGCACAGTTGCGAAAACAGGGCATCTGCCCAGGGCATGTGCGCAAAAAATCCCCAGTGCTGT
CGAGCCTGGCAGCCTCGATCACATCAACCGACATCACCCTGTTCACTCGCCAACTGGCCACCCTGCTGAAGGCCGGTATT
CCGCTGCTGCAGGCCTTCGACATCATCGCTGAGGGCTTCGACAACCGCGCCTTGCGTGAGCGTGTGCAAGGGTTGAAACA
GGCCATCGAAGCCGGCACCAGCCTTGCCGAGGCGCTGCGCAAGCAGCCGCGTTGTTTCGACGAGCTGTACTGCAACCTGG
TGGCCGCCGGCGAACAGGCCGGAGCACTTGAAACCTTGCTGGAGCGCGTGGCGATCCACCGGGAAAAGAGCGAGCAGCTA
AAGACGAGGATCAAAAAAGCCATGACCTATCCGATCACCGTGCTGGTGGTCGCCAGCCTCGTCACCGGCGTGCTGCTGGT
CCACGTCGTGCCACAGTTCCAGAACCTGTTTGCCGGGGTCGACGGCAAGCTGCCGGGTTTCACCCTGCAGGTCATCGGCC
TTTCCGAGTTCATGCAGAAAGCCTGGTGGGCCGTGGCGCTGGGCGGTCTTGCGGGAGGCCTGGGGCTGCGCGAAGCGTAT
CGGCGCTTGGCGGATTTCCGTCTATGGCTGGACAGCTGTTTGTTGAAAGCGCCCGTGGCAGGCAAACTGCTGAGCAAATC
CGCCGTGGCCCGCTTCGCCCGCACGCTGTCCACCACGTTTGCCGCCGGCGTTCCGTTACTGCAGGCGCTGGATTCTGTGG
CCGGTGCCGCCGGTTCCGGGCCATTCAGACGGGCAATCGAACAGATGCGACGCGATGTAGCGACTGGCATGCCATTGAAC
CAATCCATGCTCACCAGCGGCCTGTTCCCCGGCATGGCGATCCAGATGACAGCCATCGGCGAAGAGTCGGGCACACTGGA
CCGCATGCTGGAAAAGGTCGCCAACCATTACGAGGCCGATGTGGACAACCTGGTCGATCACCTCACCCACCTCATGGAGC
CCCTGATCATGGTGGTCCTGGGAGGCATTGTCGGCGCATTGGTGATCGCCATGTACCTGCCAGTCTTCCAGTTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(68-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1N7UA21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

60.453

98.756

0.597
  pilC Acinetobacter baumannii D1279779

53.418

98.259

0.525
  pilC Acinetobacter baylyi ADP1

51.768

98.507

0.51
  pilC Legionella pneumophila strain ERS1305867

50.253

98.507

0.495
  pilC Vibrio cholerae strain A1552

41.414

98.507

0.408
  pilG Neisseria gonorrhoeae MS11

41.667

95.522

0.398
  pilC Vibrio campbellii strain DS40M4

40.102

98.01

0.393
  pilG Neisseria meningitidis 44/76-A

40.838

95.025

0.388
  pilC Thermus thermophilus HB27

36.341

99.254

0.361

Multiple sequence alignment