Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EL239_RS02955 Genome accession   NZ_LR134374
Coordinates   669924..671150 (+) Length   408 a.a.
NCBI ID   WP_126338045.1    Uniprot ID   -
Organism   Legionella spiritensis strain NCTC12082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 664924..676150
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL239_RS02935 (NCTC12082_00595) - 666131..666694 (-) 564 WP_058484329.1 hypoxanthine-guanine phosphoribosyltransferase -
  EL239_RS02940 (NCTC12082_00596) - 666788..667120 (-) 333 WP_126338040.1 DUF1820 family protein -
  EL239_RS02945 (NCTC12082_00597) - 667163..667930 (-) 768 WP_172597030.1 class I SAM-dependent methyltransferase -
  EL239_RS02950 (NCTC12082_00598) pilB 668193..669917 (+) 1725 WP_126338043.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL239_RS02955 (NCTC12082_00599) pilC 669924..671150 (+) 1227 WP_126338045.1 type II secretion system F family protein Machinery gene
  EL239_RS02960 (NCTC12082_00600) - 671204..672067 (+) 864 WP_232001725.1 prepilin peptidase -
  EL239_RS02965 (NCTC12082_00601) - 672082..672672 (-) 591 WP_058484323.1 sel1 repeat family protein -
  EL239_RS02970 (NCTC12082_00602) - 672684..673136 (-) 453 WP_126338050.1 hypothetical protein -
  EL239_RS02975 (NCTC12082_00603) - 673304..674119 (+) 816 WP_058484321.1 pyruvate, water dikinase regulatory protein -
  EL239_RS02980 (NCTC12082_00604) - 674293..675738 (-) 1446 WP_126338051.1 bifunctional 2-methylcitrate dehydratase/aconitate hydratase -

Sequence

Protein


Download         Length: 408 a.a.        Molecular weight: 44832.59 Da        Isoelectric Point: 9.3870

>NTDB_id=1122387 EL239_RS02955 WP_126338045.1 669924..671150(+) (pilC) [Legionella spiritensis strain NCTC12082]
MDKSKNANAIISYQWEGVNKTGEKVNGIIDARSLALAKADLRKQGIITKKVIKKRKPLFDKKNRKITQGDITVFSRQMAT
MIEAGIPLVQSFDIVSKGQSNNRMRDLIEGVKKDVETGLTLAEALQKHPEYFNDLFCNLVDAGEKSGSLEIMLDKVATYK
EKIETIKKKIKKALTYPMAVLVVAFIVTAALLIFVVPQFQSLFEGFGADLPAMTKAVVELSHFFQAYWYLIFGVLGGLIY
SFVYAKNHSPNFAQTIDRSMLKFPVIGPILEKAAIARFSRTLSITFAAGLPLVDALKSVSGATGNILYAKATDKIREDVS
TGQQMQVAMDNTHLFPNMVIQMVAIGEESGALEQMLGKVADFYEEEVDNAVDSLSSLLEPIIMSILGILVGGLVVAMYLP
IFKLGSAV

Nucleotide


Download         Length: 1227 bp        

>NTDB_id=1122387 EL239_RS02955 WP_126338045.1 669924..671150(+) (pilC) [Legionella spiritensis strain NCTC12082]
ATGGATAAAAGTAAAAATGCCAACGCCATCATCAGCTACCAATGGGAGGGCGTCAACAAAACAGGTGAAAAAGTCAATGG
CATCATTGATGCCAGAAGCCTGGCATTGGCAAAAGCGGATTTGCGAAAACAAGGCATCATCACTAAAAAGGTGATTAAAA
AAAGAAAACCCCTATTTGACAAAAAAAACAGGAAAATAACCCAGGGCGATATCACTGTTTTCAGCCGACAGATGGCCACG
ATGATCGAGGCGGGTATTCCCCTGGTACAATCGTTCGATATTGTTTCCAAGGGACAGAGTAACAACCGTATGCGCGACTT
GATTGAAGGCGTAAAAAAAGATGTGGAAACAGGGCTGACTTTAGCGGAAGCGTTACAAAAACACCCCGAATATTTTAATG
ATTTGTTTTGTAATCTGGTCGATGCCGGTGAAAAATCAGGTTCTCTGGAAATTATGCTTGATAAGGTCGCCACCTATAAG
GAAAAAATTGAAACCATCAAGAAAAAAATTAAAAAAGCACTTACTTATCCCATGGCTGTATTGGTTGTGGCATTCATCGT
AACAGCCGCATTACTAATCTTTGTGGTACCACAATTTCAATCGTTATTTGAAGGATTCGGAGCGGATCTTCCGGCCATGA
CCAAAGCGGTTGTGGAATTGTCTCACTTTTTCCAGGCGTACTGGTATCTTATATTTGGCGTGTTGGGCGGCCTCATTTAT
TCATTTGTCTATGCCAAAAATCACTCGCCTAATTTTGCGCAAACGATTGACCGTTCGATGCTTAAATTTCCGGTTATTGG
ACCTATTCTTGAAAAAGCGGCTATCGCACGCTTTTCCCGTACCTTATCCATTACCTTTGCGGCAGGTTTGCCTCTGGTTG
ACGCCCTGAAATCGGTATCCGGCGCTACAGGTAACATTCTCTACGCCAAGGCCACTGATAAAATCAGGGAGGATGTATCT
ACAGGCCAGCAAATGCAGGTTGCTATGGACAACACTCATTTGTTTCCCAATATGGTCATTCAAATGGTCGCTATCGGCGA
GGAGTCCGGAGCCCTGGAACAAATGCTTGGTAAAGTAGCTGATTTTTATGAGGAAGAAGTGGATAATGCGGTTGACTCTT
TAAGTAGTTTGCTGGAACCTATTATCATGTCAATTTTGGGTATCCTGGTCGGGGGATTGGTAGTTGCAATGTATCTGCCT
ATTTTCAAACTGGGTTCCGCCGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(74-197)

Type II secretion system (T2SS), protein F (T2SSF)

(278-400)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

80.941

99.02

0.801
  pilC Pseudomonas stutzeri DSM 10701

56.818

97.059

0.551
  pilC Acinetobacter baylyi ADP1

54.68

99.51

0.544
  pilC Acinetobacter baumannii D1279779

54.568

99.265

0.542
  pilG Neisseria meningitidis 44/76-A

46.096

97.304

0.449
  pilG Neisseria gonorrhoeae MS11

45.844

97.304

0.446
  pilC Vibrio cholerae strain A1552

42.317

97.304

0.412
  pilC Vibrio campbellii strain DS40M4

41.562

97.304

0.404
  pilC Thermus thermophilus HB27

35.749

100

0.363

Multiple sequence alignment