Detailed information NTG1116544

Bioinformatically predicted

Overview

• Name: comYH
• Type: Machinery gene
• Locus tag: EW024_RS00720
• Genome accession: NZ_LR130237
• Coordinates: 109197..110150 (+)
• Length: 317 a.a.
• NCBI ID: WP_021299429.1
• Uniprot ID: A0A660A6K5
• Organism: Streptococcus pyogenes strain SP444 isolate SP444
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 104197..115150

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EW024_RS00680	-	105024..105389 (+)	366	WP_002986560.1	DUF1033 family protein	-
EW024_RS00685	comYA	105482..106420 (+)	939	WP_002987773.1	competence type IV pilus ATPase ComGA	Machinery gene
EW024_RS00690	comYB	106356..107390 (+)	1035	WP_011054115.1	competence type IV pilus assembly protein ComGB	Machinery gene
EW024_RS00695	comYC	107392..107718 (+)	327	WP_011528161.1	competence type IV pilus major pilin ComGC	Machinery gene
EW024_RS00700	comGD	107693..108121 (+)	429	WP_002986548.1	competence type IV pilus minor pilin ComGD	-
EW024_RS00705	comGE	108078..108362 (+)	285	WP_011528162.1	competence type IV pilus minor pilin ComGE	-
EW024_RS00710	comYF	108355..108789 (+)	435	WP_002992738.1	competence type IV pilus minor pilin ComGF	Machinery gene
EW024_RS00715	comGG	108773..109099 (+)	327	WP_010921801.1	competence type IV pilus minor pilin ComGG	-
EW024_RS00720	comYH	109197..110150 (+)	954	WP_021299429.1	class I SAM-dependent methyltransferase	Machinery gene
EW024_RS00725	-	110209..111405 (+)	1197	WP_011528164.1	acetate kinase	-
EW024_RS00730	-	111592..111900 (+)	309	WP_011528165.1	hypothetical protein	-
EW024_RS00735	proC	111983..112753 (-)	771	WP_002986527.1	pyrroline-5-carboxylate reductase	-
EW024_RS00740	pepA	112801..113868 (-)	1068	WP_011528166.1	glutamyl aminopeptidase	-
EW024_RS00750	-	114333..114617 (+)	285	WP_002992749.1	DUF4651 domain-containing protein	-
EW024_RS00755	-	114614..114931 (+)	318	WP_011528167.1	thioredoxin family protein	-

Sequence

Protein

• Length: 317 a.a.
• Molecular weight: 36043.16 Da
• Isoelectric Point: 4.7205

>NTDB_id=1116544 EW024_RS00720 WP_021299429.1 109197..110150(+) (comYH) [Streptococcus pyogenes strain SP444 isolate SP444]
MTFEKIEEAYQLLLENCQLIENDLKTHIYDAIVEQNSFYLGAEGASPQVAQNSDKLKALCLTKEEWRKAYQFLFIKAAQT
EQLQANHQFTPDAIGFILLYLLEQLSDKDSLEVLEIGSGTGNLAQTLLNNTSKSLDYVGIELDDLLIDLSASIAEIMDSS
AHFIQEDAVRPQLLKESDIVISDLPVGYYPNDDIAKRYKVASSDKHTYAHHLLMEQSLKYLKKDGFAIFLVPVNLLTSPQ
SQLLKQWLKDYAQVVTLITLPDSIFGHPSNAKSIIVLQKQTDHPMETFVYPIRDLKLAENIHDFMENFKKWKLSNVN

Nucleotide

• Length: 954 bp

>NTDB_id=1116544 EW024_RS00720 WP_021299429.1 109197..110150(+) (comYH) [Streptococcus pyogenes strain SP444 isolate SP444]
ATGACTTTTGAAAAAATTGAAGAAGCTTATCAGCTGCTCTTAGAGAACTGTCAACTAATTGAAAATGATCTAAAAACACA
TATATACGATGCCATTGTTGAACAAAACTCCTTTTATTTAGGGGCTGAGGGAGCTAGTCCTCAAGTTGCTCAAAATAGTG
ATAAACTGAAAGCCTTGTGCCTGACAAAAGAAGAATGGCGTAAGGCCTACCAGTTTCTTTTTATTAAGGCAGCTCAGACG
GAGCAACTCCAAGCCAACCATCAGTTCACACCAGATGCTATTGGCTTCATTCTGCTGTATCTTTTGGAACAATTGAGTGA
TAAAGATAGCTTAGAGGTACTTGAGATTGGAAGTGGAACAGGGAACCTAGCCCAAACCCTTCTCAACAACACGAGCAAGA
GCCTTGATTATGTAGGGATTGAACTTGATGATCTCTTGATTGATCTGTCAGCCAGTATTGCTGAGATAATGGATTCTTCA
GCTCATTTTATTCAAGAAGATGCGGTAAGGCCTCAATTACTAAAAGAAAGTGACATTGTCATCAGTGACTTACCAGTTGG
TTATTATCCTAACGATGATATTGCTAAACGGTACAAGGTGGCTAGTTCAGATAAGCATACCTATGCCCATCATTTATTAA
TGGAACAGTCTTTAAAATACCTGAAAAAAGACGGTTTTGCGATTTTTCTGGTACCAGTCAATTTATTGACAAGCCCTCAG
AGCCAGTTATTGAAACAGTGGTTAAAAGATTATGCTCAGGTGGTGACCTTGATTACGCTACCAGATTCTATTTTTGGTCA
TCCCTCAAATGCCAAGTCCATTATTGTCTTACAAAAACAAACAGACCACCCAATGGAAACCTTTGTCTATCCAATTCGGG
ATTTGAAGCTTGCAGAGAATATTCATGATTTTATGGAAAATTTCAAAAAATGGAAACTGAGTAATGTCAATTAA

Domains

Predicted by InterproScan.

• N-6 DNA Methylase (N6_Mtase) (68-288)

3D structure

• AlphaFold DB: A0A660A6K5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYH	Streptococcus mutans UA159	65.506	99.685	0.653
comYH	Streptococcus mutans UA140	65.19	99.685	0.65