Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL297_RS00195 Genome accession   NZ_LR134525
Coordinates   27349..29025 (+) Length   558 a.a.
NCBI ID   WP_002221967.1    Uniprot ID   A0A0Y5AAS9
Organism   Neisseria meningitidis strain NCTC10025     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 22349..34025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL297_RS00140 (NCTC10025_00022) rpmB 22439..22672 (+) 234 WP_002216391.1 50S ribosomal protein L28 -
  EL297_RS00145 (NCTC10025_00023) rpmG 22704..22859 (+) 156 WP_002212306.1 50S ribosomal protein L33 -
  EL297_RS00155 (NCTC10025_00024) ubiM 23130..24314 (-) 1185 WP_002239495.1 5-demethoxyubiquinol-8 5-hydroxylase UbiM -
  EL297_RS14035 - 24478..24600 (-) 123 Protein_20 IS5/IS1182 family transposase -
  EL297_RS00160 (NCTC10025_00025) rpmA 24653..24925 (-) 273 WP_002212328.1 50S ribosomal protein L27 -
  EL297_RS00165 (NCTC10025_00026) rplU 24950..25258 (-) 309 WP_002216394.1 50S ribosomal protein L21 -
  EL297_RS00175 (NCTC10025_00027) ispB 25482..26456 (+) 975 WP_002216396.1 octaprenyl diphosphate synthase -
  EL297_RS00180 (NCTC10025_00028) - 26461..26907 (+) 447 WP_002233503.1 DUF441 domain-containing protein -
  EL297_RS00195 (NCTC10025_00030) pilF 27349..29025 (+) 1677 WP_002221967.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL297_RS00200 (NCTC10025_00031) - 29110..29319 (-) 210 WP_002249247.1 DNA gyrase inhibitor YacG -
  EL297_RS00205 (NCTC10025_00032) coaE 29312..29944 (-) 633 WP_002226983.1 dephospho-CoA kinase -
  EL297_RS00210 (NCTC10025_00033) pilD 29946..30806 (-) 861 WP_002226036.1 prepilin peptidase Machinery gene
  EL297_RS00215 (NCTC10025_00034) pilG 30880..32112 (-) 1233 WP_002248468.1 type 4 pilus assembly protein PilG Machinery gene

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61965.05 Da        Isoelectric Point: 5.3037

>NTDB_id=1123908 EL297_RS00195 WP_002221967.1 27349..29025(+) (pilF) [Neisseria meningitidis strain NCTC10025]
MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVISPKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQ
MVEFHCVPVFRRGDKVFFAVSDPTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPIAVRGQLASRIKVMSRLDISEKR
IPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMRILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSG
KTVSLYTCLNILNTESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETADIAIKAAQ
TGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSSCKQEVERPSASALKEVGFTDEDLAKDWKLY
RAVGCDRCRGQGYKGRAGVYEVMPISEEMQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1123908 EL297_RS00195 WP_002221967.1 27349..29025(+) (pilF) [Neisseria meningitidis strain NCTC10025]
ATGAGCGTAGGTTTGCTGAGGATTCTGGTTCAAAACCAGGTGGTTACTGTTGAGCAGGCCGAGCATTACTACAATGAGTC
GCAGGCGGGTAAGGAAGTGTTGCCGATGCTGTTTTCAGACGGTGTCATTTCGCCCAAGTCGCTTGCGGCATTGATTGCGA
GGGTGTTCAGTTATTCGATTCTTGATTTGCGTCATTATCCGCGCCACAGGGTGCTGATGGGGGTGTTGACGGAGGAGCAG
ATGGTGGAGTTCCACTGTGTGCCGGTTTTCCGTCGGGGCGACAAAGTATTTTTTGCGGTTTCCGATCCGACACAGATGCC
GCAAATTCAGAAAACCGTTTCTGCCGCAGGGATTGAGGTTGAGTTGGTCATTGTCGAGGATGACCAGTTGGCGGGTTTGC
TCGATTGGGTGGGTTCGCGTTCGACATCGCTGCTTCAGGAGCTTGGGGAGGGGCAGGAGGAAGAGGAAAGCCACACCCTG
TATATCGACAACGAGGAGGCAGAAGACGGCCCTGTTCCGAGGTTTATCCATAAGACTTTGTCGGATGCCTTGCGCAGCGG
GGCATCGGACATCCATTTCGAGTTTTACGAACACAATGCCCGTATCCGTTTCCGTGTGGACGGGCAGCTCCGCGAGGTGG
TTCAGCCGCCCATTGCGGTAAGGGGGCAGCTTGCTTCCCGGATTAAGGTAATGTCGCGTTTGGACATTTCCGAAAAACGG
ATACCGCAGGACGGCAGGATGCAGCTGACCTTTCAAAAGGGCGGCAAGCCTGTCGATTTCCGTGTCAGCACATTGCCGAC
GCTGTTTGGCGAAAAGGTCGTGATGCGGATTTTGAATTCCGATGCCGCGTCTTTGAACATCGACCAGCTCGGTTTTGAGC
CGTTTCAGAAAAAATTGTTGTTGGAAGCGATTCACCGTCCCTACGGGATGGTGCTGGTAACCGGTCCGACGGGTTCGGGT
AAGACGGTGTCGCTCTATACCTGTTTGAATATTTTGAATACGGAGTCGGTAAATATTGCAACGGCGGAAGACCCTGCCGA
GATTAACCTGCCGGGCATCAATCAGGTTAACGTCAATGATAAGCAGGGTCTGACTTTTGCCGCTGCTTTGAAGTCTTTCC
TGCGTCAGGACCCGGACATCATTATGGTCGGTGAGATTCGTGATTTGGAAACTGCCGATATTGCGATTAAGGCGGCACAA
ACAGGGCATATGGTGTTTTCCACCCTGCACACCAATAATGCGCCGGCGACGTTGTCGCGTATGCTGAATATGGGTGTCGC
GCCGTTTAATATTGCCAGTTCGGTCAGCCTGATTATGGCGCAGCGTCTTTTACGCAGGCTGTGTTCGAGCTGCAAACAGG
AAGTGGAACGCCCGTCTGCCTCTGCTTTGAAGGAAGTCGGCTTCACCGATGAGGACCTTGCAAAAGATTGGAAACTTTAC
CGCGCCGTCGGTTGCGACCGTTGCCGGGGGCAGGGTTATAAGGGGCGTGCGGGCGTGTATGAGGTTATGCCCATCAGCGA
AGAAATGCAGCGTGTGATTATGAACAACGGTACGGAAGTGGATATTTTGGACGTTGCCTATAAGGAGGGTATGGTGGATT
TGCGCCGGGCCGGTATTTTGAAAGTTATGCAGGGCATTACTTCATTGGAAGAGGTAACGGCAAATACCAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-446)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Y5AAS9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

99.462

100

0.995
  pilB Acinetobacter baumannii D1279779

50.265

100

0.511
  pilB Acinetobacter baylyi ADP1

50.356

100

0.507
  pilB Legionella pneumophila strain ERS1305867

47.527

100

0.482
  pilB Vibrio parahaemolyticus RIMD 2210633

45.39

100

0.459
  pilB Vibrio cholerae strain A1552

45.374

100

0.457
  pilB Vibrio campbellii strain DS40M4

43.838

100

0.446
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.728

0.375
  pilF Thermus thermophilus HB27

36.949

97.491

0.36

Multiple sequence alignment