Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   EL143_RS04870 Genome accession   NZ_LR134313
Coordinates   1055809..1057485 (+) Length   558 a.a.
NCBI ID   WP_085416671.1    Uniprot ID   A0A448D7F3
Organism   Neisseria canis strain NCTC10296     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1050809..1062485
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL143_RS04855 (NCTC10296_00974) - 1052815..1053792 (+) 978 Protein_970 IS30 family transposase -
  EL143_RS04860 (NCTC10296_00975) - 1053854..1054324 (-) 471 WP_085416673.1 low molecular weight protein-tyrosine-phosphatase -
  EL143_RS04865 (NCTC10296_00976) - 1054350..1055447 (-) 1098 WP_085416672.1 conjugal transfer protein -
  EL143_RS04870 (NCTC10296_00977) pilF 1055809..1057485 (+) 1677 WP_085416671.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL143_RS04875 (NCTC10296_00978) pilG 1057527..1058774 (+) 1248 WP_085416670.1 type II secretion system F family protein Machinery gene
  EL143_RS04880 (NCTC10296_00979) pilD 1058761..1059639 (+) 879 WP_126326629.1 A24 family peptidase Machinery gene
  EL143_RS04885 (NCTC10296_00980) coaE 1059636..1060241 (+) 606 WP_085416669.1 dephospho-CoA kinase -
  EL143_RS04890 (NCTC10296_00981) yacG 1060420..1060602 (+) 183 WP_085416668.1 DNA gyrase inhibitor YacG -
  EL143_RS04895 (NCTC10296_00982) - 1060834..1061409 (+) 576 WP_085416667.1 hypothetical protein -

Sequence

Protein


Download         Length: 558 a.a.        Molecular weight: 61870.26 Da        Isoelectric Point: 5.5684

>NTDB_id=1121090 EL143_RS04870 WP_085416671.1 1055809..1057485(+) (pilF) [Neisseria canis strain NCTC10296]
MSVGLLRILVQSQVISNEQAAGFQEALKHNKDIAPMLFEANIITPEGLAGLLAKLFNYPLLDLSYYPRNNVLTDVLSEEA
MVQNRCLPIFRRGNKVFLAVADPTQIQTYQKIAFTAGVTFDLVVVRSDQLDSILEWQNQRSTSILKEMSLEQDIAASSQA
LYIDNEEAEDGPIPRFIHKTLSDALSVGASDIHFEFYEQVARVRFRVDGQLREVVQPPLAVRGQLASRIKVMARLDISEK
RVPQDGRIQIAFSKHGRPIDFRVNTLPTLFGEKVVMRILNSDASTLNIDQLGFEPFQKELLLEAIHRPYGMVLVTGPTGS
GKTVSLYTCLNILNTENVNISTAEDPAEINLPGINQVNVNEKQGLTFASALKAFLRQDPDIIMVGEIRDLETADIAIKAS
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCSCKAPVERPPVEALKHAGFTDEDLAKDWTLY
RPVGCDRCRGKGFKGRAGVYEVMPITEEMQRVIMEGGNEVDILAMAYKEGMVDLRRAGLLKVMQGITSLEEVLASTND

