Detailed information NTG1112614

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KHO07_RS12895
• Genome accession: NZ_HG995459
• Coordinates: 2833150..2834259 (+)
• Length: 369 a.a.
• NCBI ID: WP_212627413.1
• Uniprot ID: -
• Organism: Pseudomonas sp. PP3
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2828150..2839259

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KHO07_RS12880	-	2829026..2829958 (+)	933	WP_212627411.1	sugar kinase	-
KHO07_RS12885	-	2830171..2830446 (-)	276	WP_007933743.1	peptidylprolyl isomerase	-
KHO07_RS12890	-	2830501..2833038 (-)	2538	WP_212627412.1	PAS domain-containing protein	-
KHO07_RS12895	pilU	2833150..2834259 (+)	1110	WP_212627413.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KHO07_RS12900	-	2834285..2836240 (-)	1956	WP_212627414.1	acetoacetate--CoA ligase	-
KHO07_RS12905	-	2836417..2837190 (-)	774	WP_212627415.1	3-hydroxybutyrate dehydrogenase	-
KHO07_RS12910	-	2837198..2838589 (-)	1392	WP_212627416.1	GntP family permease	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40881.81 Da
• Isoelectric Point: 6.5076

>NTDB_id=1112614 KHO07_RS12895 WP_212627413.1 2833150..2834259(+) (pilU) [Pseudomonas sp. PP3]
MEIDALLKRLASQDGSDLYLSTGAPPSARFDGVLTPLADPPFKPGEIAAIAASIMDAEQRLEFDRQLEMNLAISLPGIGR
FRVNIFKQRNDTSMVVRNVKLDIPRYEDLKLPPVLLETVMLKQGLMLFVGATDSGKSTSLAALIDYRNRHASGHIVTIED
PIEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRDTMEHALAFADSGHLVISTLHAHNANQALDRM
INFFPEERRGQLLNDLGNNLKACVSQRLVRSRDGQRRVAVEVMVGTPTIGDLIRRNAFSELKGVMEKSTALGMQTFDGAL
FELVVEGVIDEEEALKHADSVNNLRLRLKLHAEATPNAHAPPGDWGLVD

Nucleotide

• Length: 1110 bp

>NTDB_id=1112614 KHO07_RS12895 WP_212627413.1 2833150..2834259(+) (pilU) [Pseudomonas sp. PP3]
ATGGAAATCGATGCATTGTTGAAACGCCTGGCCAGCCAGGATGGCTCCGATCTCTACCTGTCCACCGGCGCACCACCCAG
TGCGCGGTTCGACGGTGTTCTGACACCCCTTGCCGACCCGCCCTTCAAGCCGGGAGAAATTGCAGCGATTGCCGCGTCCA
TCATGGACGCCGAACAGCGACTGGAGTTCGATCGTCAGCTGGAGATGAACCTGGCGATCTCCCTGCCGGGAATTGGTCGC
TTCCGGGTCAATATTTTCAAGCAGCGCAATGACACTTCAATGGTGGTGCGCAACGTCAAACTCGACATCCCGCGCTACGA
AGACCTCAAGCTGCCGCCGGTATTGCTGGAAACGGTGATGCTCAAGCAAGGCTTGATGTTGTTCGTCGGCGCCACCGATT
CCGGCAAGTCTACCTCGCTGGCGGCCCTGATCGATTATCGCAACCGGCATGCCAGTGGCCATATCGTCACCATTGAGGAC
CCCATCGAGTATATCCACCGGCACAAGAAATCCATCATTAACCAGCGCGAGGTTGGCGTCGACACCCGCAGTTTTCATGC
CGCGTTGAAAAACACCCTGCGTCAGGCGCCGGACGTGGTACTGATCGGCGAGATCCGTGATCGCGACACCATGGAGCACG
CGCTGGCGTTTGCCGACTCCGGGCATCTGGTGATCTCCACGCTGCACGCGCACAACGCCAATCAGGCACTGGACCGCATG
ATCAACTTCTTTCCCGAGGAGCGGCGCGGGCAATTGCTCAATGATCTGGGCAACAATCTCAAGGCCTGTGTTTCCCAACG
GCTGGTGCGCAGCCGCGATGGACAACGACGGGTGGCAGTGGAGGTGATGGTGGGAACGCCGACGATCGGCGATCTGATTC
GGCGCAACGCGTTTTCCGAATTGAAGGGCGTGATGGAGAAATCAACGGCGCTGGGGATGCAGACGTTCGATGGGGCCCTG
TTCGAACTGGTCGTCGAAGGCGTGATTGATGAGGAAGAGGCGCTCAAACATGCCGATTCGGTGAACAACTTGCGCTTGCG
TTTGAAACTACATGCCGAAGCCACACCCAACGCCCACGCACCGCCAGGCGATTGGGGACTCGTCGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (78-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.472	100	0.545
pilU	Acinetobacter baylyi ADP1	51.685	96.477	0.499
pilU	Vibrio cholerae strain A1552	49.143	94.851	0.466
pilT	Pseudomonas aeruginosa PAK	39.826	93.225	0.371
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Legionella pneumophila strain Lp02	40.12	90.515	0.363
pilT	Legionella pneumophila strain ERS1305867	40.12	90.515	0.363
pilT	Vibrio cholerae strain A1552	40.24	90.244	0.363
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.24	90.244	0.363