Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSYRB_RS04210 Genome accession   NZ_CP005969
Coordinates   942220..943437 (-) Length   405 a.a.
NCBI ID   WP_032654583.1    Uniprot ID   A0A3M5X361
Organism   Pseudomonas syringae pv. syringae B301D     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 937220..948437
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSYRB_RS04180 (PsyrB_04225) - 938646..939179 (-) 534 WP_032654590.1 MOSC domain-containing protein -
  PSYRB_RS04185 (PsyrB_04230) - 939176..939805 (-) 630 WP_003345130.1 DUF1780 domain-containing protein -
  PSYRB_RS04190 (PsyrB_04235) - 939932..940393 (+) 462 WP_003406557.1 hypothetical protein -
  PSYRB_RS04195 (PsyrB_04240) yacG 940520..940729 (-) 210 WP_002552020.1 DNA gyrase inhibitor YacG -
  PSYRB_RS04200 (PsyrB_04245) coaE 940726..941349 (-) 624 WP_032654586.1 dephospho-CoA kinase -
  PSYRB_RS04205 (PsyrB_04250) pilD 941346..942218 (-) 873 WP_003406561.1 A24 family peptidase Machinery gene
  PSYRB_RS04210 (PsyrB_04255) pilC 942220..943437 (-) 1218 WP_032654583.1 type II secretion system F family protein Machinery gene
  PSYRB_RS04215 (PsyrB_04260) pilB 943440..945134 (-) 1695 WP_003406565.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSYRB_RS04220 (PsyrB_04265) - 945358..945786 (+) 429 WP_003406568.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  PSYRB_RS30010 - 946087..946194 (-) 108 Protein_834 transcriptional regulator -
  PSYRB_RS29650 (PsyrB_04270) - 946360..946776 (+) 417 WP_225650732.1 glutathione binding-like protein -
  PSYRB_RS04230 (PsyrB_04275) - 946873..947418 (-) 546 WP_003317774.1 DUF1523 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44033.01 Da        Isoelectric Point: 10.1950

>NTDB_id=112665 PSYRB_RS04210 WP_032654583.1 942220..943437(-) (pilC) [Pseudomonas syringae pv. syringae B301D]
MASKAIKVIVYTWEGVDKKGAKTSGELSGHNLALVKAQLRKQGINPTKVRKKTVSIFGKGKKIKPLDIAFFARQMATMMK
AGVPLLQSFDIIGEGAENPNMRALVSSLKQEVSAGNSFAMALRQKPEYFDDLFCNLVDAGEQAGALESLLDRVASYKEKT
EKLKAKIKKAMTYPAAVLIVAVIVSGILLIKVVPQFQSVFAGFGAELPTFTLMVIGLSEVVQKWWLAIVGLFFASVFIFK
RAYKRSQKFRDSLDRFLLKIPVIGPLIFKSSVARYARTLATTFAAGVPLVEALDSVAGATGNVVFRNAVNKVKQDVSTGM
QLNFSMRSTGVFPSLAIQMTAIGEESGALDNMLDKVATYYEDEVDNMVDSLTSLMEPMIMAVLGVVVGGLVIAMYLPIFK
LGDVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=112665 PSYRB_RS04210 WP_032654583.1 942220..943437(-) (pilC) [Pseudomonas syringae pv. syringae B301D]
ATGGCCAGCAAGGCAATCAAAGTCATCGTTTACACGTGGGAAGGTGTCGACAAGAAAGGCGCTAAAACCAGTGGGGAATT
GAGTGGCCACAACCTAGCACTTGTAAAAGCGCAACTGCGCAAACAAGGCATCAACCCAACCAAGGTACGTAAAAAAACAG
TTTCGATCTTTGGCAAGGGTAAGAAGATCAAGCCGCTGGATATCGCTTTTTTCGCTCGACAGATGGCAACGATGATGAAA
GCAGGAGTTCCCTTATTACAATCGTTCGACATCATCGGTGAGGGTGCAGAAAATCCAAATATGCGGGCGCTGGTGAGTTC
ACTCAAACAGGAGGTATCAGCAGGCAACAGCTTTGCAATGGCTTTACGGCAGAAGCCTGAGTATTTCGATGATCTTTTTT
GCAATCTCGTGGATGCCGGCGAGCAAGCAGGAGCTCTGGAGAGTTTGCTGGATCGAGTAGCGAGCTACAAAGAAAAAACA
GAGAAGCTCAAAGCAAAAATAAAGAAAGCAATGACCTACCCTGCCGCTGTGTTAATCGTGGCGGTCATCGTATCTGGCAT
TCTATTAATCAAAGTAGTCCCGCAGTTTCAGTCAGTATTCGCAGGATTCGGCGCCGAGCTACCTACATTCACTTTGATGG
TCATAGGACTTTCAGAAGTAGTTCAGAAGTGGTGGCTGGCGATTGTAGGCCTGTTTTTCGCTAGCGTCTTCATATTCAAG
CGCGCGTATAAGCGATCACAAAAATTTCGCGATAGCCTAGATCGTTTTTTACTTAAAATACCCGTCATTGGGCCGCTGAT
TTTCAAATCCTCAGTGGCGCGATATGCTCGTACTCTTGCAACTACTTTTGCGGCTGGGGTTCCTCTAGTAGAGGCTCTCG
ACTCTGTTGCAGGAGCAACCGGCAATGTAGTTTTCAGAAATGCAGTTAACAAAGTCAAGCAAGATGTATCCACAGGCATG
CAACTCAACTTCTCAATGCGATCAACCGGAGTTTTCCCGAGCTTGGCAATTCAGATGACTGCGATCGGAGAGGAGTCCGG
TGCTCTGGACAACATGTTGGACAAGGTCGCGACTTACTATGAGGATGAAGTCGACAATATGGTTGATAGCCTGACCAGCC
TAATGGAGCCCATGATCATGGCCGTCCTTGGGGTAGTAGTTGGCGGTCTAGTAATTGCGATGTACTTGCCAATATTCAAA
CTCGGGGATGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3M5X361

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

75.556

100

0.756
  pilC Acinetobacter baylyi ADP1

59.559

100

0.6
  pilC Acinetobacter baumannii D1279779

59.406

99.753

0.593
  pilC Legionella pneumophila strain ERS1305867

52.13

98.519

0.514
  pilG Neisseria gonorrhoeae MS11

43.719

98.272

0.43
  pilG Neisseria meningitidis 44/76-A

43.467

98.272

0.427
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

42.569

98.025

0.417
  pilC Thermus thermophilus HB27

39.152

99.012

0.388

Multiple sequence alignment