Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AT683_RS03865 Genome accession   NZ_LN831035
Coordinates   759679..761076 (+) Length   465 a.a.
NCBI ID   WP_038440787.1    Uniprot ID   -
Organism   Haemophilus influenzae strain NCTC8143     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 754679..766076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AT683_RS03840 (ERS450003_00771) rsmE 754698..755435 (-) 738 WP_038440782.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AT683_RS03845 (ERS450003_00772) lnt 755488..757014 (-) 1527 WP_080291958.1 apolipoprotein N-acyltransferase -
  AT683_RS03850 (ERS450003_00773) corC 757037..757936 (-) 900 WP_005656283.1 CNNM family magnesium/cobalt transport protein CorC -
  AT683_RS03855 (ERS450003_00774) ampD 758558..759118 (-) 561 WP_011271974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  AT683_RS03860 (ERS450003_00775) pilA 759233..759682 (+) 450 WP_011271973.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AT683_RS03865 (ERS450003_00776) pilB 759679..761076 (+) 1398 WP_038440787.1 GspE/PulE family protein Machinery gene
  AT683_RS03870 (ERS450003_00777) pilC 761069..762289 (+) 1221 WP_011271971.1 type II secretion system F family protein Machinery gene
  AT683_RS03875 (ERS450003_00778) pilD 762286..762978 (+) 693 WP_038440789.1 A24 family peptidase Machinery gene
  AT683_RS03880 (ERS450003_00779) rho 763032..764294 (-) 1263 WP_005629550.1 transcription termination factor Rho -
  AT683_RS03885 (ERS450003_00780) metJ 764542..764859 (+) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  AT683_RS03890 (ERS450003_00781) cueR 764873..765259 (-) 387 WP_005663006.1 Cu(I)-responsive transcriptional regulator -
  AT683_RS03895 (ERS450003_00782) - 765336..765542 (+) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  AT683_RS03900 (ERS450003_00783) - 765617..765823 (+) 207 WP_005686717.1 heavy-metal-associated domain-containing protein -

Sequence

Protein


Download         Length: 465 a.a.        Molecular weight: 53114.46 Da        Isoelectric Point: 5.6093

>NTDB_id=1114281 AT683_RS03865 WP_038440787.1 759679..761076(+) (pilB) [Haemophilus influenzae strain NCTC8143]
MTSYALLHTQRVTAQNGEIFTISPDLWERNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETIAFITGKPVEPIL
LESSQLKELLQQLTPHQMQVEEQVKFYQHQETHFEQEDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRIDGVL
QPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFAELG
MTENQQQAFQRSLSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSRLLR
AFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQHEIENSLLLVIAQRLVRKICPKC
GGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKTHD

Nucleotide


Download         Length: 1398 bp        

>NTDB_id=1114281 AT683_RS03865 WP_038440787.1 759679..761076(+) (pilB) [Haemophilus influenzae strain NCTC8143]
ATGACGAGCTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGCCAGATTTATG
GGAACGCAATCAGCAGCAACAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTGCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGTGGAAGAGCAAGTTAAATTCTA
TCAACATCAAGAAACCCATTTTGAACAAGAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTGAATCTGCCT
TACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGATGGTGTTTTA
CAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATTAGATATTAG
TGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCCTTTCAACCT
TACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCTGAACTGGGT
ATGACCGAAAATCAGCAACAAGCATTTCAACGCTCACTTAGCCAGCCACAAGGATTAATTTTAGTAACCGGCCCCACAGG
AAGTGGGAAAAGTATCTCGCTTTACACCGCACTTCAGTGGCTAAATACGCCTGATAAACATATTATGACCGCTGAAGATC
CCATTGAAATTGAACTTGATGGTATTATTCAAAGCCAAATTAATCCGCAGATTGGATTAGATTTTAGCCGTCTATTGCGT
GCTTTTTTACGTCAAGATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAAAGTGCAAGGATTGCACTACGTGC
CGCTCAAACGGGACATTTGGTGCTTTCAACTTTACATACCAATGATGCAATATCTGCCATTTCTCGCTTACAACAACTCG
GTATTCAACAACATGAAATTGAAAACAGTTTACTACTCGTCATTGCACAGCGTCTTGTACGAAAAATCTGTCCAAAGTGC
GGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCTACATTGGCA
ACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTGGAAAAAGTTAGCCAAGGCATAACAGATGAGA
AAGAAATTGAACGTGTGTTAGGTAAAACTCATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-98)

Type II/IV secretion system protein (T2SSE)

(128-395)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

99.784

99.57

0.994
  pilB Haemophilus influenzae Rd KW20

96.537

99.355

0.959
  pilB Glaesserella parasuis strain SC1401

57.456

98.065

0.563
  pilB Vibrio cholerae strain A1552

39.658

100

0.449
  pilB Vibrio campbellii strain DS40M4

40.082

100

0.422
  pilB Vibrio parahaemolyticus RIMD 2210633

39.959

100

0.415
  pilB Legionella pneumophila strain ERS1305867

39.468

100

0.415
  pilB Acinetobacter baylyi ADP1

38.105

100

0.406
  pilB Acinetobacter baumannii D1279779

38.669

100

0.4
  pilF Neisseria gonorrhoeae MS11

36.495

100

0.381
  pilF Thermus thermophilus HB27

36.232

100

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.393

100

0.376

Multiple sequence alignment