Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   AWM52_RS17850 Genome accession   NZ_LN854573
Coordinates   3934517..3935734 (-) Length   405 a.a.
NCBI ID   WP_059182144.1    Uniprot ID   -
Organism   Pseudomonas sp. URMO17WK12:I11 isolate Shine     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3929517..3940734
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWM52_RS17805 (PSHI_36690) - 3929886..3930059 (-) 174 WP_032833555.1 DUF3094 domain-containing protein -
  AWM52_RS17810 (PSHI_36700) - 3930110..3930637 (-) 528 WP_059182142.1 MOSC domain-containing protein -
  AWM52_RS17815 (PSHI_36710) - 3930634..3931260 (-) 627 WP_027924174.1 DUF1780 domain-containing protein -
  AWM52_RS17820 (PSHI_36720) - 3931389..3931856 (+) 468 WP_027924173.1 hypothetical protein -
  AWM52_RS17825 (PSHI_36730) - 3931853..3932542 (+) 690 WP_027924172.1 energy-coupling factor ABC transporter permease -
  AWM52_RS17830 (PSHI_36740) - 3932606..3932821 (+) 216 WP_027924171.1 hypothetical protein -
  AWM52_RS17835 (PSHI_36750) yacG 3932826..3933026 (-) 201 WP_027924170.1 DNA gyrase inhibitor YacG -
  AWM52_RS17840 (PSHI_36760) coaE 3933023..3933646 (-) 624 WP_027924169.1 dephospho-CoA kinase -
  AWM52_RS17845 (PSHI_36770) pilD 3933643..3934515 (-) 873 WP_059182143.1 A24 family peptidase Machinery gene
  AWM52_RS17850 (PSHI_36780) pilC 3934517..3935734 (-) 1218 WP_059182144.1 type II secretion system F family protein Machinery gene
  AWM52_RS17855 (PSHI_36790) pilB 3935737..3937437 (-) 1701 WP_059182145.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AWM52_RS17860 (PSHI_36800) - 3937663..3938085 (+) 423 WP_027924165.1 pilin -
  AWM52_RS17865 (PSHI_36820) - 3938396..3938749 (+) 354 WP_027924164.1 BON domain-containing protein -
  AWM52_RS17870 (PSHI_36830) - 3938931..3939227 (-) 297 WP_027924163.1 DUF2845 domain-containing protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44350.16 Da        Isoelectric Point: 9.9137

>NTDB_id=1114720 AWM52_RS17850 WP_059182144.1 3934517..3935734(-) (pilC) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
MAVKAAKISVYAWEGTDRKGTKMTGELSGQNPALIKAQLRKQGINPGKVRKKSASFFNLGKRIKAQDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPAMRKLVDEVKQEVAAGNSFAASLRKKPQYFDELYCNLVDAGEQAGALDTLLERVATYKEKS
ESLKAKIKKAMTYPLAVVFVAIIVTGILLVKVVPQFESVFKGFGAELPAFTVMVIGLSEFMQQWWWAMLGGLFAGIFGVR
HAFRKSPGFRDWLDTWLLKLPLIGTLMYKSAVARYARTLSTTFAAGVPLVEALDSVAGATGNIVFKRAVLRIKQDVSTGM
QLNFSMRTSGIFPNMAIQMTAIGEESGALDDMLDKVASFYEDEVDNMVDNLTSLMEPFIMVVLGVIVGGLVVAMYLPIFQ
LGSAI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=1114720 AWM52_RS17850 WP_059182144.1 3934517..3935734(-) (pilC) [Pseudomonas sp. URMO17WK12:I11 isolate Shine]
ATGGCGGTCAAAGCAGCAAAAATCAGCGTCTATGCCTGGGAAGGCACAGACCGCAAAGGCACGAAAATGACCGGCGAGCT
GAGCGGCCAGAACCCGGCGTTGATCAAGGCTCAACTGCGCAAGCAAGGCATCAACCCCGGCAAGGTGCGCAAGAAATCCG
CGTCGTTTTTCAACCTGGGCAAACGCATCAAGGCCCAGGACATAGCCCTGTTCACCCGGCAGATGGCGACCATGATGAAA
GCCGGCGTGCCGCTCCTGCAATCGTTCGACATCATCGGCGAAGGCTTCGATAACCCGGCGATGCGCAAGCTGGTGGACGA
GGTCAAACAGGAAGTGGCGGCGGGTAACAGCTTCGCCGCGTCCCTGCGTAAGAAGCCGCAGTATTTCGATGAGCTTTACT
GCAACCTGGTGGACGCCGGCGAGCAGGCCGGCGCCCTCGATACCCTGTTGGAGCGGGTTGCGACTTACAAGGAAAAGAGC
GAAAGCCTCAAGGCCAAGATCAAGAAAGCCATGACCTACCCGCTGGCGGTGGTCTTCGTCGCGATCATTGTCACCGGGAT
TCTGCTGGTCAAGGTCGTGCCGCAGTTCGAGTCAGTGTTCAAGGGCTTTGGGGCCGAATTGCCGGCGTTCACAGTGATGG
TCATCGGTCTGTCGGAATTCATGCAGCAGTGGTGGTGGGCAATGCTCGGCGGGTTGTTCGCGGGCATTTTCGGTGTGCGT
CACGCCTTCAGGAAATCCCCGGGGTTTCGCGACTGGCTGGACACCTGGCTGTTGAAACTGCCGTTGATTGGCACGCTGAT
GTACAAGTCTGCCGTGGCCCGCTACGCCCGCACGCTGTCGACCACCTTTGCCGCTGGCGTGCCACTGGTCGAAGCGCTGG
ATTCAGTGGCCGGCGCCACCGGCAACATCGTGTTCAAGCGCGCGGTGTTGCGCATCAAGCAGGACGTGTCGACCGGCATG
CAGCTGAATTTTTCCATGCGCACGTCCGGCATCTTTCCCAACATGGCGATTCAGATGACGGCCATTGGCGAAGAGTCCGG
TGCGCTGGACGACATGCTCGACAAGGTCGCGAGCTTCTACGAAGACGAAGTGGACAACATGGTCGATAACCTCACCAGCC
TGATGGAGCCATTCATCATGGTCGTGCTCGGTGTTATCGTCGGAGGCCTTGTGGTTGCGATGTACCTGCCCATCTTCCAA
CTCGGCTCAGCGATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

74.074

100

0.741
  pilC Acinetobacter baylyi ADP1

59.314

100

0.598
  pilC Acinetobacter baumannii D1279779

58.578

100

0.59
  pilC Legionella pneumophila strain ERS1305867

54.293

97.778

0.531
  pilC Vibrio cholerae strain A1552

43.467

98.272

0.427
  pilG Neisseria gonorrhoeae MS11

42.432

99.506

0.422
  pilC Vibrio campbellii strain DS40M4

41.019

100

0.417
  pilG Neisseria meningitidis 44/76-A

41.935

99.506

0.417
  pilC Thermus thermophilus HB27

37.656

99.012

0.373

Multiple sequence alignment