Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EL239_RS04650 Genome accession   NZ_LR134374
Coordinates   1079819..1080439 (-) Length   206 a.a.
NCBI ID   WP_126338541.1    Uniprot ID   -
Organism   Legionella spiritensis strain NCTC12082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1074819..1085439
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL239_RS04630 (NCTC12082_00942) aroB 1075439..1076545 (-) 1107 WP_126338539.1 3-dehydroquinate synthase -
  EL239_RS04635 (NCTC12082_00943) aroK 1076532..1077059 (-) 528 WP_058482981.1 shikimate kinase AroK -
  EL239_RS04640 (NCTC12082_00944) pilQ 1077147..1079288 (-) 2142 WP_058482982.1 type IV pilus secretin PilQ Machinery gene
  EL239_RS04645 (NCTC12082_00945) pilP 1079298..1079804 (-) 507 WP_058483009.1 pilus assembly protein PilP Machinery gene
  EL239_RS04650 (NCTC12082_00946) pilO 1079819..1080439 (-) 621 WP_126338541.1 type 4a pilus biogenesis protein PilO Machinery gene
  EL239_RS04655 (NCTC12082_00947) pilN 1080436..1081014 (-) 579 WP_058482984.1 PilN domain-containing protein Machinery gene
  EL239_RS04660 (NCTC12082_00948) pilM 1081019..1082083 (-) 1065 WP_126338543.1 pilus assembly protein PilM Machinery gene
  EL239_RS04665 (NCTC12082_00949) - 1082428..1083546 (+) 1119 WP_126338545.1 hypothetical protein -

Sequence

Protein


Download         Length: 206 a.a.        Molecular weight: 23559.32 Da        Isoelectric Point: 6.2543

>NTDB_id=1122393 EL239_RS04650 WP_126338541.1 1079819..1080439(-) (pilO) [Legionella spiritensis strain NCTC12082]
MNNINLNELTLDNIGQWPAPVKYLLAAIVAILIVALGYWVIVKPNIEQYDALLVQEETLRAEFEQKQQMASNLQAYRVQL
QIIKERFGNMLRQLPAQNEMPGLVEDISKTGIASGLTFELFAPQPEVAHDFYIELPINITVVGNYHQLALFLSRIAQMSR
IVTLHDFEISSASEDESQTRGDKKIKNKPDLLRMKITAKIYRYRTL

Nucleotide


Download         Length: 621 bp        

>NTDB_id=1122393 EL239_RS04650 WP_126338541.1 1079819..1080439(-) (pilO) [Legionella spiritensis strain NCTC12082]
ATGAATAACATCAATCTCAATGAGTTGACATTGGATAATATCGGACAATGGCCCGCGCCAGTGAAGTATCTGTTGGCCGC
TATTGTTGCCATATTGATTGTCGCATTGGGTTACTGGGTGATTGTGAAGCCAAATATTGAACAGTATGATGCCCTGTTGG
TACAAGAGGAAACATTAAGAGCCGAGTTTGAGCAAAAACAGCAAATGGCATCCAATCTTCAGGCCTATCGGGTGCAGCTA
CAGATTATCAAAGAACGTTTTGGAAACATGCTCAGACAGCTCCCGGCTCAAAACGAAATGCCCGGTCTGGTGGAGGATAT
CTCAAAAACCGGGATCGCAAGTGGTCTGACTTTTGAATTATTTGCGCCTCAGCCTGAGGTGGCGCATGATTTTTATATCG
AGTTACCGATCAACATAACGGTAGTAGGCAATTATCATCAACTGGCTTTATTTTTAAGCAGAATTGCTCAAATGAGCCGG
ATTGTGACGCTGCACGATTTCGAAATCAGCAGTGCTTCCGAGGATGAATCGCAGACTCGGGGGGATAAAAAAATAAAAAA
TAAACCCGATTTGCTAAGAATGAAAATTACCGCGAAAATTTATCGGTATCGTACGCTATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(55-204)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

63.235

99.029

0.626
  comO Acinetobacter baylyi ADP1

40

97.087

0.388
  pilO Acinetobacter baumannii D1279779

39.303

97.573

0.383

Multiple sequence alignment