Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KJB05_RS03530 Genome accession   NZ_HG992742
Coordinates   739331..741016 (-) Length   561 a.a.
NCBI ID   WP_208445168.1    Uniprot ID   -
Organism   Vibrio sp. B1ASS3     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 734331..746016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KJB05_RS03505 (ACOMICROBIO_NCLOACGD_00716) yacG 735492..735692 (-) 201 WP_027726863.1 DNA gyrase inhibitor YacG -
  KJB05_RS03510 (ACOMICROBIO_LOCUS458) zapD 735749..736489 (-) 741 WP_038897986.1 cell division protein ZapD -
  KJB05_RS03515 (ACOMICROBIO_LOCUS459) coaE 736517..737131 (-) 615 WP_208445166.1 dephospho-CoA kinase -
  KJB05_RS03520 (ACOMICROBIO_LOCUS460) pilD 737132..738001 (-) 870 WP_208445167.1 A24 family peptidase Machinery gene
  KJB05_RS03525 (ACOMICROBIO_LOCUS461) pilC 738074..739297 (-) 1224 WP_009707569.1 type II secretion system F family protein Machinery gene
  KJB05_RS03530 (ACOMICROBIO_LOCUS462) pilB 739331..741016 (-) 1686 WP_208445168.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KJB05_RS03535 (ACOMICROBIO_LOCUS463) pilA 741016..741420 (-) 405 WP_038878904.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KJB05_RS03540 (ACOMICROBIO_LOCUS464) nadC 741685..742572 (-) 888 WP_208445169.1 carboxylating nicotinate-nucleotide diphosphorylase -
  KJB05_RS03545 (ACOMICROBIO_LOCUS465) ampD 742665..743216 (+) 552 WP_208445170.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  KJB05_RS03550 (ACOMICROBIO_LOCUS466) pdhR 743621..744388 (+) 768 WP_005438026.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62240.18 Da        Isoelectric Point: 5.6668

>NTDB_id=1112389 KJB05_RS03530 WP_208445168.1 739331..741016(-) (pilB) [Vibrio sp. B1ASS3]
MLTNLSTVLRQAGILTFSQEELLLEQAKASGISMPEALLNSGFFQSHELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNLQAEDDFRFATGLQVELVLADFRELTAAIRRLYGRALGQEKSRLKEINQDELAS
LVEVGDDEIENIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYENMYRVRLRCDGILIEIQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAVDMRVSTLPTLFGEKIVLRLLDSSSAALDINKLGYSDQQKQLYLDALRRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLPGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCPHCRQPQEPNAQLQHIGITNTEH
VFKANPDGCNECTHGYSGRTGIYEVMKFDESLSEALIKGVSVHELEKLAIAKGMQTLQMSGIEKLKQGVTSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=1112389 KJB05_RS03530 WP_208445168.1 739331..741016(-) (pilB) [Vibrio sp. B1ASS3]
GTGCTAACCAACCTCTCAACAGTTCTTCGCCAAGCGGGGATACTCACCTTTTCCCAAGAAGAGCTGTTACTTGAGCAAGC
CAAAGCTTCTGGTATATCAATGCCAGAAGCTTTACTCAATTCTGGCTTTTTCCAATCTCATGAATTAACGGAGCACCTAA
GCTCTGTTTTCGGGCTTTCTTGCACTTCTCTCAATCAATACGAATACGCTTCGTTATGCCAAACGCTAGGTTTGCGAGAT
TTGATCACTCGGCACAACGCTCTGCCGCTTAATCGCACGTCATCAACTTTGATTCTCGCGGTTGCCGATCCAACCAACCT
TCAAGCTGAAGACGACTTTCGTTTTGCGACAGGCTTGCAAGTCGAACTTGTTCTGGCTGATTTTCGCGAGCTGACCGCTG
CGATTCGGCGCTTATATGGCCGAGCACTTGGGCAGGAAAAATCGAGGTTAAAAGAAATAAACCAAGACGAGCTCGCAAGC
TTGGTTGAGGTTGGAGATGATGAGATTGAGAACATTGAAGATCTCAGTCAAGACGAATCCCCCGTCAGTCGTTATATCAA
CCAAATCTTACTCGATGCTGTACGCAAAGGGGCTTCCGATATTCACTTCGAACCCTACGAAAACATGTATCGAGTCCGTT
TACGCTGCGACGGCATCTTAATTGAAATTCAACAGCCTCCTAGCCATTTAAGCCGTCGTCTCTCTGCCCGAATAAAGATC
CTATCTAAGCTCGATATTGCTGAGCGCCGTTTGCCGCAGGATGGGCGCATCAAACTCAAATTAAACCAAGACACCGCGGT
TGATATGCGTGTCTCCACACTGCCAACTCTGTTTGGAGAAAAGATCGTACTGCGTTTGCTAGACAGCAGTTCTGCAGCAT
TAGATATCAATAAACTTGGTTATAGCGATCAACAGAAACAGCTTTATTTGGATGCTCTGCGCCGGCCACAAGGCATGATT
CTGATGACAGGTCCAACAGGTAGTGGCAAAACCGTTTCTCTTTATACAGGGCTAAGCATTCTTAACAAACCAGAGATCAA
CATCTCGACTGCGGAAGACCCTGTGGAGATAAACTTACCCGGCATTAACCAAGTTCAAGTTCAGCCGAAGATCGGTTTTG
GTTTTGCTGAAGCACTTCGTTCTTTCTTGCGTCAGGATCCTGATGTTGTCATGGTGGGTGAGATCCGGGACCTCGATACG
GCAGAAATCGCGATCAAAGCATCGCAAACTGGTCACTTGGTTCTTTCCACTTTACATACCAACTCGGCCGCTGAGACCGT
AATCCGCTTATCCAACATGGGTGTAGAAAGCTTTAACCTTGCCTCCTCACTCAGTTTGATCATCGCCCAACGGTTAGCAC
GCAAATTGTGCCCGCACTGTAGACAACCGCAAGAGCCAAATGCCCAACTGCAACATATTGGGATTACCAACACAGAGCAT
GTCTTTAAAGCCAATCCAGATGGTTGTAACGAGTGTACCCACGGTTATTCAGGTCGAACGGGCATCTATGAAGTGATGAA
GTTTGATGAGTCCCTATCTGAGGCGCTCATAAAAGGAGTTTCAGTACACGAATTAGAAAAACTCGCCATCGCCAAAGGGA
TGCAAACCCTACAAATGTCTGGCATCGAAAAACTCAAACAAGGCGTCACTAGCTTCAGCGAATTGCAACGCGTTCTCTAC
TTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-142)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

96.078

100

0.961
  pilB Vibrio parahaemolyticus RIMD 2210633

89.127

100

0.891
  pilB Vibrio cholerae strain A1552

73.488

100

0.736
  pilB Acinetobacter baumannii D1279779

51.252

92.513

0.474
  pilB Acinetobacter baylyi ADP1

48.339

96.613

0.467
  pilB Legionella pneumophila strain ERS1305867

48.339

96.613

0.467
  pilF Neisseria gonorrhoeae MS11

43.262

100

0.435
  pilB/pilB1 Synechocystis sp. PCC 6803

36.869

100

0.39
  pilF Thermus thermophilus HB27

36.879

100

0.371
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.38

99.465

0.362

Multiple sequence alignment