Detailed information NTG1122653

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EL287_RS04310
• Genome accession: NZ_LR134393
• Coordinates: 889956..890816 (-)
• Length: 286 a.a.
• NCBI ID: WP_048378220.1
• Uniprot ID: -
• Organism: Pseudomonas taetrolens strain NCTC8067
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 884956..895816

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EL287_RS04275 (NCTC8067_00857)	-	885498..886799 (-)	1302	WP_048378227.1	NAD(P)/FAD-dependent oxidoreductase	-
EL287_RS04280 (NCTC8067_00858)	-	886863..887036 (-)	174	WP_048378226.1	DUF3094 family protein	-
EL287_RS04285 (NCTC8067_00859)	-	887092..887619 (-)	528	WP_048378225.1	MOSC domain-containing protein	-
EL287_RS04290 (NCTC8067_00860)	-	887616..888245 (-)	630	WP_048378224.1	DUF1780 domain-containing protein	-
EL287_RS04295 (NCTC8067_00861)	-	888377..889063 (+)	687	WP_048378223.1	energy-coupling factor ABC transporter permease	-
EL287_RS04300 (NCTC8067_00862)	yacG	889131..889334 (-)	204	WP_048378222.1	DNA gyrase inhibitor YacG	-
EL287_RS04305 (NCTC8067_00863)	coaE	889331..889954 (-)	624	WP_048378221.1	dephospho-CoA kinase	-
EL287_RS04310 (NCTC8067_00864)	pilD	889956..890816 (-)	861	WP_048378220.1	prepilin peptidase	Machinery gene
EL287_RS04315 (NCTC8067_00865)	pilC	890819..892030 (-)	1212	WP_048378219.1	type II secretion system F family protein	Machinery gene
EL287_RS04320 (NCTC8067_00866)	-	892254..892661 (+)	408	WP_048378218.1	pilin	-
EL287_RS04325 (NCTC8067_00867)	-	892832..894322 (+)	1491	WP_048378217.1	DNA-3-methyladenine glycosylase 2 family protein	-
EL287_RS04330 (NCTC8067_00868)	-	894325..894822 (+)	498	WP_048378216.1	methylated-DNA--[protein]-cysteine S-methyltransferase	-
EL287_RS04335 (NCTC8067_00869)	nadC	894877..895725 (-)	849	WP_048378215.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 286 a.a.
• Molecular weight: 31365.50 Da
• Isoelectric Point: 7.9345

>NTDB_id=1122653 EL287_RS04310 WP_048378220.1 889956..890816(-) (pilD) [Pseudomonas taetrolens strain NCTC8067]
MIDFLSFHPGAFIACALVLGLLVGSFINVLVWRLPKMLEHDWQAQARELLAMPAAEKGPVYNLMLPHSHCPHCQHPLRAR
ENIPLLSYALLRGKCSQCNTAISLRYPLTELACGLLSAFIAWHFGVSGQAGWMLVLTWGLLAICLIDLEHQIVPDVLVLP
LLWLGLLLNSFDMFTSLSQAVWGAALGYASLWSIFWIFKLLTGKDGMGYGDFKLLAMLGAWGGIAILPLTLLLSSLVGAL
VGLSLLGLRRAKTSTPIPFAPYLAIAGWLALLWGGQITTFYWQLAV

Nucleotide

• Length: 861 bp

>NTDB_id=1122653 EL287_RS04310 WP_048378220.1 889956..890816(-) (pilD) [Pseudomonas taetrolens strain NCTC8067]
ATGATTGATTTTCTGAGTTTTCACCCCGGTGCCTTCATCGCTTGTGCTTTGGTACTGGGCTTGCTGGTGGGCAGTTTTAT
CAATGTGCTGGTGTGGCGTCTGCCCAAAATGCTCGAACATGACTGGCAAGCCCAGGCCCGGGAGCTGCTGGCAATGCCGG
CCGCTGAAAAAGGCCCTGTGTATAACTTGATGTTGCCCCACTCGCACTGCCCGCATTGCCAGCATCCCCTGCGCGCCCGG
GAAAATATTCCGTTACTGAGCTATGCCTTGCTGCGTGGCAAGTGCAGCCAGTGCAATACAGCGATCAGTCTGCGTTATCC
CCTGACCGAACTGGCCTGCGGGCTGCTGTCAGCCTTCATCGCCTGGCATTTCGGTGTTAGCGGGCAAGCGGGCTGGATGC
TGGTGCTGACGTGGGGATTGCTCGCCATCTGCCTGATTGACCTCGAGCACCAGATCGTACCCGACGTGCTGGTCTTGCCC
TTGCTTTGGCTGGGCTTGCTGCTGAACAGTTTCGATATGTTCACCAGCTTGTCGCAAGCCGTATGGGGAGCTGCGCTGGG
CTATGCCAGTCTGTGGTCAATCTTCTGGATATTCAAACTGCTCACCGGCAAGGACGGAATGGGCTACGGCGACTTCAAAT
TGCTGGCCATGCTCGGCGCCTGGGGAGGCATCGCCATTTTGCCTCTGACCCTCTTGCTCTCGTCGCTGGTGGGCGCCCTC
GTGGGGCTGAGCCTGCTGGGATTACGCCGCGCCAAAACATCCACTCCCATCCCCTTTGCGCCGTATCTGGCAATTGCCGG
TTGGCTTGCATTGCTCTGGGGTGGTCAAATAACCACTTTCTATTGGCAGCTTGCGGTTTGA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

• Type IV leader peptidase family (Peptidase_A24) (134-243)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	48.951	100	0.49
pilD	Acinetobacter baumannii D1279779	48.897	95.105	0.465
pilD	Acinetobacter nosocomialis M2	48.529	95.105	0.462
pilD	Vibrio campbellii strain DS40M4	45.263	99.65	0.451
pilD	Neisseria gonorrhoeae MS11	45.926	94.406	0.434