Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL022_RS10835 Genome accession   NZ_LR134173
Coordinates   2558332..2560053 (-) Length   573 a.a.
NCBI ID   WP_028380562.1    Uniprot ID   A0A0W0S810
Organism   Legionella cherrii strain NCTC11976     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2553332..2565053
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL022_RS10810 (NCTC11976_02251) - 2553370..2553819 (-) 450 WP_028380567.1 CBS domain-containing protein -
  EL022_RS10815 (NCTC11976_02252) - 2554016..2555218 (-) 1203 WP_028380566.1 sterol desaturase family protein -
  EL022_RS10820 (NCTC11976_02253) - 2555234..2556004 (-) 771 WP_028380565.1 HAD-IA family hydrolase -
  EL022_RS10825 (NCTC11976_02254) - 2556115..2556975 (-) 861 WP_028380564.1 A24 family peptidase -
  EL022_RS10830 (NCTC11976_02255) pilC 2557100..2558320 (-) 1221 WP_028380563.1 type II secretion system F family protein Machinery gene
  EL022_RS10835 (NCTC11976_02256) pilB 2558332..2560053 (-) 1722 WP_028380562.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL022_RS10840 (NCTC11976_02257) - 2560360..2561142 (+) 783 WP_028380561.1 methyltransferase domain-containing protein -
  EL022_RS10845 (NCTC11976_02258) - 2561243..2561824 (-) 582 WP_028380560.1 hypothetical protein -
  EL022_RS10850 (NCTC11976_02259) - 2561979..2562305 (+) 327 WP_028380559.1 DUF1820 family protein -
  EL022_RS10855 (NCTC11976_02260) - 2562352..2562912 (+) 561 WP_028380558.1 hypoxanthine-guanine phosphoribosyltransferase -
  EL022_RS10860 (NCTC11976_02261) - 2563234..2564415 (+) 1182 WP_035900468.1 hypothetical protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62878.37 Da        Isoelectric Point: 5.8005

>NTDB_id=1118778 EL022_RS10835 WP_028380562.1 2558332..2560053(-) (pilB) [Legionella cherrii strain NCTC11976]
MLGTDEFKLHGIGQLFVLEKLLDRAQAVEFCKQAAAEKISLVQYLVKNNILSATQIALAASRNFGVPILDLDCIEVDSIP
TSLVNEKLIRRHSMVPLFYRGNNLYLATEDPSIQASLKEIQFHTGLNTHAIVVEADKLSALIDHLLTLQETQGLSEFVGG
DSDEFDGLIINDEEEHETNIATSLTEDAPIVKFVNKILLDAIKQGASDIHFEPYEKEYRIRYRQDGILHEIATPPGSLAT
RITARIKIMSNLDISERRIPQDGGFKMKISKTRSIDFRVSTCPTTSGEKVVMRILDPGSAKLGIEALGFSPIQKEHFVHS
IERPQGMILVTGPTGSGKTVTLYTALNILNTKEVNISTAEDPVEIKVPGINQVNINPKAGLTFSNALRSFLRQDPDIIMV
GEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIATFNVASSVTLIIAQRLARKLCEHCKVLRDDVNKPGL
LELGFTEADIEGIKLYKAGGCPKCNNGYRGRIGLFEVLPMTKTLGQVIMSGGNSLDILKIAQDEGMITVYQSGLEKVKQG
ITTIEEVNRVTVD