Nucleotide


Download         Length: 1677 bp        

>NTDB_id=1121090 EL143_RS04870 WP_085416671.1 1055809..1057485(+) (pilF) [Neisseria canis strain NCTC10296]
ATGAGTGTAGGCTTACTTCGTATTCTGGTGCAAAGCCAGGTTATTTCTAATGAACAAGCAGCGGGATTTCAAGAAGCGCT
GAAACACAACAAAGATATTGCGCCCATGCTGTTCGAGGCCAACATTATTACGCCGGAAGGTCTGGCGGGCTTGTTGGCGA
AACTGTTCAATTATCCTTTACTGGATTTGTCTTATTATCCCAGAAACAACGTGCTGACCGATGTTTTGTCGGAAGAAGCA
ATGGTGCAAAACCGCTGCCTGCCGATTTTCAGGAGGGGCAATAAGGTATTTCTGGCGGTTGCCGATCCCACGCAAATTCA
AACTTATCAAAAAATCGCATTTACAGCCGGCGTAACTTTCGACTTGGTTGTGGTGCGCAGCGACCAGCTGGATTCAATAC
TGGAATGGCAAAACCAGCGCTCCACTTCGATTCTGAAAGAAATGAGCCTGGAGCAGGATATAGCTGCTTCCTCTCAGGCT
CTTTATATTGATAACGAAGAAGCCGAAGACGGCCCGATTCCGCGCTTTATCCATAAAACTCTGTCAGATGCACTCAGCGT
CGGCGCATCCGATATTCACTTTGAATTTTACGAGCAAGTCGCGCGCGTACGTTTCCGTGTGGACGGCCAATTGAGGGAAG
TGGTACAGCCGCCTTTGGCTGTACGCGGCCAACTGGCTTCGCGTATTAAGGTAATGGCGCGCTTGGATATTTCGGAAAAA
CGCGTTCCCCAAGACGGCCGTATCCAAATCGCTTTCTCCAAACACGGCCGCCCGATTGACTTCCGTGTAAATACACTGCC
TACTTTGTTTGGTGAAAAAGTGGTAATGCGTATTCTGAACTCGGATGCCAGTACCCTTAATATCGACCAATTGGGTTTTG
AGCCTTTCCAAAAAGAATTGTTGCTGGAAGCTATTCACCGTCCTTACGGCATGGTGCTGGTAACGGGGCCTACGGGTTCG
GGTAAAACGGTTTCGCTTTATACCTGTTTGAATATTCTGAATACCGAAAATGTTAATATTTCCACTGCAGAAGATCCGGC
AGAGATTAACTTACCCGGCATCAATCAGGTAAACGTTAATGAAAAGCAAGGCCTTACGTTCGCTTCTGCATTGAAAGCAT
TTTTGCGGCAGGATCCGGACATCATCATGGTGGGTGAGATTCGCGATTTGGAAACAGCGGATATTGCGATTAAAGCTTCT
CAAACCGGCCATATGGTTTTTTCAACGCTGCACACCAATAACGCTCCGGCAACGCTTTCCCGTTTGCTTAATATGGGCGT
GGCGCCGTTCAATATTGCTAGTTCTGTAAGCCTGATTATGGCTCAACGCCTATTGCGCCGTTTGTGCAGCTGCAAAGCAC
CAGTGGAACGCCCGCCTGTTGAAGCATTGAAACACGCCGGCTTTACGGACGAAGATTTAGCGAAGGATTGGACGCTTTAC
CGCCCCGTAGGTTGTGACCGCTGCCGCGGAAAAGGCTTTAAAGGCCGTGCCGGTGTTTATGAGGTTATGCCGATTACCGA
GGAAATGCAGCGTGTGATTATGGAAGGCGGTAATGAGGTTGATATTCTAGCAATGGCCTATAAAGAAGGCATGGTTGATT
TGCGCCGTGCCGGTTTGTTGAAAGTTATGCAAGGCATCACTTCTTTAGAAGAAGTGTTGGCGAGCACAAATGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(179-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(37-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A448D7F3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

76.386

100

0.765
  pilB Acinetobacter baumannii D1279779

52.236

100

0.523
  pilB Acinetobacter baylyi ADP1

52.065

99.821

0.52
  pilB Legionella pneumophila strain ERS1305867

48.036

100

0.482
  pilB Vibrio cholerae strain A1552

46.454

100

0.47
  pilB Vibrio parahaemolyticus RIMD 2210633

45.423

100

0.462
  pilB Vibrio campbellii strain DS40M4

43.339

100

0.437
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.579

93.728

0.371
  pilF Thermus thermophilus HB27

38.447

94.624

0.364

Multiple sequence alignment