Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FGK83_RS03710 Genome accession   NZ_LR590465
Coordinates   738966..740360 (-) Length   464 a.a.
NCBI ID   WP_138171687.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC8468     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 733966..745360
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FGK83_RS03675 (NCTC8468_00726) - 734216..734422 (-) 207 WP_005666693.1 heavy-metal-associated domain-containing protein -
  FGK83_RS03680 (NCTC8468_00727) - 734496..734702 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  FGK83_RS03685 (NCTC8468_00728) cueR 734779..735165 (+) 387 WP_005648963.1 Cu(I)-responsive transcriptional regulator -
  FGK83_RS03690 (NCTC8468_00729) metJ 735179..735496 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  FGK83_RS03695 (NCTC8468_00730) rho 735744..737006 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  FGK83_RS03700 (NCTC8468_00731) pilD 737060..737752 (-) 693 WP_105877453.1 prepilin peptidase Machinery gene
  FGK83_RS03705 (NCTC8468_00732) pilC 737749..738969 (-) 1221 WP_138171685.1 type II secretion system F family protein Machinery gene
  FGK83_RS03710 (NCTC8468_00733) pilB 738966..740360 (-) 1395 WP_138171687.1 GspE/PulE family protein Machinery gene
  FGK83_RS03715 (NCTC8468_00734) pilA 740357..740806 (-) 450 WP_110432056.1 prepilin peptidase-dependent pilin Machinery gene
  FGK83_RS03720 (NCTC8468_00735) ampD 740920..741474 (+) 555 WP_110432055.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FGK83_RS03725 (NCTC8468_00736) corC 742106..743005 (+) 900 WP_138171689.1 CNNM family magnesium/cobalt transport protein CorC -
  FGK83_RS03730 (NCTC8468_00737) lnt 743028..744557 (+) 1530 WP_138171691.1 apolipoprotein N-acyltransferase -
  FGK83_RS03735 (NCTC8468_00738) rsmE 744607..745344 (+) 738 WP_005691816.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 464 a.a.        Molecular weight: 52852.35 Da        Isoelectric Point: 5.6536

>NTDB_id=1126751 FGK83_RS03710 WP_138171687.1 738966..740360(-) (pilB) [Haemophilus influenzae strain NCTC8468]
MTSYALLHTQRVIAQNGEVFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPCQMQVEEQVKFYQHQETHFEQENDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFSELG
MTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFNRLLR
AFLRQDPDIIMLGEIRDEESAMIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCNQGYRGRIGVYQFLHWQQNGYQTDFKNLHASGLEKVSQGITDEKEIERVLGKNS

Nucleotide


Download         Length: 1395 bp        

>NTDB_id=1126751 FGK83_RS03710 WP_138171687.1 738966..740360(-) (pilB) [Haemophilus influenzae strain NCTC8468]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAATTGCTCAAAATGGCGAGGTATTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTTTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTTTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAGCAACTTACTCCGTGCCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAAATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGGCGATTTCAATTTAAAACGACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCAGTAGAACTTAGCTTTTCTGAATTAGGT
ATGACAGAAAATCAGCAACAAGCATTTCAACGTGCGCTTAGCCAGCCACAAGGATTAATTTTAGTCACTGGTCCAACAGG
AAGTGGAAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACTGCTGAAGATC
CCATTGAAATTGAGCTGGATGGCATTATTCAAAGCCAAATTAACCCACAGATTGGATTAGATTTTAACCGCCTATTGCGC
GCTTTTTTACGCCAAGATCCCGATATTATTATGTTAGGCGAAATTCGCGATGAAGAAAGTGCAATGATTGCACTACGTGC
CGCCCAAACCGGGCATTTAGTACTCTCAACTTTACATACCAATGATGCGATATCTGCCATTTCTCGATTACAACAACTTG
GTATTCAACAGTATGAAATTGAAAACAGCTTACTGCTCGTTATTGCGCAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCAATCAAGGTTATCGAGGTCGAATCGGCGTGTATCAATTTCTACATTGGCA
GCAGAATGGCTATCAAACGGATTTTAAAAATTTACATGCGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGCTAGGTAAAAACTCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(128-395)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(29-95)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

97.198

100

0.972
  pilB Haemophilus influenzae Rd KW20

97.192

99.784

0.97
  pilB Glaesserella parasuis strain SC1401

57.391

99.138

0.569
  pilB Vibrio cholerae strain A1552

39.089

100

0.444
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.416
  pilB Legionella pneumophila strain ERS1305867

38.742

100

0.412
  pilB Acinetobacter baylyi ADP1

38.105

100

0.407
  pilB Acinetobacter baumannii D1279779

38.351

100

0.401
  pilF Neisseria gonorrhoeae MS11

36.345

100

0.373
  pilF Thermus thermophilus HB27

36.345

100

0.373
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.966

100

0.373

Multiple sequence alignment