Detailed information NTG1123909

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EL297_RS00210
• Genome accession: NZ_LR134525
• Coordinates: 29946..30806 (-)
• Length: 286 a.a.
• NCBI ID: WP_002226036.1
• Uniprot ID: A0A112F5U3
• Organism: Neisseria meningitidis strain NCTC10025
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 24946..35806

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL297_RS00165 (NCTC10025_00026)	rplU	24950..25258 (-)	309	WP_002216394.1	50S ribosomal protein L21	-
EL297_RS00175 (NCTC10025_00027)	ispB	25482..26456 (+)	975	WP_002216396.1	octaprenyl diphosphate synthase	-
EL297_RS00180 (NCTC10025_00028)	-	26461..26907 (+)	447	WP_002233503.1	DUF441 domain-containing protein	-
EL297_RS00195 (NCTC10025_00030)	pilF	27349..29025 (+)	1677	WP_002221967.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EL297_RS00200 (NCTC10025_00031)	-	29110..29319 (-)	210	WP_002249247.1	DNA gyrase inhibitor YacG	-
EL297_RS00205 (NCTC10025_00032)	coaE	29312..29944 (-)	633	WP_002226983.1	dephospho-CoA kinase	-
EL297_RS00210 (NCTC10025_00033)	pilD	29946..30806 (-)	861	WP_002226036.1	prepilin peptidase	Machinery gene
EL297_RS00215 (NCTC10025_00034)	pilG	30880..32112 (-)	1233	WP_002248468.1	type 4 pilus assembly protein PilG	Machinery gene
EL297_RS00225 (NCTC10025_00035)	pgi	32622..34265 (+)	1644	WP_002249249.1	glucose-6-phosphate isomerase	-
EL297_RS00230	-	34235..34465 (+)	231	WP_002212338.1	hypothetical protein	-
EL297_RS00235 (NCTC10025_00036)	dapD	34475..35296 (+)	822	WP_002226039.1	2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31389.43 Da
• Isoelectric Point: 8.2213

>NTDB_id=1123909 EL297_RS00210 WP_002226036.1 29946..30806(-) (pilD) [Neisseria meningitidis strain NCTC10025]
MSDLSVLSPFAVPLTAVFGLLVGSFLNVVIYRVPVMMERGWTVFAKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAW
QNIPIVSYLLLRGKCASCQTKISIRYPLIELLTGVLFGLVAWQYGWSWITLGGLILTAFLISLTFIDADTQYLPDSMTLP
LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAALGAWLGISALPVLIFVSSLIGLV
AAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRAVNWWLTHPVL

Nucleotide

• Length: 861 bp

>NTDB_id=1123909 EL297_RS00210 WP_002226036.1 29946..30806(-) (pilD) [Neisseria meningitidis strain NCTC10025]
ATGTCTGATTTGTCTGTATTGTCGCCGTTTGCCGTGCCTTTGACAGCGGTGTTCGGGCTGCTGGTCGGAAGTTTCTTAAA
TGTCGTCATTTACCGCGTGCCGGTCATGATGGAACGCGGCTGGACGGTATTTGCCAAAGAATATTTAAACCTGCCGCTGA
CCGAAGAGGAAAGCCGTACCTTCAACCTGATGAAACCGGATTCCTGCTGTCCCAAATGCCGCGTGCCGATACGCGCGTGG
CAGAACATCCCGATTGTCAGCTACCTGCTCCTGCGCGGCAAATGCGCCTCCTGCCAAACCAAAATCAGCATACGCTATCC
CTTAATCGAACTGCTGACCGGCGTATTGTTCGGGCTGGTTGCCTGGCAATACGGCTGGTCTTGGATTACATTGGGCGGTT
TGATACTGACCGCGTTTCTGATTTCCCTGACCTTTATCGATGCGGACACCCAATACCTGCCCGACTCGATGACATTACCC
TTGATTTGGCTGGGGCTGATATTTAATTTGGACGGCGGCTTCGTGCCTTTGCAGTCTGCCGTTTTAGGTGCGGTTGCCGG
CTATGGTTCGATATGGCTTTTATGCGCGGTATATAAACTGATTAAAGGCGAAATCGGTATGGGCAACGGAGATTTCAAAC
TGATTGCCGCATTGGGCGCGTGGCTCGGCATATCCGCATTGCCCGTGCTGATTTTTGTTTCCTCGCTGATCGGTTTGGTC
GCGGCAATCGTTATGCGCGTCGCCAAGGGGCAGCATTTTGCCTTCGGCCCCGCACTGACAGTTTCGGGCTGGATAATTTT
TACAGCAAACGATTCCGTATGGCGGGCGGTCAACTGGTGGCTGACCCATCCGGTGCTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (134-242)

3D structure

• AlphaFold DB: A0A112F5U3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Neisseria gonorrhoeae MS11	95.789	99.65	0.955
pilD	Vibrio cholerae strain A1552	44.097	100	0.444
pilD	Acinetobacter baumannii D1279779	46.97	92.308	0.434
pilD	Acinetobacter nosocomialis M2	46.97	92.308	0.434
pilD	Vibrio campbellii strain DS40M4	44.565	96.503	0.43