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=1118778 EL022_RS10835 WP_028380562.1 2558332..2560053(-) (pilB) [Legionella cherrii strain NCTC11976]
ATGTTAGGAACGGATGAATTCAAATTACATGGAATAGGACAGCTATTTGTTCTTGAAAAGTTGTTAGATAGAGCTCAAGC
TGTCGAATTCTGCAAACAAGCAGCTGCAGAAAAAATCTCTTTGGTTCAATATCTTGTAAAAAATAATATTTTATCAGCCA
CACAAATTGCACTTGCAGCGTCGCGAAATTTTGGCGTTCCCATACTTGATCTTGACTGCATTGAAGTCGATTCTATCCCC
ACTAGTCTGGTTAATGAGAAATTAATTCGTCGACATTCAATGGTTCCCTTATTTTACCGAGGCAACAATTTATACCTAGC
AACAGAAGATCCAAGTATCCAAGCGTCTTTAAAAGAAATTCAATTCCATACTGGGCTCAATACGCATGCAATTGTCGTAG
AGGCTGATAAGCTGAGCGCGCTAATTGATCACTTACTCACATTGCAGGAAACTCAGGGTTTATCGGAGTTTGTTGGTGGC
GATAGTGATGAATTTGATGGACTTATCATTAATGATGAAGAAGAGCATGAAACAAATATTGCAACGTCACTCACCGAAGA
TGCTCCCATTGTAAAATTCGTCAATAAAATACTTCTTGATGCCATTAAACAAGGAGCATCCGACATACATTTTGAACCCT
ACGAGAAAGAATATCGAATACGCTATCGGCAAGATGGTATTTTACACGAAATTGCCACACCGCCAGGAAGTTTGGCTACG
CGAATTACGGCGCGCATAAAAATCATGTCCAACCTAGATATTTCAGAAAGGCGAATACCTCAGGATGGGGGGTTTAAAAT
GAAAATATCCAAAACCCGATCCATCGATTTTCGCGTAAGCACCTGCCCTACTACTTCAGGTGAAAAAGTGGTTATGCGTA
TCTTAGATCCTGGCTCAGCAAAACTTGGTATTGAGGCTTTAGGCTTTAGCCCGATCCAAAAAGAACATTTTGTCCATTCA
ATTGAACGTCCTCAAGGGATGATCCTTGTAACAGGCCCCACCGGTAGTGGTAAAACGGTAACCTTGTATACCGCCTTAAA
TATACTTAATACTAAAGAGGTTAATATCTCCACAGCTGAAGACCCCGTTGAAATTAAAGTGCCAGGGATCAATCAGGTTA
ATATTAATCCCAAAGCAGGTTTAACCTTTTCAAACGCGTTACGATCATTTTTACGTCAAGACCCTGACATCATCATGGTT
GGTGAGATACGTGATTTGGAAACCGCGGAAATAGCAGTTAAAGCAGCGCAAACGGGACACTTGGTACTCTCAACATTGCA
TACAAACAGTGCAGCTGAAACCCTTAATCGTTTGGTTAACATGGGAATCGCGACATTTAATGTAGCCAGTTCAGTAACAC
TTATTATTGCACAACGCTTAGCTCGAAAATTATGCGAACATTGTAAAGTATTGAGAGATGACGTCAATAAACCAGGTTTG
TTGGAATTAGGTTTTACCGAGGCAGATATAGAAGGGATTAAATTATACAAAGCTGGAGGATGTCCTAAATGTAACAATGG
ATACCGCGGTAGAATAGGATTATTTGAAGTATTACCTATGACCAAAACACTTGGACAGGTCATCATGTCCGGAGGGAATT
CATTAGATATATTAAAGATTGCTCAGGATGAAGGCATGATTACTGTTTATCAATCTGGGCTTGAGAAGGTAAAACAAGGT
ATTACAACTATAGAGGAAGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-151)

Type II/IV secretion system protein (T2SSE)

(196-463)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0W0S810

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

79.93

100

0.799
  pilB Acinetobacter baumannii D1279779

51.394

100

0.515
  pilB Acinetobacter baylyi ADP1

51.056

99.127

0.506
  pilB Vibrio campbellii strain DS40M4

48.463

96.51

0.468
  pilB Vibrio parahaemolyticus RIMD 2210633

48.108

96.859

0.466
  pilB Vibrio cholerae strain A1552

47.913

96.161

0.461
  pilF Neisseria gonorrhoeae MS11

46.466

98.778

0.459
  pilF Thermus thermophilus HB27

41.155

93.717

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.076

92.321

0.37

Multiple sequence alignment