Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilG   Type   Machinery gene
Locus tag   EL143_RS04875 Genome accession   NZ_LR134313
Coordinates   1057527..1058774 (+) Length   415 a.a.
NCBI ID   WP_085416670.1    Uniprot ID   A0A1X3CXU9
Organism   Neisseria canis strain NCTC10296     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1052527..1063774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL143_RS04855 (NCTC10296_00974) - 1052815..1053792 (+) 978 Protein_970 IS30 family transposase -
  EL143_RS04860 (NCTC10296_00975) - 1053854..1054324 (-) 471 WP_085416673.1 low molecular weight protein-tyrosine-phosphatase -
  EL143_RS04865 (NCTC10296_00976) - 1054350..1055447 (-) 1098 WP_085416672.1 conjugal transfer protein -
  EL143_RS04870 (NCTC10296_00977) pilF 1055809..1057485 (+) 1677 WP_085416671.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL143_RS04875 (NCTC10296_00978) pilG 1057527..1058774 (+) 1248 WP_085416670.1 type II secretion system F family protein Machinery gene
  EL143_RS04880 (NCTC10296_00979) pilD 1058761..1059639 (+) 879 WP_126326629.1 A24 family peptidase Machinery gene
  EL143_RS04885 (NCTC10296_00980) coaE 1059636..1060241 (+) 606 WP_085416669.1 dephospho-CoA kinase -
  EL143_RS04890 (NCTC10296_00981) yacG 1060420..1060602 (+) 183 WP_085416668.1 DNA gyrase inhibitor YacG -
  EL143_RS04895 (NCTC10296_00982) - 1060834..1061409 (+) 576 WP_085416667.1 hypothetical protein -
  EL143_RS04900 (NCTC10296_00983) - 1061585..1062976 (+) 1392 WP_197719619.1 chloride channel protein -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 45572.67 Da        Isoelectric Point: 10.0471

>NTDB_id=1121091 EL143_RS04875 WP_085416670.1 1057527..1058774(+) (pilG) [Neisseria canis strain NCTC10296]
MASNQNTGGLFGIGTKDKGRRFTFEGKNTLTDQIVRGEVVAKDEEEARKKLQRRSIKPLRISKVKSTRKKKITQADITVF
TRQLATMMKAGLPVMQAFEIVARGHSNPSMTQLLMQVRGDVEQGSPMSKAFAKHPKYFDRFYCNLIAAGETGGVLESLLD
KLALYKEKTQAIKKKVKSALTYPIAIVVVAIALVMVMMMFVLPEFGKVYEGMGAELPGLTQVMMTISRFFVEWGWLIIIL
MIASVIAAVKAVQNSPSLQRRVDAWLLRMPIFGNIVRKATIARWARTTSSLFAAGVPLVEALDSVAGASGNILYEEATQD
IRAKVNQGMSLTSSMQSTELFPNMVLQMASIGEEAGSLEDMLNKAAEFYEDEVDNAVAQLSSLMEPIIMVVLGSIIGVIL
VAMYLPLFNLGNVVG

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=1121091 EL143_RS04875 WP_085416670.1 1057527..1058774(+) (pilG) [Neisseria canis strain NCTC10296]
ATGGCATCAAATCAAAATACGGGTGGTCTTTTCGGTATTGGTACAAAAGACAAAGGTAGACGTTTTACGTTTGAGGGCAA
AAATACGCTGACCGACCAAATCGTTCGCGGCGAAGTGGTTGCCAAAGACGAGGAAGAAGCGCGTAAGAAGCTACAGCGGC
GCAGCATTAAACCTTTGCGGATCAGTAAAGTAAAGAGCACCCGTAAGAAGAAAATCACACAAGCCGACATCACGGTGTTT
ACCCGTCAACTGGCAACTATGATGAAAGCAGGCCTGCCTGTAATGCAGGCATTTGAGATCGTTGCCCGAGGCCACTCAAA
TCCTTCGATGACGCAATTGCTGATGCAGGTTCGCGGCGATGTGGAACAAGGTAGCCCGATGTCGAAGGCTTTTGCTAAAC
ATCCTAAATATTTCGACCGTTTTTACTGCAATTTGATCGCAGCCGGTGAAACAGGCGGTGTATTGGAAAGCCTGTTGGAT
AAGTTGGCATTGTATAAAGAAAAAACCCAGGCCATTAAAAAGAAAGTGAAAAGTGCCTTAACTTACCCGATTGCCATCGT
GGTGGTTGCTATAGCCCTGGTAATGGTGATGATGATGTTTGTATTGCCAGAATTCGGTAAAGTATACGAGGGGATGGGCG
CCGAATTGCCGGGTCTGACTCAAGTCATGATGACAATTTCCAGATTCTTTGTTGAGTGGGGTTGGTTGATTATTATCTTG
ATGATTGCTTCCGTGATTGCTGCTGTTAAAGCAGTGCAGAATTCACCCAGCTTGCAACGCCGTGTAGATGCTTGGCTGTT
GCGCATGCCTATTTTCGGTAATATTGTAAGAAAAGCAACCATTGCGCGGTGGGCGCGGACGACATCCAGCTTGTTTGCCG
CGGGCGTACCTTTGGTTGAAGCGCTGGATTCGGTGGCGGGTGCTTCGGGCAATATTTTATATGAAGAAGCAACACAGGAT
ATCCGTGCGAAAGTAAATCAGGGTATGTCGTTAACTTCAAGTATGCAAAGCACCGAGCTTTTCCCGAATATGGTGTTGCA
AATGGCTTCCATTGGTGAAGAAGCAGGTTCGTTGGAAGACATGTTGAACAAAGCGGCAGAATTTTATGAAGATGAAGTAG
ATAATGCTGTTGCACAATTGTCTTCATTAATGGAACCCATCATTATGGTGGTTTTAGGCTCCATTATCGGTGTGATCTTG
GTTGCCATGTATCTGCCCTTGTTCAATCTGGGTAATGTTGTGGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(284-406)

Type II secretion system (T2SS), protein F (T2SSF)

(80-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X3CXU9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilG Neisseria gonorrhoeae MS11

67.874

99.759

0.677
  pilG Neisseria meningitidis 44/76-A

67.391

99.759

0.672
  pilC Legionella pneumophila strain ERS1305867

46.734

95.904

0.448
  pilC Pseudomonas stutzeri DSM 10701

43.641

96.627

0.422
  pilC Acinetobacter baumannii D1279779

43.719

95.904

0.419
  pilC Acinetobacter baylyi ADP1

43.108

96.145

0.414
  pilC Vibrio cholerae strain A1552

38.903

96.627

0.376
  pilC Vibrio campbellii strain DS40M4

39

96.386

0.376

Multiple sequence alignment