Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   EL297_RS00215 Genome accession   NZ_LR134525
Coordinates   30880..32112 (-) Length   410 a.a.
NCBI ID   WP_002248468.1    Uniprot ID   -
Organism   Neisseria meningitidis strain NCTC10025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 25880..37112
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL297_RS00180 (NCTC10025_00028) - 26461..26907 (+) 447 WP_002233503.1 DUF441 domain-containing protein -
  EL297_RS00195 (NCTC10025_00030) pilF 27349..29025 (+) 1677 WP_002221967.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL297_RS00200 (NCTC10025_00031) - 29110..29319 (-) 210 WP_002249247.1 DNA gyrase inhibitor YacG -
  EL297_RS00205 (NCTC10025_00032) coaE 29312..29944 (-) 633 WP_002226983.1 dephospho-CoA kinase -
  EL297_RS00210 (NCTC10025_00033) pilD 29946..30806 (-) 861 WP_002226036.1 prepilin peptidase Machinery gene
  EL297_RS00215 (NCTC10025_00034) pilG 30880..32112 (-) 1233 WP_002248468.1 type 4 pilus assembly protein PilG Machinery gene
  EL297_RS00225 (NCTC10025_00035) pgi 32622..34265 (+) 1644 WP_002249249.1 glucose-6-phosphate isomerase -
  EL297_RS00230 - 34235..34465 (+) 231 WP_002212338.1 hypothetical protein -
  EL297_RS00235 (NCTC10025_00036) dapD 34475..35296 (+) 822 WP_002226039.1 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase -
  EL297_RS00240 (NCTC10025_00037) fabI 35451..36236 (+) 786 WP_002223367.1 enoyl-ACP reductase FabI -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 45301.34 Da        Isoelectric Point: 9.9273

>NTDB_id=1123910 EL297_RS00215 WP_002248468.1 30880..32112(-) (pilG) [Neisseria meningitidis strain NCTC10025]
MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEVRKKLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLS
TMIKAGLPLMQAFEIVARGHGNPSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTVMDMSDFFVSYGWMVLIALGFAI
YGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTIARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRV
IQGLSMTSGMRATELFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIGTLLVAMYL
PLFNLGNVVA

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=1123910 EL297_RS00215 WP_002248468.1 30880..32112(-) (pilG) [Neisseria meningitidis strain NCTC10025]
ATGGCTAAAAACGGAGGATTTTCTTTGTTCGCAAAGAAAGAAAAACGCTTTATCTTTGAAGGCAGGCATTCCGCCTCCGA
CAAACTGGTCAACGGCGAAGTATCCGCGTTTACCGAAGAAGAGGTGCGCAAAAAACTGGCAAAACGCGGCATCCGCCCGT
TGCAGATTACCCGTGTGAAAACAAGCTCCAAGCGCAAAATCACACAAGAAGACATCACCGTTTTCACCCGCCAGCTGTCC
ACGATGATTAAAGCGGGCCTGCCGCTGATGCAGGCATTTGAAATCGTGGCGCGCGGACACGGCAACCCGTCTATGACGGA
AATGCTGATGGAAATCCGAGGCGAAGTGGAACAGGGCAGCTCGTTGAGCCGCGCATTCTCAAACCACCCAAAATATTTCG
ACCGCTTCTACTGCAATCTGGTTGCGGCGGGCGAAACGGGCGGCGTATTGGAAAGCCTGCTGGACAAATTGGCAATTTAC
AAAGAAAAAACCCAGGCCATCCGCAAAAAGGTAAAAACCGCACTGACCTATCCGGTATCCGTCATCGCCGTCGCCATCGG
TTTGGTATTCGTGATGATGATTTTCGTACTGCCCGCCTTTAAAGAAGTTTACGCCAATATGGGCGCGGAGCTTCCCGCAC
TGACCCAAACAGTGATGGATATGTCCGACTTTTTCGTCTCATACGGCTGGATGGTGCTGATCGCACTGGGCTTTGCCATA
TACGGCTTCCTTAAATTGAAGGCGCGTTCGATTAAAATCCAACGGCGTATGGATGCCATACTGCTGCGTATGCCGATTTT
CGGAGACATTGTCCGCAAAGGGACGATTGCCCGCTGGGGCAGGACGACGGCGACGCTGATTGCGGCAGGCGTGCCTTTGG
TCGATGTATTGGACTCCACTGCCGGCGCGGCGGGCAATTTAATCTATGAAGAAGCCACCCGGGAAATCCGTACGCGCGTC
ATCCAAGGTTTGTCTATGACTTCGGGGATGCGTGCGACGGAACTGTTCCCCAATATGATGTTGCAGATGTCTTCCATCGG
CGAGGAATCGGGTTCTTTGGACGATATGCTCAACAAAGCCGCCGAATTTTACGAAGACGAGGTGGACAATGCGGTCGGCA
GGCTGTCCGCTATGATGGAGCCGATCATTATCGTGATTTTAGGCTTGGTCATCGGCACGCTTCTGGTCGCCATGTATCTG
CCGCTGTTTAATTTGGGCAACGTGGTCGCCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(75-198)

Type II secretion system (T2SS), protein F (T2SSF)

(281-401)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

99.024

100

0.99
  pilG Neisseria meningitidis 44/76-A

98.293

100

0.983
  pilC Acinetobacter baumannii D1279779

47.236

97.073

0.459
  pilC Pseudomonas stutzeri DSM 10701

45.161

98.293

0.444
  pilC Acinetobacter baylyi ADP1

45.363

97.317

0.441
  pilC Legionella pneumophila strain ERS1305867

44.584

96.829

0.432
  pilC Vibrio cholerae strain A1552

41.294

98.049

0.405
  pilC Vibrio campbellii strain DS40M4

39.409

99.024

0.39

Multiple sequence alignment