Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   EW024_RS00685 Genome accession   NZ_LR130237
Coordinates   105482..106420 (+) Length   312 a.a.
NCBI ID   WP_002987773.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain SP444 isolate SP444     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 100482..111420
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EW024_RS00675 rpoC 101231..104872 (+) 3642 WP_011528160.1 DNA-directed RNA polymerase subunit beta' -
  EW024_RS00680 - 105024..105389 (+) 366 WP_002986560.1 DUF1033 family protein -
  EW024_RS00685 comYA 105482..106420 (+) 939 WP_002987773.1 competence type IV pilus ATPase ComGA Machinery gene
  EW024_RS00690 comYB 106356..107390 (+) 1035 WP_011054115.1 competence type IV pilus assembly protein ComGB Machinery gene
  EW024_RS00695 comYC 107392..107718 (+) 327 WP_011528161.1 competence type IV pilus major pilin ComGC Machinery gene
  EW024_RS00700 comGD 107693..108121 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  EW024_RS00705 comGE 108078..108362 (+) 285 WP_011528162.1 competence type IV pilus minor pilin ComGE -
  EW024_RS00710 comYF 108355..108789 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  EW024_RS00715 comGG 108773..109099 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  EW024_RS00720 comYH 109197..110150 (+) 954 WP_021299429.1 class I SAM-dependent methyltransferase Machinery gene
  EW024_RS00725 - 110209..111405 (+) 1197 WP_011528164.1 acetate kinase -

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35046.30 Da        Isoelectric Point: 8.5799

>NTDB_id=1116540 EW024_RS00685 WP_002987773.1 105482..106420(+) (comYA) [Streptococcus pyogenes strain SP444 isolate SP444]
MVQALAKAILAKAEQVQAQDIYILPRADQYDLFLRIGDERRLVDVYQSDRMAPLISHFKFVAGMIVGEKRRCQVGSCDYK
LSKDKQLSLRLSSVGDYRGQESLVIRLLHHQNKSVHYWFDGLTKVANQVGGRGLYLFAGPVGSGKTTLMYQLISSYHQEA
QVISIEDPVEIKNHQILQLQVNDDIGMTYDNLIKLSLRHRPDILVIGEIRDSQTARAVIRASLTGAMVFSTVHAKSISGV
YARLLELGITKAELSNCLALIAYQRLLNGGALIDSTQNEFEYYSSSNWNQQIDQLLEAGHLNPKQAKLEKII

Nucleotide


Download         Length: 939 bp        

>NTDB_id=1116540 EW024_RS00685 WP_002987773.1 105482..106420(+) (comYA) [Streptococcus pyogenes strain SP444 isolate SP444]
ATGGTACAAGCATTAGCAAAAGCTATTCTAGCAAAAGCTGAACAGGTTCAGGCACAAGATATTTATATTTTGCCAAGAGC
AGATCAATATGATCTTTTTTTACGAATAGGAGATGAAAGGAGATTAGTTGATGTTTATCAGAGCGATCGGATGGCTCCTC
TTATTAGTCACTTTAAATTCGTTGCAGGAATGATAGTTGGTGAAAAGAGACGTTGTCAGGTGGGTTCATGTGATTATAAG
CTTAGTAAAGATAAGCAGTTATCTTTGCGCTTATCTAGCGTGGGTGATTATCGCGGGCAAGAAAGCTTAGTGATTCGTCT
GCTTCATCATCAAAATAAAAGTGTACATTATTGGTTTGATGGATTGACAAAAGTAGCCAATCAAGTTGGCGGTAGAGGGT
TGTATTTATTTGCAGGACCAGTTGGGTCTGGGAAGACAACCTTGATGTACCAGCTGATTTCGAGTTATCATCAAGAAGCA
CAGGTTATTAGTATAGAAGATCCTGTAGAAATTAAAAATCACCAAATTTTACAATTACAAGTGAATGATGATATTGGTAT
GACTTATGACAATTTGATCAAACTGTCTTTACGCCATCGACCAGATATTTTAGTTATTGGTGAGATTCGAGATAGTCAAA
CAGCAAGAGCCGTTATTAGGGCTAGTCTAACAGGTGCCATGGTTTTTTCAACGGTTCACGCTAAAAGTATCTCGGGTGTT
TATGCAAGATTGTTAGAACTTGGTATAACGAAAGCAGAACTGTCTAATTGCTTAGCATTAATTGCTTACCAAAGGTTACT
TAATGGAGGAGCATTGATTGACTCTACTCAAAACGAATTTGAATATTATTCCTCATCGAACTGGAATCAACAAATTGATC
AGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

61.736

99.679

0.615
  comYA Streptococcus mutans UA140

61.736

99.679

0.615
  comYA Streptococcus gordonii str. Challis substr. CH1

60.45

99.679

0.603
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

60.323

99.359

0.599
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

59.486

99.679

0.593
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae D39

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae R6

58.842

99.679

0.587
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

58.842

99.679

0.587
  comGA Lactococcus lactis subsp. cremoris KW2

50.482

99.679

0.503
  comGA Latilactobacillus sakei subsp. sakei 23K

40

92.949

0.372

Multiple sequence alignment