Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   KKKWG1_RS00325 Genome accession   NZ_LN869922
Coordinates   61339..61809 (-) Length   156 a.a.
NCBI ID   WP_019390097.1    Uniprot ID   B6SE73
Organism   Kingella kingae strain KWG1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 56339..66809
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KKKWG1_RS11060 - 57275..57433 (+) 159 WP_019390092.1 hypothetical protein -
  KKKWG1_RS00295 (KKKWG1_0064) - 57436..58161 (+) 726 WP_019390093.1 metal ABC transporter ATP-binding protein -
  KKKWG1_RS00300 (KKKWG1_0065) - 58210..58602 (+) 393 WP_003791724.1 pyrimidine dimer DNA glycosylase/endonuclease V -
  KKKWG1_RS00305 (KKKWG1_0066) - 58640..59530 (+) 891 WP_019390094.1 metal ABC transporter permease -
  KKKWG1_RS00315 (KKKWG1_0067) - 59976..60752 (-) 777 WP_019390095.1 fimb protein -
  KKKWG1_RS00320 (KKKWG1_0068) - 60764..61192 (-) 429 WP_019390096.1 pilin -
  KKKWG1_RS00325 (KKKWG1_0069) pilE 61339..61809 (-) 471 WP_019390097.1 pilin Machinery gene
  KKKWG1_RS00330 (KKKWG1_0070) recJ 62169..63881 (+) 1713 WP_060536894.1 single-stranded-DNA-specific exonuclease RecJ -
  KKKWG1_RS00335 (KKKWG1_0072) - 63993..64727 (+) 735 WP_003791735.1 histidine phosphatase family protein -
  KKKWG1_RS00340 (KKKWG1_0073) - 64767..65312 (+) 546 WP_019390101.1 gamma carbonic anhydrase family protein -
  KKKWG1_RS00345 (KKKWG1_0074) - 65303..66352 (+) 1050 WP_060536895.1 metallophosphoesterase -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16122.34 Da        Isoelectric Point: 8.9785

>NTDB_id=1114865 KKKWG1_RS00325 WP_019390097.1 61339..61809(-) (pilE) [Kingella kingae strain KWG1]
MKTMQKGFTLIELMIVIAIIGILAAIALPAYQDYTKRSRVSEGLSLAGGVKSALTEYYASNNAWPTDNAAAGVAAKDQIT
GNAVKSVEVGAEGTGAAGTGKITVTFNNKVEDGKTLIMKGVGNDAGSVKWTCTEGNLPTKYRPSECRGTTTATNGG

Nucleotide


Download         Length: 471 bp        

>NTDB_id=1114865 KKKWG1_RS00325 WP_019390097.1 61339..61809(-) (pilE) [Kingella kingae strain KWG1]
ATGAAAACTATGCAAAAAGGTTTCACATTAATCGAGTTGATGATTGTAATCGCTATTATCGGTATTTTGGCTGCGATTGC
ATTGCCAGCTTACCAAGATTACACCAAACGTTCACGCGTATCTGAAGGTTTGAGCTTGGCTGGTGGCGTGAAAAGTGCTT
TGACTGAGTACTACGCTTCTAACAATGCATGGCCTACTGATAATGCGGCAGCAGGTGTTGCAGCTAAAGACCAAATTACA
GGTAATGCAGTAAAAAGCGTAGAAGTTGGTGCAGAGGGTACTGGTGCTGCAGGTACAGGTAAAATTACTGTTACTTTCAA
CAATAAAGTAGAAGATGGTAAGACTTTGATTATGAAAGGCGTAGGCAACGATGCTGGTTCAGTTAAATGGACTTGTACAG
AGGGTAATTTGCCAACTAAATACCGTCCTTCTGAGTGCCGTGGCACTACTACCGCTACCAATGGTGGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(37-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B6SE73

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria gonorrhoeae strain FA1090

48.538

100

0.532
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

41.27

100

0.5
  pilE Neisseria gonorrhoeae MS11

47.561

100

0.5
  pilA2 Legionella pneumophila strain ERS1305867

52.448

91.667

0.481
  pilA2 Legionella pneumophila str. Paris

51.748

91.667

0.474
  pilA Ralstonia pseudosolanacearum GMI1000

39.884

100

0.442
  pilA/pilA1 Eikenella corrodens VA1

41.875

100

0.429
  comP Acinetobacter baylyi ADP1

43.919

94.872

0.417
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.189

94.872

0.372

Multiple sequence alignment