Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EL287_RS04315 Genome accession   NZ_LR134393
Coordinates   890819..892030 (-) Length   403 a.a.
NCBI ID   WP_048378219.1    Uniprot ID   A0A0J6GQ82
Organism   Pseudomonas taetrolens strain NCTC8067     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 885819..897030
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL287_RS04280 (NCTC8067_00858) - 886863..887036 (-) 174 WP_048378226.1 DUF3094 family protein -
  EL287_RS04285 (NCTC8067_00859) - 887092..887619 (-) 528 WP_048378225.1 MOSC domain-containing protein -
  EL287_RS04290 (NCTC8067_00860) - 887616..888245 (-) 630 WP_048378224.1 DUF1780 domain-containing protein -
  EL287_RS04295 (NCTC8067_00861) - 888377..889063 (+) 687 WP_048378223.1 energy-coupling factor ABC transporter permease -
  EL287_RS04300 (NCTC8067_00862) yacG 889131..889334 (-) 204 WP_048378222.1 DNA gyrase inhibitor YacG -
  EL287_RS04305 (NCTC8067_00863) coaE 889331..889954 (-) 624 WP_048378221.1 dephospho-CoA kinase -
  EL287_RS04310 (NCTC8067_00864) pilD 889956..890816 (-) 861 WP_048378220.1 prepilin peptidase Machinery gene
  EL287_RS04315 (NCTC8067_00865) pilC 890819..892030 (-) 1212 WP_048378219.1 type II secretion system F family protein Machinery gene
  EL287_RS04320 (NCTC8067_00866) - 892254..892661 (+) 408 WP_048378218.1 pilin -
  EL287_RS04325 (NCTC8067_00867) - 892832..894322 (+) 1491 WP_048378217.1 DNA-3-methyladenine glycosylase 2 family protein -
  EL287_RS04330 (NCTC8067_00868) - 894325..894822 (+) 498 WP_048378216.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  EL287_RS04335 (NCTC8067_00869) nadC 894877..895725 (-) 849 WP_048378215.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EL287_RS04340 (NCTC8067_00870) ampD 895935..896498 (+) 564 WP_048378214.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 403 a.a.        Molecular weight: 43916.91 Da        Isoelectric Point: 9.9104

>NTDB_id=1122654 EL287_RS04315 WP_048378219.1 890819..892030(-) (pilC) [Pseudomonas taetrolens strain NCTC8067]
MATLDVKTCLYRWEGTDRKGKRIKGEMAAHSCAMIRMHLRRQGITATRIRREGRSRAGTPVTALHITLFTRQLATLIRAG
IPLLQALEVISNGFETLRMRQLVADLRQDIGAGTSLAAALRKKPLHFDALFCSLIEAGEQAGALDALLERIAIYKEKTEA
LKKKIKKAMTYPVAVLLVALAVCTILLIEVVPKFQGIFSTFDAELPAFTLGAIALSEALKTCGLWLLAGVCATLGCARRY
YRHSATWRQRLDRWQLKLPVTGRLLHQSAIARFARTLSITMAAGVPLLEALDSVAGATGNSVFKEAVLRLKQQVATGSQI
HMAMTSAGVFPGMAIQMTAIGEESGTLDDMLGHVATYYENEVDTLVDNLTTLMEPAIMVVLGVLVGGLVIAMYLPIFQLG
QVI

Nucleotide


Download         Length: 1212 bp        

>NTDB_id=1122654 EL287_RS04315 WP_048378219.1 890819..892030(-) (pilC) [Pseudomonas taetrolens strain NCTC8067]
ATGGCAACACTGGACGTTAAAACCTGCCTGTACCGGTGGGAAGGTACTGATCGCAAAGGCAAACGGATCAAGGGGGAAAT
GGCCGCTCATTCTTGCGCCATGATCCGGATGCACCTGCGTCGCCAGGGCATCACTGCGACGAGGATCCGACGCGAGGGCA
GGTCCCGCGCTGGAACACCCGTCACGGCGCTGCATATCACGCTGTTCACGCGCCAATTGGCGACTCTGATCAGGGCTGGC
ATCCCGCTGCTGCAAGCGCTGGAGGTGATCAGTAACGGCTTCGAAACGCTGCGGATGCGCCAACTGGTGGCTGACCTCAG
GCAGGACATCGGCGCAGGTACCAGCCTGGCGGCGGCGCTGCGCAAAAAACCGCTGCACTTTGATGCTCTGTTTTGCAGCC
TTATCGAAGCCGGTGAACAGGCCGGCGCGCTGGATGCACTGCTTGAACGGATCGCGATCTACAAAGAGAAAACCGAAGCG
CTTAAAAAGAAAATCAAAAAGGCAATGACCTACCCCGTCGCGGTTCTGCTGGTGGCGTTGGCGGTATGTACGATTCTGCT
GATCGAAGTGGTGCCGAAGTTTCAGGGCATTTTCAGCACATTCGATGCTGAACTTCCCGCCTTCACGCTCGGCGCAATTG
CCTTGTCAGAAGCCCTGAAAACCTGTGGGCTGTGGCTGCTGGCAGGTGTTTGCGCCACACTGGGCTGTGCGCGCCGCTAC
TACAGACACTCTGCGACCTGGCGCCAACGGCTTGATCGGTGGCAATTAAAACTGCCCGTGACAGGGCGCCTGCTTCACCA
GTCAGCCATTGCCCGTTTTGCTCGCACGCTGTCCATCACAATGGCAGCCGGCGTTCCTCTGCTCGAGGCGCTGGACTCCG
TGGCCGGCGCCACTGGCAATAGCGTGTTCAAGGAGGCAGTGCTGCGACTCAAGCAACAGGTGGCGACGGGCAGCCAGATT
CACATGGCCATGACCAGCGCAGGCGTGTTTCCGGGCATGGCGATTCAGATGACAGCTATTGGCGAAGAATCTGGCACATT
GGACGACATGCTTGGTCATGTAGCCACTTATTATGAGAACGAGGTCGACACTTTGGTCGATAACCTGACAACGCTGATGG
AGCCCGCCATCATGGTGGTGCTTGGCGTTCTGGTAGGCGGGCTGGTCATTGCCATGTATTTACCGATTTTCCAACTGGGC
CAAGTGATTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-395)

Type II secretion system (T2SS), protein F (T2SSF)

(69-192)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0J6GQ82

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

55.309

100

0.556
  pilC Acinetobacter baumannii D1279779

50.63

98.511

0.499
  pilC Acinetobacter baylyi ADP1

49.497

98.759

0.489
  pilC Legionella pneumophila strain ERS1305867

47.88

99.504

0.476
  pilC Vibrio cholerae strain A1552

40.594

100

0.407
  pilC Vibrio campbellii strain DS40M4

38.424

100

0.387
  pilG Neisseria meningitidis 44/76-A

39.793

96.03

0.382
  pilC Thermus thermophilus HB27

37.343

99.007

0.37
  pilG Neisseria gonorrhoeae MS11

38.903

95.037

0.37

Multiple sequence alignment