Detailed information NTG0112664

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PSYRB_RS04205
• Genome accession: NZ_CP005969
• Coordinates: 941346..942218 (-)
• Length: 290 a.a.
• NCBI ID: WP_003406561.1
• Uniprot ID: -
• Organism: Pseudomonas syringae pv. syringae B301D
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 936346..947218

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSYRB_RS04175 (PsyrB_04220)	-	937131..938429 (-)	1299	WP_003392712.1	NAD(P)/FAD-dependent oxidoreductase	-
PSYRB_RS04180 (PsyrB_04225)	-	938646..939179 (-)	534	WP_032654590.1	MOSC domain-containing protein	-
PSYRB_RS04185 (PsyrB_04230)	-	939176..939805 (-)	630	WP_003345130.1	DUF1780 domain-containing protein	-
PSYRB_RS04190 (PsyrB_04235)	-	939932..940393 (+)	462	WP_003406557.1	hypothetical protein	-
PSYRB_RS04195 (PsyrB_04240)	yacG	940520..940729 (-)	210	WP_002552020.1	DNA gyrase inhibitor YacG	-
PSYRB_RS04200 (PsyrB_04245)	coaE	940726..941349 (-)	624	WP_032654586.1	dephospho-CoA kinase	-
PSYRB_RS04205 (PsyrB_04250)	pilD	941346..942218 (-)	873	WP_003406561.1	A24 family peptidase	Machinery gene
PSYRB_RS04210 (PsyrB_04255)	pilC	942220..943437 (-)	1218	WP_032654583.1	type II secretion system F family protein	Machinery gene
PSYRB_RS04215 (PsyrB_04260)	pilB	943440..945134 (-)	1695	WP_003406565.1	type IV-A pilus assembly ATPase PilB	Machinery gene
PSYRB_RS04220 (PsyrB_04265)	-	945358..945786 (+)	429	WP_003406568.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
PSYRB_RS30010	-	946087..946194 (-)	108	Protein_834	transcriptional regulator	-
PSYRB_RS29650 (PsyrB_04270)	-	946360..946776 (+)	417	WP_225650732.1	glutathione binding-like protein	-

Sequence

Protein

• Length: 290 a.a.
• Molecular weight: 31983.15 Da
• Isoelectric Point: 8.3734

>NTDB_id=112664 PSYRB_RS04205 WP_003406561.1 941346..942218(-) (pilD) [Pseudomonas syringae pv. syringae B301D]
MSLLDLLASSPLAFVTTCCILGLIIGSFLNVIVYRLPIMMERDWKAQSRELLGLPAEPDQPVFNLNRPRSSCPHCAHKIR
PWENLPVISYLLLRGKCSQCKAPISKRYPLVELTCAVLSAYVAWHFGFGWQAAAMLVLGWGLLAMSLIDADHQLLPDSLV
LPLLWLGLIVNAFGLFTSLNDALWGAVAGYLALWSVFWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVG
AVLGVIMMRVRRVESGTPIPFGPYLAIAGWIALLWGGQITDSYMQFAGFR

Nucleotide

• Length: 873 bp

>NTDB_id=112664 PSYRB_RS04205 WP_003406561.1 941346..942218(-) (pilD) [Pseudomonas syringae pv. syringae B301D]
ATGTCCCTCCTCGACCTCCTGGCCAGCTCACCCCTGGCCTTCGTCACCACCTGCTGCATTCTCGGCCTGATCATCGGAAG
CTTCCTCAACGTGATCGTCTATCGCCTGCCAATAATGATGGAGCGCGACTGGAAAGCCCAATCCCGAGAACTGCTCGGCC
TGCCTGCTGAACCCGATCAACCCGTCTTCAATCTGAACCGCCCCCGTTCCAGCTGCCCGCATTGCGCACACAAAATCCGC
CCTTGGGAGAACCTGCCGGTGATCAGCTATCTGCTGCTGCGTGGTAAATGCTCGCAGTGCAAAGCGCCGATCAGCAAACG
CTACCCGCTGGTCGAGCTGACCTGCGCAGTGTTGTCGGCCTATGTGGCTTGGCATTTCGGTTTTGGCTGGCAGGCGGCGG
CGATGCTGGTATTGGGCTGGGGGCTGCTGGCGATGAGTCTGATCGATGCTGATCACCAGCTGTTACCTGACTCTCTGGTG
CTGCCACTGCTGTGGCTGGGCCTGATCGTCAACGCTTTCGGGCTGTTTACCTCGCTGAACGATGCGCTGTGGGGAGCGGT
GGCCGGCTATCTGGCGCTGTGGTCGGTGTTCTGGCTGTTCAAGCTGGTGACCGGCAAGGAAGGCATGGGTTATGGCGACT
TCAAGTTGCTGGCCATGCTGGGCGCGTGGGGCGGCTGGCAGATTTTGCCATTGACCATCCTGTTATCGTCGCTGGTGGGC
GCGGTGCTCGGGGTGATCATGATGCGTGTGCGCAGGGTCGAGAGCGGTACGCCGATCCCCTTTGGTCCTTATCTGGCCAT
CGCGGGGTGGATCGCTTTGCTCTGGGGTGGTCAAATAACCGACTCTTACATGCAGTTTGCCGGTTTCAGATGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (136-245)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-126)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	52.65	97.586	0.514
pilD	Vibrio campbellii strain DS40M4	51.943	97.586	0.507
pilD	Acinetobacter baumannii D1279779	52.19	94.483	0.493
pilD	Acinetobacter nosocomialis M2	51.46	94.483	0.486
pilD	Neisseria gonorrhoeae MS11	51.119	92.414	0.472