Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KHN79_RS11045 Genome accession   NZ_HG992749
Coordinates   2452530..2454215 (+) Length   561 a.a.
NCBI ID   WP_182008845.1    Uniprot ID   -
Organism   Vibrio sp. B1FLJ16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2447530..2459215
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KHN79_RS11025 (ACOMICROBIO_LOCUS1622) pdhR 2448805..2449572 (-) 768 WP_182008842.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  KHN79_RS11030 ampD 2449983..2450527 (-) 545 Protein_2147 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KHN79_RS11035 (ACOMICROBIO_LOCUS1625) nadC 2450913..2451800 (+) 888 WP_182008843.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KHN79_RS11040 (ACOMICROBIO_LOCUS1626) pilA 2452072..2452530 (+) 459 WP_182008844.1 pilin Machinery gene
  KHN79_RS11045 (ACOMICROBIO_LOCUS1627) pilB 2452530..2454215 (+) 1686 WP_182008845.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KHN79_RS11050 (ACOMICROBIO_LOCUS1628) pilC 2454246..2455469 (+) 1224 WP_182008846.1 type II secretion system F family protein Machinery gene
  KHN79_RS11055 (ACOMICROBIO_LOCUS1629) pilD 2455540..2456409 (+) 870 WP_182008847.1 A24 family peptidase Machinery gene
  KHN79_RS11060 (ACOMICROBIO_LOCUS1630) coaE 2456410..2457024 (+) 615 WP_182008848.1 dephospho-CoA kinase -
  KHN79_RS11065 (ACOMICROBIO_LOCUS1631) zapD 2457052..2457792 (+) 741 WP_182008849.1 cell division protein ZapD -
  KHN79_RS11070 (ACOMICROBIO_FLGHMIGD_02242) yacG 2457897..2458091 (+) 195 WP_014232956.1 DNA gyrase inhibitor YacG -
  KHN79_RS11075 (ACOMICROBIO_LOCUS1632) rplS 2458462..2458815 (-) 354 WP_145532528.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 61774.54 Da        Isoelectric Point: 5.4966

>NTDB_id=1112437 KHN79_RS11045 WP_182008845.1 2452530..2454215(+) (pilB) [Vibrio sp. B1FLJ16]
MLTNLSTILRQSGLLSLAQEESLLEQVNASGISMPEALVRSGYLTTDELLAQLSETFGLPSCKLSDYDFTALCQQLGLRE
LITRHHAIPVQKTSSTLILAVADPTNQQAENDFRFASGLQVDVVIADFADIDAAIRRLYGRSIGQEKPGLKEINQDELAN
LVDIGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSAASLDIDKLGYSDQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLNILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKAAQTGHLVLSTLHTNSAAETVVRLSNMGVESFNLASSLSLIIAQRLARKLCSHCKQPQELTAALQHVGIHSTEN
IFQANPHGCNACTHGYSGRTGIYEVMHFNESLSEALIKGASVHELEKLAISNGMSTLQMSGLEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1112437 KHN79_RS11045 WP_182008845.1 2452530..2454215(+) (pilB) [Vibrio sp. B1FLJ16]
ATGCTCACCAACCTCTCTACGATTCTTCGCCAATCTGGGTTACTTAGCTTAGCCCAAGAAGAATCGTTACTAGAGCAAGT
CAACGCTTCTGGCATTTCGATGCCGGAGGCGTTAGTTCGTTCTGGCTATCTCACAACCGATGAACTGCTCGCCCAGCTTA
GTGAAACCTTTGGCCTGCCCTCGTGCAAGCTGTCTGACTACGACTTTACCGCCCTATGTCAGCAGCTCGGCCTGCGTGAA
CTGATCACTCGCCATCATGCCATTCCTGTCCAGAAGACTTCATCGACGTTAATCCTAGCAGTTGCCGACCCAACCAATCA
GCAAGCTGAAAACGATTTTCGCTTTGCCTCCGGATTACAGGTCGATGTGGTTATTGCTGACTTTGCCGACATTGATGCCG
CTATTCGGCGATTGTATGGCCGCTCGATTGGTCAGGAAAAACCGGGCTTAAAAGAGATCAACCAGGACGAGCTGGCAAAC
CTTGTTGATATCGGTGCCGACGAAATCGACAACATCGAAGATTTAAGCCAGGACGAATCTCCGGTCAGTCGCTATATCAA
CCAGATTTTATTGGATGCGGTGCGTAAAGGCGCATCAGATATCCACTTTGAACCGTATGAAAAAATGTACCGCGTGCGTC
TGCGTTGTGACGGTATTTTGATTGAAACCCAGCAACCTCCCAGTCACTTAAGCCGCCGCTTATCAGCGCGGATCAAAATA
CTGTCTAAACTCGATATTGCCGAAAGGCGTTTGCCCCAAGACGGACGAATCAAGTTAAAACTCAATCAAGACACCGCGAT
TGATATGCGGGTATCGACCCTGCCTACTCTGTTTGGTGAAAAGATTGTACTGCGTCTGCTCGACAGCAGTGCTGCATCAC
TGGATATCGATAAGCTCGGTTACAGTGATCAACAAAAACAGCTGTATTTAGATGCTCTGCGTCGCCCTCAGGGAATGATT
TTGATGACTGGCCCGACCGGGAGTGGTAAAACCGTCTCGCTATACACAGGGTTAAATATTCTCAATAAACCAGAAATCAA
TATCTCTACTGCGGAAGATCCGGTAGAAATCAACTTATCCGGCATTAATCAGGTTCAGGTTCAGCCTAAAATTGGCTTTG
GTTTTGCTGAGGCGTTGCGATCCTTCCTGCGTCAAGACCCGGATGTGGTGATGGTCGGGGAAATCCGTGACCTCGATACC
GCAGAAATCGCAATTAAAGCCGCACAAACCGGTCACTTAGTGCTTTCTACCCTGCATACCAACTCTGCTGCCGAAACCGT
GGTTCGTCTGTCCAACATGGGGGTCGAAAGCTTTAACCTCGCCTCTTCCCTAAGCCTTATCATTGCCCAACGCTTGGCAC
GAAAACTTTGCTCTCACTGCAAGCAGCCACAAGAACTTACCGCTGCTTTACAACATGTCGGTATTCACTCCACCGAAAAC
ATTTTTCAAGCTAACCCACACGGATGCAATGCATGCACTCACGGCTACTCTGGCCGTACCGGTATTTATGAAGTGATGCA
CTTTAATGAATCCTTATCAGAAGCACTTATCAAAGGCGCTTCAGTACACGAACTGGAAAAGCTCGCCATCAGCAATGGCA
TGAGCACCCTACAAATGTCCGGTTTAGAGAAACTCAAACAAGGAATCACCAGCTTTAGTGAGTTACAACGCGTGCTCTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

86.631

100

0.866
  pilB Vibrio campbellii strain DS40M4

85.561

100

0.856
  pilB Vibrio cholerae strain A1552

73.843

100

0.74
  pilB Acinetobacter baumannii D1279779

50.471

94.652

0.478
  pilB Legionella pneumophila strain ERS1305867

49.351

96.078

0.474
  pilB Acinetobacter baylyi ADP1

50.586

91.266

0.462
  pilF Neisseria gonorrhoeae MS11

44.504

100

0.447
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.544

100

0.376
  pilF Thermus thermophilus HB27

36.702

100

0.369
  pilB/pilB1 Synechocystis sp. PCC 6803

37.297

98.93

0.369

Multiple sequence alignment