Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL239_RS02950 Genome accession   NZ_LR134374
Coordinates   668193..669917 (+) Length   574 a.a.
NCBI ID   WP_126338043.1    Uniprot ID   -
Organism   Legionella spiritensis strain NCTC12082     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 663193..674917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL239_RS02930 (NCTC12082_00593) - 664630..665763 (+) 1134 WP_126338038.1 HlyD family type I secretion periplasmic adaptor subunit -
  EL239_RS02935 (NCTC12082_00595) - 666131..666694 (-) 564 WP_058484329.1 hypoxanthine-guanine phosphoribosyltransferase -
  EL239_RS02940 (NCTC12082_00596) - 666788..667120 (-) 333 WP_126338040.1 DUF1820 family protein -
  EL239_RS02945 (NCTC12082_00597) - 667163..667930 (-) 768 WP_172597030.1 class I SAM-dependent methyltransferase -
  EL239_RS02950 (NCTC12082_00598) pilB 668193..669917 (+) 1725 WP_126338043.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL239_RS02955 (NCTC12082_00599) pilC 669924..671150 (+) 1227 WP_126338045.1 type II secretion system F family protein Machinery gene
  EL239_RS02960 (NCTC12082_00600) - 671204..672067 (+) 864 WP_232001725.1 prepilin peptidase -
  EL239_RS02965 (NCTC12082_00601) - 672082..672672 (-) 591 WP_058484323.1 sel1 repeat family protein -
  EL239_RS02970 (NCTC12082_00602) - 672684..673136 (-) 453 WP_126338050.1 hypothetical protein -
  EL239_RS02975 (NCTC12082_00603) - 673304..674119 (+) 816 WP_058484321.1 pyruvate, water dikinase regulatory protein -

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 63212.33 Da        Isoelectric Point: 5.3257

