Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   EL022_RS10830 Genome accession   NZ_LR134173
Coordinates   2557100..2558320 (-) Length   406 a.a.
NCBI ID   WP_028380563.1    Uniprot ID   A0A0W0S825
Organism   Legionella cherrii strain NCTC11976     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2552100..2563320
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL022_RS10805 (NCTC11976_02250) - 2552772..2553326 (-) 555 WP_028380568.1 HPF/RaiA family ribosome-associated protein -
  EL022_RS10810 (NCTC11976_02251) - 2553370..2553819 (-) 450 WP_028380567.1 CBS domain-containing protein -
  EL022_RS10815 (NCTC11976_02252) - 2554016..2555218 (-) 1203 WP_028380566.1 sterol desaturase family protein -
  EL022_RS10820 (NCTC11976_02253) - 2555234..2556004 (-) 771 WP_028380565.1 HAD-IA family hydrolase -
  EL022_RS10825 (NCTC11976_02254) - 2556115..2556975 (-) 861 WP_028380564.1 A24 family peptidase -
  EL022_RS10830 (NCTC11976_02255) pilC 2557100..2558320 (-) 1221 WP_028380563.1 type II secretion system F family protein Machinery gene
  EL022_RS10835 (NCTC11976_02256) pilB 2558332..2560053 (-) 1722 WP_028380562.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL022_RS10840 (NCTC11976_02257) - 2560360..2561142 (+) 783 WP_028380561.1 methyltransferase domain-containing protein -
  EL022_RS10845 (NCTC11976_02258) - 2561243..2561824 (-) 582 WP_028380560.1 hypothetical protein -
  EL022_RS10850 (NCTC11976_02259) - 2561979..2562305 (+) 327 WP_028380559.1 DUF1820 family protein -
  EL022_RS10855 (NCTC11976_02260) - 2562352..2562912 (+) 561 WP_028380558.1 hypoxanthine-guanine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44392.34 Da        Isoelectric Point: 9.8264

>NTDB_id=1118777 EL022_RS10830 WP_028380563.1 2557100..2558320(-) (pilC) [Legionella cherrii strain NCTC11976]
MKKNANTTLTFHYTGVNKAAQKINGDIDARSLALAKVELRRQGIIVSKIAKKRAPLFNFKSKKIKSGDITVFSRQLATMI
ESGIPLVQSFDIVAKGQTNKRLKELIENIKLDVETGLTLSEALMKHPAYFNELFCNLVEAGEKSGSLDIMLDKVATYKEK
IETIKKKIKKALTYPMAVMVVALIVTTGLLIFVVPQFESLFKGFGADLPAMTQAVVSMSKFFQSYWYLIFGALGGGVYAF
IYAEKHSPQFAQNVDRTLLKLPVIGPIIEKAAIARFSRTLSITFAAGLPLVEALKSVAGATGNIIFAKATDQIREEVSTG
QQMNKAMENTQLFPNMVIQMVAIGEESGALEKMLSKVADFYEEDVDNAVDSLSSLLEPVIMVVLGVLVGGLVVAMYLPIF
KLGSAI

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=1118777 EL022_RS10830 WP_028380563.1 2557100..2558320(-) (pilC) [Legionella cherrii strain NCTC11976]
ATGAAAAAAAATGCCAATACCACCTTAACTTTTCATTACACAGGTGTTAATAAAGCGGCTCAAAAAATAAATGGCGATAT
CGACGCCAGAAGTCTTGCTTTAGCTAAAGTAGAATTACGCAGACAGGGAATTATCGTAAGCAAAATTGCTAAAAAACGCG
CCCCTCTCTTCAATTTTAAAAGCAAGAAAATTAAGTCAGGTGATATCACGGTATTTAGTCGCCAACTCGCTACAATGATA
GAATCCGGGATACCGTTAGTGCAATCCTTTGATATTGTTGCAAAAGGACAGACAAATAAACGACTTAAAGAATTAATTGA
AAACATTAAACTTGATGTTGAAACAGGGTTAACTCTCTCAGAAGCATTGATGAAACATCCTGCCTATTTTAACGAATTAT
TTTGCAATTTGGTGGAAGCTGGAGAAAAGTCAGGTTCTCTAGATATCATGCTCGATAAAGTCGCCACATACAAAGAAAAA
ATAGAAACCATAAAAAAGAAAATTAAAAAGGCGTTAACTTACCCTATGGCCGTCATGGTTGTCGCTCTGATTGTTACGAC
CGGCTTACTTATTTTTGTTGTTCCTCAGTTTGAATCCCTATTTAAAGGTTTTGGTGCTGATCTACCTGCTATGACCCAAG
CAGTAGTGAGCATGTCCAAATTCTTTCAATCCTATTGGTATTTAATTTTTGGCGCATTGGGCGGAGGGGTTTACGCTTTT
ATTTATGCAGAAAAACACTCCCCTCAATTTGCACAAAATGTGGATAGAACCTTATTAAAGCTACCTGTTATTGGGCCTAT
TATTGAGAAAGCTGCAATCGCTCGATTTTCAAGAACACTGTCGATTACTTTCGCTGCAGGTTTGCCTTTAGTTGAGGCAC
TTAAATCGGTGGCAGGGGCGACAGGAAATATAATTTTCGCCAAAGCCACTGATCAAATTAGAGAAGAGGTATCAACCGGT
CAACAAATGAATAAGGCCATGGAAAACACTCAATTATTCCCCAACATGGTAATCCAGATGGTAGCCATTGGAGAAGAGTC
AGGTGCTTTAGAAAAAATGTTAAGTAAGGTTGCTGATTTTTATGAAGAAGATGTGGATAATGCTGTCGATTCACTGAGTA
GTTTATTAGAACCTGTTATTATGGTTGTTTTGGGTGTCTTGGTAGGTGGACTTGTGGTTGCAATGTATTTACCCATCTTC
AAACTGGGATCTGCAATATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0S825

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Legionella pneumophila strain ERS1305867

81.034

100

0.81
  pilC Acinetobacter baumannii D1279779

55.172

100

0.552
  pilC Acinetobacter baylyi ADP1

54.156

97.783

0.53
  pilC Pseudomonas stutzeri DSM 10701

52.463

100

0.525
  pilG Neisseria meningitidis 44/76-A

44.794

100

0.456
  pilG Neisseria gonorrhoeae MS11

44.794

100

0.456
  pilC Vibrio cholerae strain A1552

43.182

97.537

0.421
  pilC Vibrio campbellii strain DS40M4

39.268

100

0.397
  pilC Thermus thermophilus HB27

36.691

100

0.377

Multiple sequence alignment