Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EL239_RS04640 Genome accession   NZ_LR134374
Coordinates   1077147..1079288 (-) Length   713 a.a.
NCBI ID   WP_058482982.1    Uniprot ID   A0A0W0Z6Y2
Organism   Legionella spiritensis strain NCTC12082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1072147..1084288
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL239_RS04615 (NCTC12082_00939) - 1072548..1072988 (-) 441 WP_126338533.1 universal stress protein -
  EL239_RS04620 pnuC 1073414..1074007 (+) 594 WP_126338535.1 nicotinamide riboside transporter PnuC -
  EL239_RS04625 (NCTC12082_00941) - 1073990..1075435 (-) 1446 WP_126338537.1 AAA family ATPase -
  EL239_RS04630 (NCTC12082_00942) aroB 1075439..1076545 (-) 1107 WP_126338539.1 3-dehydroquinate synthase -
  EL239_RS04635 (NCTC12082_00943) aroK 1076532..1077059 (-) 528 WP_058482981.1 shikimate kinase AroK -
  EL239_RS04640 (NCTC12082_00944) pilQ 1077147..1079288 (-) 2142 WP_058482982.1 type IV pilus secretin PilQ Machinery gene
  EL239_RS04645 (NCTC12082_00945) pilP 1079298..1079804 (-) 507 WP_058483009.1 pilus assembly protein PilP Machinery gene
  EL239_RS04650 (NCTC12082_00946) pilO 1079819..1080439 (-) 621 WP_126338541.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL239_RS04655 (NCTC12082_00947) pilN 1080436..1081014 (-) 579 WP_058482984.1 PilN domain-containing protein Machinery gene
  EL239_RS04660 (NCTC12082_00948) pilM 1081019..1082083 (-) 1065 WP_126338543.1 pilus assembly protein PilM Machinery gene
  EL239_RS04665 (NCTC12082_00949) - 1082428..1083546 (+) 1119 WP_126338545.1 hypothetical protein -

Sequence

Protein


Download         Length: 713 a.a.        Molecular weight: 78454.70 Da        Isoelectric Point: 10.1096

>NTDB_id=1122391 EL239_RS04640 WP_058482982.1 1077147..1079288(-) (pilQ) [Legionella spiritensis strain NCTC12082]
MRKIITSSLLLLVICFGHVFAKDNALVGVKVIPLPDNRIRVDFQFADAVKQLPASFITPKPPRIILDFIQSSNQLDPSLQ
SKKIEVGALRNYKIVAVKDRVRAILDLSDSVSYSGYLSNKIYSITLSGKSNQIFQNRKEVFISNRVPRTRYGINKIDFRG
TGKGGGRVIIDVSGSSIPIDVTQTGKEVVVNFTSTGVPLRLAKRYDVSDFQSPAELITLQQAGKNARLTIVNRGDYGHFA
YQVNKQFIVDVFPLTAEEIKRAKLKKQIFVGKRISLNFQNIKIRSVLQLLAEFTGINMVVSDQVQGDITLRLNDIPWDQA
LDIILKTNSLDKRKTGNIMLIAPAKELLNQEKAELKAQQEVTSLAPLRSDLLQINYAKAADIATLLKDKNNSLLSKRGNL
SVDTRTNTIWIQDTGLQIEEIRELVKKLDIPVKQVQIEARIVNVTKDFTRDLGIRFGVSKPTHLSGTLSGANQLAQNVLP
ADVTPFTDRLNLDLAAVPEGLAPASIGLAVARLGDGILLDLELSALESEGKGEVISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDDKILMDLQINQDTPSAQTFQGVPSILTKEIQTNVLVNNGQTIVLGGIYKQTKN
NEINRVPFLGELPVVGVLFRNQSETINNEELLIFITPRIITNTLSITTIEGRGKAVAKGVELDKFGKPVRPYK