>NTDB_id=1122386 EL239_RS02950 WP_126338043.1 668193..669917(+) (pilB) [Legionella spiritensis strain NCTC12082]
MATAAEEQRLQGIAQLMVHENLLDKEMAMEYQNQAQANKQSLLQYLVANEVLSAEKIALSVANNFGVPLIDLDCIEPDTL
PTTLVNEKLIRRHAIIPLFTRGNHLFLATDDPSKQASLKEIQFHTGLHSNAIVVETNKLSKLIDKLLNEKESQGLTDYVD
DSSDLEGLEISAEDEEQDMDASSSANDDAPVVKFVNKILVEAIKNGVSDIHFEPYEKEYRIRYRQDGILTEVATPPVSLS
SRITSRIKIMSNLDISERRIPQDGRFKMKLSRTRAIDFRVSTCPTVGGEKVVMRILDPGSTKLGIEALGFNPDQRKHFLH
AIEKPQGMILVTGPTGSGKTVTLYTALNILNTKEANISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSASETLTRLLNMGVPSFNIASSVTLLIAQRLARRLCESCKAPRDDFTIES
LMELGYSNEDAKSLKPYKAVGCSQCTNGYRGRIGLFEVLPMSKAIGELIMKGGNSLDILKQAQSEGMLTIHESGLKKIQE
GMTTIEEVNRVTVD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=1122386 EL239_RS02950 WP_126338043.1 668193..669917(+) (pilB) [Legionella spiritensis strain NCTC12082]
ATGGCTACTGCTGCAGAAGAACAACGTTTACAAGGTATTGCCCAGCTCATGGTCCACGAGAATCTCCTGGATAAGGAGAT
GGCTATGGAATACCAGAATCAGGCTCAAGCCAATAAACAGAGTTTGCTGCAATATCTTGTGGCAAACGAAGTCTTGTCCG
CTGAAAAAATTGCGCTCAGTGTCGCAAATAATTTTGGTGTACCGCTCATCGACCTGGATTGCATAGAACCGGATACTTTG
CCAACCACCCTCGTCAATGAAAAATTAATACGGCGTCATGCCATTATTCCCTTATTCACCCGGGGCAATCATCTTTTTCT
TGCTACCGACGATCCTAGCAAACAAGCCTCGTTAAAGGAAATCCAGTTTCATACGGGTCTTCATTCCAATGCGATCGTTG
TAGAGACGAACAAACTAAGCAAACTGATAGACAAACTGCTGAATGAAAAGGAAAGTCAGGGTTTAACCGATTATGTGGAT
GATTCCAGTGATCTTGAAGGACTTGAAATCAGTGCGGAAGATGAAGAACAGGACATGGATGCCTCATCTTCGGCCAACGA
CGATGCACCCGTCGTAAAATTCGTCAACAAGATTCTGGTGGAAGCCATTAAAAACGGCGTTTCAGATATTCATTTTGAGC
CTTATGAGAAAGAATATCGTATACGATACCGTCAAGACGGCATTCTTACGGAAGTCGCCACCCCTCCCGTAAGTCTGTCC
AGCCGGATTACATCACGCATCAAAATCATGTCAAATCTTGATATTTCCGAACGACGCATCCCTCAGGACGGGCGCTTTAA
AATGAAGTTATCGCGAACCAGAGCCATAGACTTTCGCGTTAGTACCTGCCCTACTGTCGGTGGAGAAAAAGTAGTAATGC
GTATACTGGATCCGGGTTCAACCAAACTCGGAATCGAAGCGTTAGGATTTAATCCTGATCAAAGGAAACACTTCCTGCAC
GCCATAGAAAAACCCCAGGGAATGATTTTAGTAACCGGCCCTACAGGAAGCGGAAAAACCGTAACGCTTTACACGGCACT
CAATATCCTGAATACCAAGGAGGCCAACATCTCTACGGCGGAGGATCCGGTAGAGATAAAAGTTCCCGGAATTAATCAAG
TGAATATTAACCCTAAAGCGGGATTAACATTTTCCGGTGCACTTCGATCATTTTTACGCCAGGATCCGGACATTATCATG
GTCGGGGAAATTCGTGATCTTGAGACGGCAGAAATCGCTGTTAAAGCGGCACAGACCGGACATTTGGTTCTGTCAACACT
ACACACCAACAGCGCGTCTGAAACCCTGACCCGCCTACTGAATATGGGCGTTCCTTCCTTTAACATTGCCAGCTCGGTCA
CTCTGCTCATAGCACAACGACTGGCAAGGCGATTATGCGAAAGTTGCAAGGCACCACGTGATGATTTCACCATCGAAAGC
CTGATGGAGCTAGGGTACAGCAATGAAGATGCAAAATCCTTAAAACCCTATAAAGCGGTTGGATGCAGCCAGTGCACTAA
CGGGTATCGAGGACGTATCGGTTTGTTTGAAGTTCTCCCCATGTCGAAAGCCATAGGGGAATTGATCATGAAAGGCGGCA
ACTCTCTGGACATTCTTAAGCAAGCCCAATCCGAGGGGATGTTAACCATACACGAATCCGGATTAAAAAAAATTCAGGAA
GGCATGACCACTATTGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)

Type II/IV secretion system protein (T2SSE)

(197-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

77.352

100

0.774
  pilB Acinetobacter baumannii D1279779

51.494

99.129

0.51
  pilB Acinetobacter baylyi ADP1

49.74

100

0.5
  pilF Neisseria gonorrhoeae MS11

47.266

98.78

0.467
  pilB Vibrio cholerae strain A1552

49.168

94.251

0.463
  pilB Vibrio campbellii strain DS40M4

46.29

98.606

0.456
  pilB Vibrio parahaemolyticus RIMD 2210633

47.874

94.251

0.451
  pilF Thermus thermophilus HB27

38.676

100

0.387
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.698

92.16

0.366
  pilB/pilB1 Synechocystis sp. PCC 6803

38

95.819

0.364

Multiple sequence alignment