Nucleotide


Download         Length: 2142 bp        

>NTDB_id=1122391 EL239_RS04640 WP_058482982.1 1077147..1079288(-) (pilQ) [Legionella spiritensis strain NCTC12082]
TTGCGTAAGATCATTACTTCATCATTACTCTTGCTGGTGATTTGCTTTGGCCACGTATTTGCCAAAGATAACGCATTGGT
CGGTGTTAAAGTCATTCCGTTACCCGATAACCGTATACGGGTTGATTTTCAGTTTGCGGACGCGGTCAAACAACTGCCGG
CAAGTTTTATCACTCCGAAACCACCCCGTATTATTCTTGATTTTATTCAAAGCTCCAACCAGCTTGATCCATCCCTGCAG
TCTAAAAAAATAGAGGTCGGAGCTCTTCGGAACTATAAAATCGTCGCGGTGAAGGATAGGGTCCGGGCCATTCTGGATTT
GTCCGATTCCGTTTCTTATTCGGGGTATTTGTCTAACAAAATTTATTCCATCACCCTGAGTGGCAAAAGTAATCAGATTT
TTCAAAACCGCAAGGAAGTATTCATCAGTAACCGGGTACCACGTACCAGATACGGTATTAACAAAATCGATTTCCGAGGC
ACAGGAAAGGGTGGTGGCCGGGTTATTATCGATGTTTCCGGCAGCAGCATACCAATTGATGTGACTCAGACCGGCAAGGA
AGTTGTCGTCAATTTCACCAGTACCGGTGTGCCGCTAAGGCTGGCGAAACGGTATGATGTCAGCGACTTTCAAAGTCCCG
CGGAATTAATCACCTTGCAGCAGGCGGGTAAAAACGCCCGCCTGACCATTGTTAACCGGGGCGACTACGGCCATTTTGCC
TATCAGGTTAATAAACAGTTTATTGTGGATGTTTTTCCGTTAACGGCCGAGGAAATCAAGCGGGCGAAGCTCAAAAAGCA
GATTTTTGTCGGCAAACGTATTTCCCTGAATTTCCAGAATATCAAGATACGTTCCGTGTTACAGTTGCTGGCGGAATTCA
CGGGCATTAATATGGTGGTCAGCGATCAGGTGCAGGGAGATATCACTTTGCGATTGAACGATATTCCCTGGGATCAGGCT
CTGGATATTATTTTGAAAACCAATTCTCTGGACAAGCGCAAAACAGGCAATATTATGTTGATTGCTCCGGCCAAGGAATT
GCTCAATCAGGAAAAGGCGGAATTAAAAGCGCAACAGGAAGTAACCAGTCTGGCGCCGTTGCGTTCCGATCTTTTGCAGA
TTAATTACGCGAAAGCGGCGGATATTGCGACTTTGCTTAAAGATAAAAATAATTCGCTTCTGTCCAAACGCGGTAATTTA
AGTGTTGATACCCGCACCAATACTATCTGGATACAGGATACGGGTTTGCAGATTGAGGAAATCAGAGAATTAGTCAAGAA
ACTGGATATACCGGTCAAACAGGTTCAGATTGAAGCACGTATCGTCAATGTGACGAAAGATTTTACCCGGGATTTGGGTA
TACGCTTCGGGGTCTCCAAACCCACTCATTTAAGTGGTACCTTAAGTGGTGCAAACCAGTTGGCGCAAAATGTTTTGCCG
GCGGATGTGACGCCATTTACCGATCGGTTGAACCTGGATTTGGCGGCGGTTCCCGAGGGACTGGCTCCTGCTTCCATCGG
TCTTGCGGTTGCCAGGCTGGGCGACGGTATTTTACTGGATTTGGAATTGTCCGCACTGGAAAGCGAGGGCAAGGGGGAGG
TTATTTCCAGTCCAAGGTTGATTACAACCAACCAGCAGGCAGCCGTGATTGAGTCCGGGGAAGAGATTCCCTATCAGGAA
GCCACATCCAGTGGTGCTACGGCAGTGGCTTTTAAAAAGGCGGTATTAAGTTTGAAAGTGACGCCCCAGATTACTCCGGA
CGACAAAATCCTGATGGACTTGCAAATCAATCAGGATACCCCGTCAGCCCAGACTTTTCAGGGCGTTCCCTCGATTTTAA
CCAAGGAAATCCAGACCAATGTCCTGGTTAATAATGGCCAGACGATTGTTTTGGGTGGTATTTACAAGCAAACGAAAAAC
AATGAAATTAACCGTGTTCCTTTTCTTGGCGAACTTCCGGTTGTTGGGGTACTATTCCGCAATCAATCCGAAACAATTAA
TAATGAGGAGTTGCTCATTTTCATTACTCCTAGGATAATAACCAACACCTTGTCTATAACTACTATTGAAGGACGGGGCA
AAGCTGTTGCTAAAGGAGTTGAATTGGATAAGTTCGGAAAGCCTGTAAGGCCCTATAAATAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(525-680)

Bacterial type II/III secretion system short domain (Secretin_N)

(372-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0Z6Y2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain ERS1305867

72.353

95.372

0.69
  pilQ Legionella pneumophila strain Lp02

72.353

95.372

0.69
  comQ Acinetobacter baylyi ADP1

39.504

96.213

0.38
  pilQ Pseudomonas aeruginosa PAK

37.872

98.878

0.374
  pilQ Acinetobacter baumannii strain A118

37.411

98.597

0.369
  pilQ Acinetobacter baumannii D1279779

37.411

98.597

0.369

Multiple sequence alignment